High pressure-assisted encapsulation of vitamin D₂in reassembled casein micelles

Abstract

For the encapsulation of vitamin D 2 , native casein micelles and vitamin D 2 with or without additional Ca 2+ -P i were treated at 600 MPa and 37 • C for 60 min. The pressure release rate was set at 20 or 600 MPa/min. Vitamin D 2 was quantified by reversed-phase high-performance liquid chromatography, and physical properties of the micelles were analysed by photon correlation spectroscopy. The results demonstrate that simultaneous application of Ca 2+ -P i and high pressure treatment with a fast release rate significantly increased loading of vitamin D 2 per casein by 6.9-fold. The addition of Ca 2+ -P i enhanced micelle aggregation and the vitamin was entrapped within the formed aggregates. However, high pressure treatment without Ca 2+ -P i with a slow pressure release rate revealed similar results, increasing vitamin D 2 per casein by 6.7-fold. The vitamin D 2 loading in reassembled casein micelles is supposed to be due to hydrophobic interactions between the hydrophobic domains of the micelles.

Native casein micelles as nanocarriers for β‐carotene: pH‐and temperature‐induced opening of the micellar structure

“…Menendez‐Aguirre et al . () encapsulated vitamin D2 in native CM that were isolated via microfiltration, subsequently spray dried and then disintegrated by high hydrostatic pressure (Post et al ., ; Menendez‐Aguirre et al ., ). However, it cannot be excluded that the structure of CM might be changed as a result of technological processes.…”

“…Although CM already have a semi-open structure, they must be further treated to make the hydrophobic core accessible for the lipophilic compounds. Menendez-Aguirre et al (2011) encapsulated vitamin D2 in native CM that were isolated via microfiltration, subsequently spray dried and then disintegrated by high hydrostatic pressure (Post et al, 2009;Menendez-Aguirre et al, 2011). However, it cannot be excluded that the structure of CM might be changed as a result of technological processes.…”

Encapsulation of cholecalciferol and ergocalciferol in oil‐in‐water emulsions by different homogenization techniques

“…In general, each delivery system must be specifically designed for each application depending on the characteristics of the active agent to be encapsulated, e.g., solubility, physical, and chemical stability. Vitamin D has been encapsulated in numerous systems such as nanoemulsions , nanoparticles , casein micelles , and Poly(D, L)Lactic acid (PLA) nanoparticles prepared from nanoprecipitation method .…”

Modifications of structures and functions of caseins: a scientific and technological challenge

“…The gel strength increased with increasing high-pressure treatment (Johnston et al 1992;Desobry-Banon et al 1994) and a syneresis resistance was observed. Highpressure treatments appear as an interesting tool to entrap different ligands like curcumin, vitamin D 2 , and α-tocopherol (Benzaria et al 2013;Menéndez-Aguirre et al 2011;Chevalier-Lucia et al 2011). It increases the binding constant of these compounds to casein micelles.…”