High performance reversed‐phase chromatography of natural triglyceride mixtures

Abstract

High performance reversed-phase chromatography (HPRC) is an efficient and powerful tool gaining momentum in the separation of triglycerides and other lipid components. In the present study the effect of different variables in triglyceride separation has been studied. It was found that a longer hydrocarbon chain bonded to silica gel as well as the percent coverage improved the separation. Smaller particle size packing increased triglyceride resolution. A decrease in mobile phase polarity by increasing the acetone content resulted in a decrease in the retention time and resolution of triglyceride.

Effects of Triacylglycerol Molecular Species on the Oxidation Behavior of Oils Containing α-Linolenic Acid

“…The mobile phase was a mixture of acetone-acetonitrile 1:1, v/v , and the flow rate was 0.8 mL/min. TAG molecular species were identified using equivalent carbon number ECN and theoretical carbon number TCN of TAGs as described in the literature 14,15 .…”

Preparation of Chiral Triacylglycerols, <i>sn</i>-POO and <i>sn</i>-OOP, via Lipase-mediated Acidolysis Reaction

“…5 A . Judging from the Snyder s polar parameter 19 value of 0.1, 2.7, and 5.1 for hexane, benzene, and acetone, respectively, these results indicate that polarity of reaction solvents affects the lipase activity for acidolysis reaction. Domingo et al reported that, in lipase-catalyzed aza-Michael addition reactions, the target compounds were successfully synthesized in a non-polar hexane medium, whereas aminolysis side reaction proceeded in polar solvents 20 .…”

“…The mobile phase was a mixture of acetone/acetonitrile 1 : 1, v/ v , and the flow rate was 0.8 mL/min. TAG molecular species were identified using the equivalent carbon number and theoretical carbon number of TAGs, as described in the literature 18,19 . The area ratios of chromatograms were defined as the yields of each TAG generated by the acidolysis reaction.…”

Oxidation Behavior of Triacylglycerol Containing Conjugated Linolenic Acids in sn-1(3) or sn-2 Position

“…Glyceryl tridecanoate was used as the internal standard. Herein, the TAG molecular species were identified using equivalent carbon number ECN 15 and theoretical carbon number TCN 16 of TAGs. For example, the ECN of ODO and tri-CLN-TAG were calculated as 26 and 36, respectively.…”