High plasma adenosine levels in overweight/obese pregnant women

Abstract

We aim to investigate whether overweight/obese pregnant women have elevated plasma levels of adenosine associated with increased consumption of high-calorie food. Sixty women were included. They were divided into lean (n = 23 and n = 12) or overweight/obese (n = 7 and n = 18) non-pregnant and pregnant women, respectively. Clinical records and maternal blood samples were collected after informed consent. A self-reported dietary questionnaire was also completed. Plasma adenosine levels were determined with high-performance liquid chromatography. Biochemical parameters, including glucose, total protein, and lipid profile, were determined using standard colorimetric assays. Adenosine levels were higher in pregnant women than in non-pregnant women (18.7 ± 1.6 vs 10.8 ± 1.3 nM/μg protein, respectively, p < 0.0001). Overweight/obese pregnant women (21.9 ± 2.5 nM/μg protein) exhibited higher adenosine levels than lean pregnant (14.5 ± 1.0 nM/μg protein, p = 0.04) or non-pregnant women (11.7 ± 1.5 nM/μg protein, p = 0.0005). Also, pregnant women with elevated weight gain exhibited higher (26.2 ± 3.7 nM/μg protein) adenosine levels than those with adequate weight gain (14.9 ± 1.4 nM/μg protein, p = 0.03). These differences were not statistically significant compared with those of pregnant women with reduced weight gain (17.4 ± 2.1 nM/μg protein, p = 0.053). Body mass index and adenosine only in pregnant women were positively correlated (r = 0.39, p = 0.02). While, polyunsaturated fatty acid (PUFA) consumption was negatively correlated with plasma adenosine levels only in non-pregnant women (r = -0.33, p = 0.03). Pregnancy is associated with high plasma adenosine levels, which are further elevated in pregnant women who are overweight/obese. High PUFA intake might reduce plasma adenosine levels in non-pregnant women.

Blueberry intake included in hypocaloric diet decreases weight, glucose, cholesterol, triglycerides and adenosine levels in obese subjects

“…Literature suggests that blueberries consumption has been associated with amelioration of obesity as well as diminution of obesity-related pro-inflammatory molecules (Badillo et al, 2017;Burnstock & Gentile, 2018;Cáceres-Medina et al, 2015;Margalef et al, 2017;Noratto et al, 2016;Oviedo-Solís, Cornejo-Manzo, & Murillo-Ortiz, 2018;Pandolfi et al, 2015;Wyckoff, Evans, Manasse, Butryn, & Forman, 2017). Thus, we included blueberries as beneficial health-promoting food in hypocaloric diet as suggested by others (Wu, Yang, et al, 2018;Zhang, Ma, Luo, & Li, 2018).…”

Lack of adipocyte purinergic P2Y 6 receptor greatly improves whole body glucose homeostasis

“…These agents play key roles in maintaining many important metabolic functions. High plasma levels of nucleoside/nucleotides such as adenosine and uridine have been reported under conditions of obesity, acting on G protein-coupled adenosine and P2Y receptors (P2YRs) of the purinergic receptor family (11,12). P2Y receptors such as P2Y 1 R and P2Y 14 R have been shown to play a significant role in the regulation of leptin secretion and insulin sensitivity (13,14).…”

The involvement of purinergic signalling in obesity

“…A review about adenosine and adipogenesis is available [121]. High plasma levels of adenosine were found in children with obesity [122] and in overweight pregnant women [123]. Non-alcoholic fatty liver disease is an obesity-related condition.…”