High pressure liquid chromatography and widebore capillary gas-liquid chromatography methods for quantification of acetoin and diacetyl from bacterial cultures

Montville, Thomas J.; Meyer, Mary Elizabeth; Hsu, Amy; Huang, George Tzou-Chi

doi:10.1016/0167-7012(87)90002-9

Cited by 18 publications

(8 citation statements)

References 11 publications

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“…End products were quantified by high-pressure liquid chromatography (23). In some instances, lactate concentrations were also determined enzymatically by monitoring the increase in NAD A340 (Sigma Chemical Co., St. Louis, Mo.…”

Section: Methodsmentioning

confidence: 99%

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O ₂

Tseng

Montville

1990

Appl Environ Microbiol

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Lactobacillus plantarum catabolic end products changed in response to environmental conditions. While lactate was always the major end product, acetate was produced in alkaline and aerobic environments. Acetoin levels decreased under alkaline conditions. Changes in acetoin dehydrogenase, acetate kinase, NADH oxidase, pyruvate oxidase, and acetate kinase activities correlated with changes in end product distribution.

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Section: Methodsmentioning

confidence: 99%

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O ₂

Tseng

Montville

1990

Appl Environ Microbiol

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“…Cells for pyruvate transport studies were grown in a modification (Montville et al, 1987c) of the yeast extract mineral salts medium described by Craig and Snell (1951), designated CST. After growth at 35°C for 24 hr in CST, the stationary phase cells were washed, collected by centrifugation and resuspended in sterile physiological saline for use in pyruvate utilization studies.…”

Section: Methodsmentioning

confidence: 99%

Relationship between pyruvate utilization and acetoin production byLactobacillus plantarum:influence of carbon source, pyruvate concentration, and metabolic inhibitors

Tsau

Montvllle

1990

Food Biotechnology

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The relationship between pyruvate utilization and acetoin production was elucidated by incubating Lactobacillus plantarum cells in buffered reaction mixtures which contained varying concentrations of pyruvate, glucose, and metabolic inhibitors.Pyruvate utilization was greatest at pH 4.5, was pseudo-first order with regard to pyruvate concentration, and was stimulated by glucose. Fructose, galactose, sucrose and lactose were also stimulatory, but maltose was not. The molar conversion efficiency for pyruvate to acetoin was 72-84% at pH 4.5, but only 37-45% at pH 5.5. Pyruvate utilization decreased when the metabolic inhibitors N,N' dicyclohexylcarbodiimide, carbonylcyanide m-chlorophenylhydrazone or 2,4 dinitrophenol were added to the buffers. These data suggests that energy, possibly in the form of proton motive force, is required for pyruvate utilization in L. plantarum.

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“…Fermentation end products were quantified by high pressure liquid chromatography (Montville et al 1987b). Fermentation end products were quantified by high pressure liquid chromatography (Montville et al 1987b).…”

Section: Preparation Of Cell-free Extracts At the Time Intervals Shomentioning

confidence: 99%

Enzymatic regulation of glucose catabolism by Lactobacillus plantarum in response to pH shifts in a chemostat

Tseng

Montville

1992

Appl Microbiol Biotechnol

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The influence of medium pH on the regulation of glucose catabolism by Lactobacillus plantarum 8014 was examined in anaerobic chemostat cultures.When L. plantarum was grown in a chemostat at pH 5.5, and the pH shifted to pH 7.5, acetate was produced in addition to lactate and acetoin. After the shift, acetate kinase and NAD-dependent lactate dehydrogenase activities increased while the acetoin dehydrogenase and alpha-acetolactate synthase activities decreased. The high acetate kinase activity together with low acetoin dehydrogenase and alpha-acetolactate synthase activities may explain why L. plantarum made more acetate at the expense of acetoin in response to alkaline conditions.

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High pressure liquid chromatography and widebore capillary gas-liquid chromatography methods for quantification of acetoin and diacetyl from bacterial cultures

Cited by 18 publications

References 11 publications

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O ₂

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O ₂

Relationship between pyruvate utilization and acetoin production byLactobacillus plantarum:influence of carbon source, pyruvate concentration, and metabolic inhibitors

Enzymatic regulation of glucose catabolism by Lactobacillus plantarum in response to pH shifts in a chemostat

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High pressure liquid chromatography and widebore capillary gas-liquid chromatography methods for quantification of acetoin and diacetyl from bacterial cultures

Cited by 18 publications

References 11 publications

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O 2

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O 2

Relationship between pyruvate utilization and acetoin production byLactobacillus plantarum:influence of carbon source, pyruvate concentration, and metabolic inhibitors

Enzymatic regulation of glucose catabolism by Lactobacillus plantarum in response to pH shifts in a chemostat

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Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O ₂

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O ₂