High-Resolution Chromosome Preparations for G- and R-Banding in Gallus domesticus

Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH

“…The banding patterns of the eight pairs of autosomal macrochromosomes and ZW chromosomes were analyzed in 17 metaphase chromosome spreads. Only 5 (1.6 %) of the 306 chromosomes observed exhibited ambiguous or unusual R-band patterns, in comparison with published karyotypes (Ponce de Leó n et al, 1992;Ladjali et al, 1995). These anomalous bands included unclear bands, darkly stained minor bands, lightly stained major bands, and indistinguishable chro- Fig.…”

“…It has allowed rapid and efficient construction of a high-density, highresolution cytogenetic map of DNA clones in human chromosomes Takahashi et al, 1992). A method for obtaining replication R-banded chromosomes has been reported in chicken fibroblast cell cultures (Ponce de Leó n et al, 1992;Ladjali et al, 1995) and has subsequently been used in combination with FISH for cytogenetic mapping (Ambady et al, 1997). However, this technique has not been systematically used to construct a chromosome map of functional genes in the chicken.…”

International System for Standardized Avian Karyotypes (ISSAK): standardized banded karyotypes of the domestic fowl (Gallus domesticus)

“…Cultures were synchronized as described by Ladjali et al (1995) with a double thymidine block during S phase in order to increase the yield of metaphase and early metaphase cells. To obtain the RBG-FPG bands (R-bands obtained incorporating BrdU followed by fluorochrome-photolysis and Giemsa stain), BrdU (Sigma; final concentration 10 Ìg/ml) was added to the cell culture as previously described (Romagnano and Richer 1984;ViegasPéquignot et al, 1989;Schmid et al, 1989;Ladjali et al, 1995). The slides were incubated in Hoechst 33258 (1 mg/100 ml) for 15 to 20 min at room temperature.…”

First report on chicken genes and chromosomes 2000

“…8) (Ladjali-Mohammedi et al, 1999). Considering the chicken standard karyotype as a reference (Carlenius et al, 1981;Ponce de Leon et al, 1992;Ladjali et al, 1995;Ladjali-Mohammedi et al, 1999), ideograms based on banding patterns previously described have been established for the duck (Anas platyrhynchos) (Mayr et al, 1989a;Denjean et al, 1996), the quail (Coturnix coturnix) (Stock and Bunch, 1982;Mayr et al, 1989b;Schmid et al, 1989;Suzuki et al, 1999c) and the turkey (Meleagris gallopavo) Tegelström, 1981, 1983;Stock and Bunch, 1982). All chromosomes have been classified by decreasing size (Figs.…”