High peroxidase-like activity realized by facile synthesis of FeS2 nanoparticles for sensitive colorimetric detection of H2O2 and glutathione

Song, Chan; Ding, Wei; Zhao, Weiwen; Liu, Haibo; Wang, Jie; Yao, Yue; Yao, Cheng

doi:10.1016/j.bios.2019.111983

Cited by 160 publications

(76 citation statements)

References 56 publications

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“…In this work, the presence of Cu 2+ ion and Cu + ion in the hybrid nanoflowers was confirmed by the XPS spectrum, and the ratio between Cu 2+ ion to Cu + ion is 1:0.374 ( Figure S2 in Supplementary Information). According to the published reports, several peroxidase mimics generate hydroxyl radical by decomposing H 2 O 2 to accomplish the conversion of TMB to TMB radical cation (oxidized TMB) relying on its peroxidase-like activity (Formula (3)) [ 40 , 41 , 55 ]. Therefore, the amino acid incorporated nanoflowers with intrinsic peroxidase-like activity were chosen as a peroxidase mimic to achieve the oxidation of TMB in this experiment.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, numerous efforts have been devoted to solving these problems by developing novel enzyme mimics to replace the natural enzyme [ 36 ]. To our knowledge, several nanostructures, such as gold nanoclusters, Co 3 O 4 nanoparticles, gold nanoparticles, FeS 2 nanoparticles, or Si doped CoO nanorods possessing instinct peroxidase-like activity were fabricated to detect GSH owing to its greater resistance to adverse surroundings and cost-effectiveness [ 37 , 38 , 39 , 40 , 41 ]. The oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by the mimetic peroxidases can be suppressed by GSH, accompanied by a sharp decrease of the absorbance at 652 nm.…”

Section: Introductionmentioning

confidence: 99%

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The Fabrication of Amino Acid Incorporated Nanoflowers with Intrinsic Peroxidase-like Activity and Its Application for Efficiently Determining Glutathione with TMB Radical Cation as Indicator

Jiang

Zhang

et al. 2021

Micromachines

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The assessment of glutathione (GSH) levels is associated with early diagnostics and pathological analysis for various disorders. Among all kinds of techniques for detecting GSH, the colorimetric assay relying on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by many nanomaterials with peroxidase-like activity attracts increasing attention owing to its outstanding merits, such as high sensitivity and high selectivity. However, the aggregation between the nanomaterials severely hinders the entrance of TMB into the “active site” of these peroxidase mimics. To address this problem, the D-amino acid incorporated nanoflowers possessing peroxidase-like activity with a diameter of 10–15 μm, TMB and H2O2 were employed to establish the detection system for determining the level of glutathione. The larger diameter size of the hybrid nanoflowers substantially averts the aggregation between them. The results confirm that the hybrid nanoflowers detection system presents a low limit of detection, wide linear range, perfect selectivity, good storage stability and desired operational stability for the detection of GSH relying on the intrinsic peroxidase-like activity and favorable mechanical stability of the hybrid nanoflowers, indicating that the hybrid nanoflowers detection system has tremendous application potential in clinical diagnosis and treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Fabrication of Amino Acid Incorporated Nanoflowers with Intrinsic Peroxidase-like Activity and Its Application for Efficiently Determining Glutathione with TMB Radical Cation as Indicator

Jiang

Zhang

et al. 2021

Micromachines

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“…This peroxidase-like activity of mimetic enzymes has been used to colorimetrically detect various bio-related substances such as glucose, 8 hydrogen peroxide (H 2 O 2 ), 9 cysteine 10 and glutathione. 11 However, many mimetic enzymes lack stability and biocompatibility in solution, which restrict their accurate detection of bio-related substances in actual tests. 8 The glucose concentration is closely related to our health and can be detected by using Pd nanoparticles (Pd NPs) based on the colorimetric method.…”

Section: Introductionmentioning

confidence: 99%

Ginkgo biloba leaf polysaccharide stabilized palladium nanoparticles with enhanced peroxidase-like property for the colorimetric detection of glucose

Cui

Lai

et al. 2020

RSC Adv.

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“…In particular, based on the improvement of enzyme catalytic activity, many studies have been carried out to achieve the purpose of colorimetric detection (Zhang et al, 2018 ; Li et al, 2019 ). Besides, most studies have focused on the design of nanozymes with different activities for colorimetric sensing of biothiols (Xiong et al, 2015 ; Song et al, 2020 ), dopamine (Wang et al, 2019b ), glutathione reductase (Zhang et al, 2019 ), and acid phosphatase (Lin et al, 2020 ). These sensors generally achieve the goal of detection by using reduced substances to interfere with the oxidation of chromogenic substrates or to reduce oxTMB.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

et al. 2020

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The impurity of salicylic acid (SA) in aspirin is a required inspection item for drug quality control. Since free SA is significantly toxic for humans, the content determination of free SA is absolutely necessary to ensure people's health. In this work, a facile colorimetric method was developed for the detection of SA in aspirin by utilizing the MIL-53(Fe) nanozyme. As MIL-53(Fe) possesses enzyme mimicking catalytic activity, 3,3,5,5-tetramethylbenzidine (TMB) can be easily oxidized to blue-oxidized TMB (oxTMB) with the existence of H 2 O 2. Moreover, an inhibition effect on the catalytic activity of the MIL-53(Fe) nanozyme is induced due to the specific complexation between SA and Fe 3+ in the center of MIL-53(Fe), which results in a lighter color in the oxTMB. The color change of oxTMB can be seen easily by the naked eye with the addition of different concentrations of SA. Thus, a simple colorimetric platform was established for effectively monitoring SA. A good linear relationship (R 2 = 0.9990) was obtained in the concentration range of 0.4-28 µmol L −1 , and the detection limit was 0.26 µmol L −1. In particular, the rationally designed system has been well-applied to the detection of SA impurity in aspirin. Satisfyingly, the detection results are highly in accord with those of HPLC. This novel colorimetric platform broadens the application prospects of nanozymes in the field of pharmaceutical analysis.

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High peroxidase-like activity realized by facile synthesis of FeS2 nanoparticles for sensitive colorimetric detection of H2O2 and glutathione

Cited by 160 publications

References 56 publications

The Fabrication of Amino Acid Incorporated Nanoflowers with Intrinsic Peroxidase-like Activity and Its Application for Efficiently Determining Glutathione with TMB Radical Cation as Indicator

The Fabrication of Amino Acid Incorporated Nanoflowers with Intrinsic Peroxidase-like Activity and Its Application for Efficiently Determining Glutathione with TMB Radical Cation as Indicator

Ginkgo biloba leaf polysaccharide stabilized palladium nanoparticles with enhanced peroxidase-like property for the colorimetric detection of glucose

Colorimetric Detection of Salicylic Acid in Aspirin Using MIL-53(Fe) Nanozyme

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