1982
DOI: 10.1093/jaoac/65.2.256
|View full text |Cite
|
Sign up to set email alerts
|

High Pressure Liquid Chromatographic Determination of Monoand Disaccharides in Presweetened Cereals: Collaborative Study

Abstract: A collaborative study was conducted using a modified AOAC method (sugars in chocolate) for the determination of fructose, glucose, sucrose, and maltose in presweetened cereals by high pressure liquid chromatography (HPLC). Eight samples consisting of 6 products were analyzed in duplicate by the HPLC method and the AOAC Lane-Eynon method. The AOAC method was modified to use water-alcohol (1 + 1) and Sep-Pak C18 cartridges for sample cleanup. The HPLC results indicate precision comparable to the Lane-Eynon metho… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
10
0

Year Published

1989
1989
2020
2020

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 16 publications
(10 citation statements)
references
References 0 publications
0
10
0
Order By: Relevance
“…The moisture and ash (925.10; 945.38), crude protein (925.31) and lipid (963.15) content were determined in accordance with AACC standard methods [ 33 ]. Carbohydrates were evaluated as total starch [ 34 ] and simple sugars [ 35 ]; the starch fraction was also characterized in terms of amylose content by means of a commercial kit (K-AMYL, Megazyme Int., Wicklow, Ireland). Soluble and insoluble dietary fibre was assessed by the enzymatic gravimetric procedure [ 36 ].…”
Section: Methodsmentioning
confidence: 99%
“…The moisture and ash (925.10; 945.38), crude protein (925.31) and lipid (963.15) content were determined in accordance with AACC standard methods [ 33 ]. Carbohydrates were evaluated as total starch [ 34 ] and simple sugars [ 35 ]; the starch fraction was also characterized in terms of amylose content by means of a commercial kit (K-AMYL, Megazyme Int., Wicklow, Ireland). Soluble and insoluble dietary fibre was assessed by the enzymatic gravimetric procedure [ 36 ].…”
Section: Methodsmentioning
confidence: 99%
“…Sugars were assessed by high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPAEC-PAD) (Rocklin and Pohl 1983). Briefly, 1 g of the sample was extracted with 200 mL of distilled water at 60°C for 60 min (Zygmunt et al 1982); the extract solution was analyzed with an HPAEC-PAD equipped with a CarboPac PA1 (4 × 250 mm) column plus a CarboPac PA1 (4 × 50 mm) guard column (Dionex, Sunnyvale, CA, U.S.A.) and an ED50 pulsed amperometric detector (Dionex). Starch content was calculated by difference (100 -amount of all the other chemical components).…”
Section: Molecular Characterization Of Lab Strains By Randomlymentioning
confidence: 99%
“…Centrifuge 5 minute at 3500 rpm and filter through 0.45-0.7 µm. The filtrate was used to determine the sugar content in solid waste (Zygmunt, 1982).…”
Section: Materials and Metods Substratementioning
confidence: 99%