High-Quality Draft Genome Sequence of Pseudomonas reidholzensis Strain CCOS 865 ^T

Abstract

We have sequenced and assembled the genome of Pseudomonas reidholzensis CCOS 865T, which was isolated in 2014 from forest soil. Members of the genus Pseudomonas play important roles in environmental systems and are utilized in many biotechnological processes.

Comparative Genomic Analysis of the Biotechnological Potential of the Novel Species Pseudomonas wadenswilerensis CCOS 864T and Pseudomonas reidholzensis CCOS 865T

“…The genome sizes of 5 966 942 bp for P. wadenswilerensis CCOS 864 T [17] and 6 163 129 bp for P. reidholzensis CCOS 865 T [18] were well within the range of the minimum (4 655 082 bp) and maximum (6 663 130 bp) of other members of the P. putida group. A total number of 5 437 genes for P. wadenswilerensis CCOS 864 T and 5 441 genes for P. reidholzensis CCOS 865 T were annotated.…”

“…In order to perform a pan-genome analysis to identify genomic features that differ between the genomes of either P. wadenswilerensis CCOS 864 T [17] or P. reidholzensis CCOS 865 T [18], a total of 123 genome sequences were selected from GenBank. The selection criteria were: a) allocation to a species known to belong to the P. putida group; and b) covering the species with as many as possible genomes to allow statements on species-specific traits.…”

The importance of genome sequence quality to microbial comparative genomics

“…The above mentioned process often yields closure of plasmid sequences from draft genomes [18], but also routinely a reduction of the total number of contigs to under 50 contigs per genome [19–21] with near complete removal of small contigs. Due to a thorough quality check at every assembly step by repeated read mapping and visual checking (Fig.…”