High-purity Hepatic Lineage Differentiated from Dental Pulp Stem Cells in Serum-free Medium

Ishkitiev, Nikolay; Yaegaki, Ken; Imai, Toshio; Tanaka, Tomoko; Nakahara, Taka; Ishikawa, Hiroshi; Mitev, Vanio; Haapasalo, Markus

doi:10.1016/j.joen.2011.12.011

Cited by 91 publications

(79 citation statements)

References 34 publications

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“…DPSCs are well known to be multi-potent cells capable of differentiation along multiple lineages [1]. DPSCs have the remarkable potential for multi-lineage differentiation capacity including osteoblast [2], cartilage [3], adipocyte [4], muscle [5], hepatocyte [6], and neurons [7]. Therefore, an improved comprehension of the cellular and molecular mechanisms, which modulate self-renewal and differentiation properties of DPSCs, could be pursued to bring forth future progress in regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

Concurrent Expression of Oct4 and Nanog Maintains Mesenchymal Stem-Like Property of Human Dental Pulp Cells

Huang

Chen

et al. 2014

IJMS

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Human dental pulp stem cells (DPSCs), unique mesenchymal stem cells (MSCs) type, exhibit the characteristics of self-renewal and multi-lineage differentiation capacity. Oct4 and Nanog are pluripotent genes. The aim of this study was to determine the physiological functions of Oct4 and Nanog expression in DPSCs. Herein, we determined the critical role of an Oct4/Nanog axis modulating MSCs properties of DPSCs by lentiviral-mediated co-overexpression or co-knockdown of Oct4/Nanog in DPSCs. MSCs properties including osteogenic/chondrogenic/adipogenic induction differentiation was assayed for expression of osteogenic/chondrogenic/adipogenic markers by quantitative real-time RT-PCR analysis. Initially, we observed that the expression profile of Oct4 and Nanog in dental pulp cells, which exerted properties of MSCs, was significantly up-regulated compared to that of STRO-1−CD146− dental pulp cells. Down-regulation of Oct4 and Nanog co-expression significantly reduced the cell proliferation, osteogenic differentiation capability, STRO-1, CD146, and Alkaline phosphatase (ALP) activity of DPSCs. In contrast, co-overexpression of Oct4 and Nanog enhanced the expression level of STRO-1 and CD146, proliferation rate and osteogenic/chondrogenic/adipogenic induction differentiation capability, and expression of osteogenic/chondrogenic/adipogenic induction differentiation markers. Our results suggest that Oct4-Nanog signaling is a regulatory switch to maintain properties in DPSCs.

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Section: Introductionmentioning

confidence: 99%

Concurrent Expression of Oct4 and Nanog Maintains Mesenchymal Stem-Like Property of Human Dental Pulp Cells

Huang

Chen

et al. 2014

IJMS

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“…We have previously shown that these cells under appropriate cue can be differentiated into beta-like cells (Govindasamy et al 2010a), adipocytes (Govindasamy et al 2010b), chondrocytes and osteocytes (Govindasamy et al 2010a). Likewise, others also have reported the ability of dental pulp stem cells from deciduous teeth (SHED) into cardiac-like (Samper et al 2013) and hepatic like cells (Ishkitiev et al 2012). As such, SHED are now being investigated as closely relevant cell source to tackle issues related to neurons owing to their inborn ectodermal origin.…”

Section: Introductionmentioning

confidence: 92%

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

Gnanasegaran

Govindasamy²,

Musa

et al. 2014

Cytotechnology

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Among the debilitating diseases, neurological related diseases are the most challenging ones to be treated using cell replacement therapies. Recently, dental pulp stem cells (SHED) were found to be most suitable cell choice for neurological related diseases as evidenced with many preclinical studies. To enhance the neurological potential of SHED, we recapitulated one of the pharmacological therapeutic tools in cell replacement treatment, we pre-conditioned dental pulp stem cells (SHED) with culture medium of ReNCell VM, an immortalized neuron progenitor cell, prior to neurogenesis induction and investigated whether this practice enhances their neurogenesis potential especially towards dopaminergic neurons. We hypothesed that the integration of pharmacological practices such as co-administration of various drugs, a wide range of doses and duration as well as pre-conditioning into cell replacement may enhance the efficacy of stem cell therapy. In particular, pre-conditioning is shown to be involved in the protective effect from some membrano-tropic drugs, thereby improving the resistance of cell structures and homing capabilities. We found that cells pre-treated with ReNCell VM conditioned medium displayed bipolar structures with extensive branches resembling putative dopaminergic neurons as compared to nontreated cells. Furthermore, many neuronal related markers such as NES, NR4A2, MSI1, and TH were highly expressed (fold changes [ 2; p \ 0.05) in pretreated cells. Similar observations were detected at the protein level. The results demonstrate for the first time that SHED pre-conditioning enhances neurological potential and we suggest that cells should be primed to their respective environment prior to transplantation.

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“…Human dental pulp stem cells (hDPSCs) are observed to differentiate into hepatocyte-like cells (Ishkitiev et al 2012). Because of this, a recent study tested the benefits of this treatment and melatonin (5 mg/kg i.p.…”

Section: Liver Transplantationmentioning

confidence: 99%

Potential benefits of melatonin in organ transplantation: a review

Esteban‐Zubero

García-Gil

López-Pingarrón

et al. 2016

Journal of Endocrinology

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Organ transplantation is a useful therapeutic tool for patients with end-stage organ failure; however, graft rejection is a major obstacle in terms of a successful treatment. Rejection is usually a consequence of a complex immunological and nonimmunological antigen-independent cascade of events, including free radical-mediated ischemia-reperfusion injury (IRI). To reduce the frequency of this outcome, continuing improvements in the efficacy of antirejection drugs are a top priority to enhance the long-term survival of transplant recipients. Melatonin (N-acetyl-5-methoxytryptamine) is a powerful antioxidant and ant-inflammatory agent synthesized from the essential amino acidl-tryptophan; it is produced by the pineal gland as well as by many other organs including ovary, testes, bone marrow, gut, placenta, and liver. Melatonin has proven to be a potentially useful therapeutic tool in the reduction of graft rejection. Its benefits are based on its direct actions as a free radical scavenger as well as its indirect antioxidative actions in the stimulation of the cellular antioxidant defense system. Moreover, it has significant anti-inflammatory activity. Melatonin has been found to improve the beneficial effects of preservation fluids when they are enriched with the indoleamine. This article reviews the experimental evidence that melatonin is useful in reducing graft failure, especially in cardiac, bone, otolaryngology, ovarian, testicular, lung, pancreas, kidney, and liver transplantation.

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High-purity Hepatic Lineage Differentiated from Dental Pulp Stem Cells in Serum-free Medium

Cited by 91 publications

References 34 publications

Concurrent Expression of Oct4 and Nanog Maintains Mesenchymal Stem-Like Property of Human Dental Pulp Cells

Concurrent Expression of Oct4 and Nanog Maintains Mesenchymal Stem-Like Property of Human Dental Pulp Cells

ReNCell VM conditioned medium enhances the induction of dental pulp stem cells into dopaminergic like cells

Potential benefits of melatonin in organ transplantation: a review

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