High performance nitrogen removal through integrating denitrifying anaerobic methane oxidation and Anammox: from enrichment to application

Nie, Wen-Bo; Xie, Guo-Jun; Ding, Jie; Lü, Yang; Liu, Bingfeng; Xing, Defeng; Wang, Qilin; Han, Hongjun; Yuan, Zhiguo; Ren, Nanqi

doi:10.1016/j.envint.2019.105107

Cited by 57 publications

(37 citation statements)

References 49 publications

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“…As shown in Table 4, in an MBfR reactor, with biofilms consisting of a coculture of DAMO and anammox microorganisms, a NRR of 0.68 kg/m 3 /d was achieved, indicating that the nitrate reduction rate achieved by the DAMO process can be high enough to remove the nitrate produced by the anammox process, therefore enabling complete nitrogen removal from wastewater (Cai et al, 2015). In a two-stage PN-DAMO/A process, a high performance of NRR, at 2.5 kg/m 3 /d, was reported within 200 days operation, indicating the potential of the DAMO/ A process in wastewater treatment applications (Nie et al, 2019). In another two-stage PN-DAMO/A process, with an HRT of 1 day, the process achieved an NRR of over 1 kg /m 3 /d, and nitrite and ammonium removal rates of 0.56 kg/m 3 /d and 0.47 kg/m 3 /d, respectively, with no nitrate accumulation (Xie et al, 2017).…”

Section: Damo/a Processmentioning

confidence: 85%

“…4a. It can also be applied to the two-stage PN and DAMO/A (PN-DAMO/A) process (Nie et al, 2019;Liu et al, 2021Liu et al, , 2020, as shown in Fig. 4b.…”

Section: Damo/a Processmentioning

confidence: 99%

“…4b. This design is based on the DAMO microbes and the anammox bacteria, both of which require an anaerobic environment (Nie et al, 2019). As shown in Table 4, in an MBfR reactor, with biofilms consisting of a coculture of DAMO and anammox microorganisms, a NRR of 0.68 kg/m 3 /d was achieved, indicating that the nitrate reduction rate achieved by the DAMO process can be high enough to remove the nitrate produced by the anammox process, therefore enabling complete nitrogen removal from wastewater (Cai et al, 2015).…”

Section: Damo/a Processmentioning

confidence: 99%

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The main anammox-based processes, the involved microbes and the novel process concept from the application perspective

Guo

Luo

Shen

et al. 2021

Front. Environ. Sci. Eng.

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Anammox technology has been widely researched over the past 40-year from the laboratory-scale to full-scale. It is well-known that in actual applications, the solo application of anammox is not feasible. Since both ammonium and nitrite are prerequisites based on the reaction mechanism, the pre-treatment of wastewater is necessary. With the combination of anammox process and other pre-treatment processes to treat the actual wastewater, many types of anammox-based processes have been developed with distinct nitrogen removal performance. Thus, in order to heighten the awareness of researchers to the developments and accelerate the application of these processes to the treatment of actual wastewater, the main anammox-based processes are reviewed in this paper. It includes the partial nitritation/anammox process, the denitratation/anammox (PD/A) process, the denitrifying anaerobic methane oxidation/anammox (DAMO/A) process, and more complex deuterogenic processes. These processes have made the breakthroughs in the application of the anammox technology, such as the combination of nitrification and PD/A process can achieve stability and reliability of nitrogen removal in the treatment of mainstream wastewater, the PD/A process and the DAMO/A have brought about further improvements in the total nitrogen removal efficiency of wastewater. The diversity of functional microbe characteristics under the specific condition indicate the wide application potential of anammox-based processes, and further exploration is necessary. A whole waste treatment system concept is proposed through the effective allocation of above mentioned processes, with the maximum recovery of energy and resources, and minimal environmental impact.

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Section: Damo/a Processmentioning

confidence: 85%

“…4a. It can also be applied to the two-stage PN and DAMO/A (PN-DAMO/A) process (Nie et al, 2019;Liu et al, 2021Liu et al, , 2020, as shown in Fig. 4b.…”

Section: Damo/a Processmentioning

confidence: 99%

Section: Damo/a Processmentioning

confidence: 99%

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The main anammox-based processes, the involved microbes and the novel process concept from the application perspective

Guo

Luo

Shen

et al. 2021

Front. Environ. Sci. Eng.

