High Potential Iron Sulfur Proteins

Abstract

Functional Class Occurrence Biological Function Amino Acid Sequence Information Protein Production, Purification and Molecular Characterization Metal Content and Cofactors Spectroscopy Activity Tests X ‐Ray Structures of Native Hi PIP Model Fe 4 S 4 Complexes Functional Aspects Electron Transfer Mechanisms Acknowledgments Related Articles

Detailed assessment of X-ray induced structural perturbation in a crystalline state protein

“…High-potential iron-sulfur protein (HiPIP) 1 acts as a soluble electron carrier of the electron transfer reaction in photosynthetic Gram negative bacteria (Carter, 2001). The molecular mass of the holoprotein is 9.1 kDa (Moulis et al, 1993).…”

Ultra–high‐resolution structure and charge‐density analysis of high‐potential iron–sulfur protein

“…The cuboidal Fe 4 S 4 cluster is one of the most common among them [2]. This cluster provides electron-conducting and -storing components in electron transfer proteins such as highpotential iron-sulfur protein (HiPIP) [3], ferredoxin [4], and photosystem I [5]. It also serves as an active site in enzymes such as aconitase [6] and quinolinate synthase [7].…”

“…HiPIP is a soluble electron transfer protein involved in bacterial photosynthesis. HiPIP consists of~80 residues and possesses one Fe 4 S 4 cluster, and its typical redox potential is~+350 mV [3]. Several HiPIP structures have been determined by X-ray crystallography.…”

“…Several HiPIP structures have been determined by X-ray crystallography. HiPIP from the mesophilic purple bacterium Allochromatium vinosum has been the most intensively studied homolog, and a variety of methods have been used for this purpose [3,9]. On the other hand, we have performed crystallographic analyses of HiPIP from the thermophilic purple bacterium Thermochromatium Abbreviations AIM, atoms in molecules; HiPIP, high-potential iron-sulfur protein; ISAM, independent spherical atom model; MAM, multipolar atomic model.…”