High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples

Ristivojević, Petar; Tahir, Ammar; Malfent, Fabian; Opsenica, Dušanka Milojković; Rollinger, Judith M.

doi:10.1016/j.chroma.2019.02.006

Cited by 32 publications

(11 citation statements)

References 22 publications

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“…In the case of gallic acid, the results obtained in this study were higher, while for vanillic, caffeic and p -coumaric acids, the results obtained were slightly lower on comparing with the results obtained by Flaczyk et al [31]. This could be explained by the differences in the growing conditions in different regions [32]. Moreover, a literature screening also revealed that the efficiency of phenolic extraction depends on the extraction method, solvent type and drying process used for the plant material [28].…”

Section: Resultscontrasting

confidence: 55%

A Comparative Study on the Phenolic Composition and Biological Activities of Morus alba L. Commercial Samples

et al. 2019

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Morus alba L. (white mulberry) has been commonly used as a functional food and for medicinal purposes. Hence, the aim of the study was to compare the phenolic profile of white mulberry commercial samples in relation to their antioxidant potential and acetylcholinesterase (AChE) inhibitory activity. It is of interest to determine whether herbal products originating from different commercial sources differ in their phenolic profiles. For this purpose, a simple and rapid high-performance liquid chromatography (HPLC) method was used for the separation and determination of ten major phenolic compounds. Total phenolic (TPC), total flavonoid (TFC), and total phenolic acid contents (TPAC), as well as l(+)-ascorbic acid (ASA) contents, were determined. The antioxidant potential was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl radical) scavenging activity and ferric-reducing/antioxidant power (FRAP) assay, while the AChE inhibitory activity was determined by the Ellman assay for water extracts. The study revealed that excluding two herbal products containing fruits and a sample containing leaves of white mulberry, yerba mate and lemon, the remaining samples were generally consistent in terms of phenolic composition as well as antioxidant potential and AChE inhibitory activity. This reflects the health-promoting properties of the samples under study. Moreover, the results showed that the water extracts of white mulberry were richer in phenolic compounds and presented higher antioxidant activity than the hydromethanolic extracts. However, the water extracts showed low inhibitory activity against AChE. Moreover, the correlation analysis indicated a high positive relationship between phenolic composition and antioxidant activity in extracts of white mulberry. Overall, the obtained results may be useful in the evaluation of new dietary supplements and food products. The water extracts of white mulberry could be used for antioxidant purposes, while the hydromethanolic extracts could be incorporated in antioxidant formulations.

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Section: Resultscontrasting

confidence: 55%

A Comparative Study on the Phenolic Composition and Biological Activities of Morus alba L. Commercial Samples

et al. 2019

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“…For visualization, the bioautogram was immersed into a solution of MTT dye with Triton X-100 (1 mg/mL). To enhance the intensity of the yellow bands, one drop of Triton X-100/10 mL MTT aqueous solution was added (Ristivojević et al, 2018;Ristivojević, Tahir, Malfent, Milojkovic, Opsenica & Rollinger, 2019). The obtained bioautograms were documented using TLC Visualizer 2 under white light.…”

Section: Hptlc-bioautography Antimicrobial Assaysmentioning

confidence: 99%

“…After derivatization with DPPH solution (1 mg/mL), the plate was dried for 90 s in the dark at ambient temperature. Then, the obtained bioautogram was documented every 3 min for 30 min using TLC Visualizer 2 under white light (Ristivojević et al, 2018;Ristivojević et al, 2019).…”

Section: Hptlc-bioautography Dpph Assaymentioning

confidence: 99%

Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds

et al. 2021

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Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel highperformance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.

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“…It can not only show the activity of the herbal drugs but also reveal which components contribute to the activity. Due to the merits of being simple, convenient and requiring no laborious isolation, TLC-bioautography has been widely employed for screening and identification of herbal drugs components with bioactivity such as anti-microbial [ 42 ], acetylcholinesterase inhibition [ 43 , 44 ], α- and β-glucosidase inhibition [ 45 , 46 ], free radical scavenging and antioxidation [ 42 , 47 ]. Actually, ChP has already included TLC-bioautography against 2,2-diphenyl-1-picrylhydrazyl radical for the identification of Rehmanniae Radix.…”

Section: Advanced Analytical Techniques For Quality Standard Of Tcmmentioning

confidence: 99%

Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances

et al. 2020

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Traditional Chinese medicine (TCM) are becoming more and more popular all over the world. However, quality issues of TCM may lead to medical incidents in practice and therefore quality control is essential to TCM. In this review, the state of TCM in European Pharmacopoeia are compared with that in Chinese Pharmacopoeia, and herbal drugs that are not considered as TCM and not elaborated by TCM working party at European Directorate for the Quality of Medicines & Health Care (EDQM) but present in both European Pharmacopoeia and Chinese Pharmacopoeias are also discussed. Different aspects in quality control of TCM including origins, identification, tests and assays, as well as sample preparation, marker selection and TCM processing are covered to address the importance of establishing comprehensive quality standard of TCM. Furthermore, advanced analytical techniques for quality control and standard establishment of TCM are also reviewed. Electronic supplementary material The online version of this article (10.1186/s13020-020-00357-3) contains supplementary material, which is available to authorized users.

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High-performance thin-layer chromatography/bioautography and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples

Abstract: High-performance thin-layer chromatography/bioautography and and liquid chromatography-mass spectrometry hyphenated with chemometrics for the quality assessment of Morus alba samples,

Cited by 32 publications

References 22 publications

A Comparative Study on the Phenolic Composition and Biological Activities of Morus alba L. Commercial Samples

A Comparative Study on the Phenolic Composition and Biological Activities of Morus alba L. Commercial Samples

Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds

Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances

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