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“…In the context of global warming and the increasingly signi cant impact of human activities on the environment, greenhouse gas emission reduction, low-carbon economy and low-carbon cities are the research hotspots nowadays [20] , [34] . Global climate change caused by the increase of greenhouse gas emissions in the atmosphere has become the focus of human concern, and is also a huge challenge facing the sustainable development of the world economy.…”

Section: Introductionmentioning

confidence: 99%

Application of Bioelectrochemical Technique in Energy Recovery and CH4 Control From Constructed Wetland (CW) and Associated Biochemical Mechanisms

Zhang

Wang

et al. 2021

Preprint

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In the context of global warming, the bioelectrochemical method (microbial fuel cell MFC) was proposed for CH4 control from CWs. The main focus is to further explore the effect of plant roots location at the electrode, plant species on CH4 emissions, bioelectricity generation and the mechanism underlying competition between electrogenesis and methanogenesis at the anode. The results showed that the operation of MFC effectively reduced the CH4 emissions and promoted COD removal rates. CH4 emission was significantly higher in open circuit (6.2 mg m-2 h-1) than in closed circuit reactors (3.1 mg m-2 h-1). Plant roots at the cathode had the highest electricity generation and the lowest CH4 emissions. The highest power generation (0.49 V, 0.33 w m-3) and the lowest CH4 emissions (2.3 mg m-2 h-1) were observed in the reactors where Typha Orientalis was planted with plant roots at the cathode. The role of plants in strengthening electron acceptor was greater than that of plant rhizodeposits in strengthening electron donors. q-PCR and correlation analysis indicated that the mcrA genes and CH4 emissions were positively correlated (r=0.98, p<0.01), while no significant relationship between CH4 emissions and pmoA genes was observed. More nanowires, which are conductive to electron transfer, were found when plant roots were in cathode by scanning electron microscope (SEM). Illumina sequencing revealed that more abundant exoelectrogens and denitrifying bacteria (Geobacter, Desulfobulbu, Nitrospira and Anaerolinea) were observed when plant roots located in cathodes. Strictly acetotrophic archae (Methanosaetaceae) were likely main electron donor competitors with exoelectrogens. In addition, plant species played a more important role in CH4 emissions and electricity generation than the plant roots location at the electrode. Therefore, it is necessary to strengthen plant configuration to reduce CH4 emissions, so as to promote sustainable development of wastewater treatment.

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“…Methanoperedens’ archaea are very useful partners for both ‘ Ca . Methylomirabilis’ and anammox bacteria (Haroon et al 2013 ; Shen et al 2014 ; Meng et al 2016 ; Arshad et al 2017 ; Xu et al 2017 ; Fu et al 2019 ; Nie et al 2019 , 2020 ). This is supported by studies showing these anaerobic methane- and ammonium-oxidizing microorganisms living together in oxygen-limited ecosystems (reviewed in Welte et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

Stultiens

Kessel

Frank

et al. 2020

Appl Microbiol Biotechnol

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Anaerobic wastewater treatment offers several advantages; however, the effluent of anaerobic digesters still contains high levels of ammonium and dissolved methane that need to be removed before these effluents can be discharged to surface waters. The simultaneous anaerobic removal of methane and ammonium by denitrifying (N-damo) methanotrophs in combination with anaerobic ammonium-oxidizing (anammox) bacteria could be a potential solution to this challenge. After a molecular survey of a wastewater plant treating brewery effluent, indicating the presence of both N-damo and anammox bacteria, we started an anaerobic bioreactor with a continuous supply of methane, ammonium, and nitrite to enrich these anaerobic microorganisms. After 14 months of operation, a stable enrichment culture containing two types of 'Candidatus Methylomirabilis oxyfera' bacteria and two strains of 'Ca. Brocadia'-like anammox bacteria was achieved. In this community, anammox bacteria converted 80% of the nitrite with ammonium, while 'Ca. Methylomirabilis' contributed to 20% of the nitrite consumption. The analysis of metagenomic 16S rRNA reads and fluorescence in situ hybridization (FISH) correlated well and showed that, after 14 months, 'Ca. Methylomirabilis' and anammox bacteria constituted approximately 30 and 20% of the total microbial community. In addition, a substantial part (10%) of the community consisted of Phycisphaera-related planctomycetes. Assembly and binning of the metagenomic sequences resulted in high-quality draft genome of two 'Ca. Methylomirabilis' species containing the marker genes pmoCAB, xoxF, and nirS and putative NO dismutase genes. The anammox draft genomes most closely related to 'Ca. Brocadia fulgida' included the marker genes hzsABC, hao, and hdh. Whole-reactor and batch anaerobic activity measurements with methane, ammonium, nitrite, and nitrate revealed an average anaerobic methane oxidation rate of 0.12 mmol h −1 L −1 and ammonium oxidation rate of 0.5 mmol h −1 L −1. Together, this study describes the enrichment and draft genomes of anaerobic methanotrophs from a brewery wastewater treatment plant, where these organisms together with anammox bacteria can contribute significantly to the removal of methane and ammonium in a more sustainable way. Key points • An enrichment culture containing both N-damo and anammox bacteria was obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth metagenomic biodiversity analysis of inoculum and enrichment culture.

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High performance nitrogen removal through integrating denitrifying anaerobic methane oxidation and Anammox: from enrichment to application

Cited by 57 publications

References 49 publications

The main anammox-based processes, the involved microbes and the novel process concept from the application perspective

The main anammox-based processes, the involved microbes and the novel process concept from the application perspective

Application of Bioelectrochemical Technique in Energy Recovery and CH4 Control From Constructed Wetland (CW) and Associated Biochemical Mechanisms

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

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