High precision dual-modulation differential terahertz ATR sensor for liquid measurements

Zheng, Xiujun; Gevart, Thomas; Gallot, Guilhem

doi:10.1364/ol.430324

Cited by 9 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting reflected terahertz signal is then correlated with the cytosol content [2][3][4], in real time without any marker or specific sample preparation. In our experiments, we used a differential setup based on a QCL source to obtain stable and precise measurements [5].…”

Section: Methodsmentioning

confidence: 99%

Terahertz ATR sheds light on real-time exchange kinetics occurring through plasma membrane during photodynamic therapy

Lordon,

Zheng,

Mingotaud

et al. 2023

EPJ Web Conf.

View full text Add to dashboard Cite

THz ATR spectroscopy provides, in a single measurement, the relative number of defects per membrane surface created by oxidative stress generated during photodynamic therapy (PDT), offering early, sensitive real-time information. THz spectroscopy is therefore a complementary technique to established (biological) assays and can be applied to any topic requiring the real-time examination of short-term plasma membrane permeabilization.

show abstract

Section: Methodsmentioning

confidence: 99%

Terahertz ATR sheds light on real-time exchange kinetics occurring through plasma membrane during photodynamic therapy

Lordon,

Zheng,

Mingotaud

et al. 2023

EPJ Web Conf.

View full text Add to dashboard Cite

show abstract

“…Our THz sensor is a differential ATR setup with a capacity to detect a relative reflectivity variation of 10 −4 , corresponding to a micromolar level of detection of molecules in solution. THz attenuated total reflection (THz-ATR) makes use of the evanescent wave at the back of a prism under total internal reflection, [41,42] which is coupled to the sample under study (Figure 4 and Figure S5, Supporting Information). Providing that the thickness of the cell layer matches the penetration depth of the evanescent wave, the reflected THz wave is modified by the THz relative permittivity value and repartition in the cell layer in contact with the top of the prism.…”

Section: Permeabilization Kinetics Examined By Thz Spectroscopymentioning

confidence: 99%

Terahertz Spectroscopy Sheds Light on Real‐Time Exchange Kinetics Occurring through Plasma Membrane during Photodynamic Therapy Treatment

et al. 2023

Self Cite

View full text Add to dashboard Cite

Methods to follow in real time complex processes occurring along living cell membranes such as cell permeabilization are rare. Here, the terahertz spectroscopy reveals early events in plasma membrane alteration generated during photodynamic therapy (PDT) protocol, events which are not observable in any other conventional biological techniques performed in parallel as comparison. Photodynamic process is examined in Madin-Darby canine kidney cells using Pheophorbide (Pheo) photosensitizer alone or alternatively encapsulated in poly(ethylene oxide)-block-poly(𝝐-caprolactone) micelles for drug delivery purpose. Terahertz spectroscopy (THz) reveals that plasma membrane permeabilization starts simultaneously with illumination and is stronger when photosensitizer is encapsulated. In parallel, the exchange of biological species is assessed. Over several hours, this conventional approach demonstrates significant differences between free and encapsulated Pheo, the latter leading to high penetration of propidium iodide, Na + and Ca 2+ ions, and a high level of leakage of K + , ATP, and lactate dehydrogenase. THz spectroscopy provides, in a single measurement, the relative number of defects per membrane surface created after PDT, which is not achieved by any other method, providing early, sensitive real-time information. THz spectroscopy is therefore a promising technique and can be applied to any biological topic requiring the examination of short-term plasma membrane permeabilization.

show abstract

“…The direct consequence is that it requires keeping a temperature stability below 0.1°C. On the bright side, the strong absorption of water and its variation havebeen exploited to better understand the relationship between the shape of a protein and the solvent dynamics around it [23,24,25], and to better understand the cell membrane [26,27,28,29].…”

Section: Thz Spectroscopy For Biology and Medicinementioning

confidence: 99%

Terahertz technology for biophotonics: a general overview and recent advances in THz spectroscopy toward biology

Peretti

2023

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI

View full text Add to dashboard Cite

In this conference, we will discuss the advancements in Terahertz (THz) spectroscopy and imaging regarding biophotonics applications. In recent years, THz technology helped various fields, from fundamental physics to industrial process control. THz spectrometers offer a wide spectrum range and high dynamic range, enabling the analysis of semi-transparent materials and living tissues. THz imaging has shown potential in applications such as cancer detection, diabetes foot syndrome, burn wound analysis, and plant hydration assessment. Spectroscopy in the THz range is particularly promising for studying biological systems, including proteins and their compounds. However, challenges such as the strong absorption of water, temperature stability, and small sample sizes need to be overcome. Techniques like scanning microscopy, integrated photonics, and metallic broadband approaches have been explored to enhance THz analysis. Despite the challenges, THz technology has found industrial applications and efforts are being made to improve data processing and error evaluation. Additionally, a proposed method aims to enhance the resolution of THz systems, making them suitable for gas spectroscopy and the sensing of volatile organic compounds relevant to biology.

show abstract

High precision dual-modulation differential terahertz ATR sensor for liquid measurements

Cited by 9 publications

References 25 publications

Terahertz ATR sheds light on real-time exchange kinetics occurring through plasma membrane during photodynamic therapy

Terahertz ATR sheds light on real-time exchange kinetics occurring through plasma membrane during photodynamic therapy

Terahertz Spectroscopy Sheds Light on Real‐Time Exchange Kinetics Occurring through Plasma Membrane during Photodynamic Therapy Treatment

Terahertz technology for biophotonics: a general overview and recent advances in THz spectroscopy toward biology

Contact Info

Product

Resources

About