High‐resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography <i>in vivo</i>

Zhang, Pengfei; Li, Lei; Lin, Li; Hu, Peng; Shi, Junhui; He, Yun; Zhu, Linyu; Zhou, Yong; Wang, Lihong V.

doi:10.1002/jbio.201700024

Cited by 108 publications

(87 citation statements)

References 28 publications

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“…Many practical implementations of PACT prefer handheld transducers [9,10]. We also demonstrated the feasibility of DS-PACT with a handheld linear-array-based PACT system (Fig.…”

Section: Resultsmentioning

confidence: 60%

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Dichroism-sensitive photoacoustic computed tomography

Qu¹,

Li²,

Shen³

et al. 2018

Optica

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Photoacoustic computed tomography (PACT), a fast-developing modality for deep tissue imaging, images the spatial distribution of optical absorption. PACT usually treats the absorption coefficient as a scalar. However, the absorption coefficients of many biological tissues exhibit an anisotropic property, known as dichroism or diattenuation, which depends on molecular conformation and structural alignment. Here, we present a novel imaging method called dichroism-sensitive PACT (DS-PACT), which measures both the amplitude of tissue's dichroism and the orientation of the optic axis of uniaxial dichroic tissue. By modulating the polarization of linearly polarized light and measuring the alternating signals through lock-in detection, DS-PACT can boost dichroic signals from biological tissues. To validate the proposed approach, we experimentally demonstrated the performance of DS-PACT by imaging plastic polarizers and ex vivo bovine tendons deep inside scattering media. We successfully detected the orientation of the optic axis of uniaxial dichroic materials, even at a depth of 4.5 transport mean free paths. We anticipate that the proposed method will extend the capability of PACT to imaging tissue absorption anisotropy.

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“…Many practical implementations of PACT prefer handheld transducers [9,10]. We also demonstrated the feasibility of DS-PACT with a handheld linear-array-based PACT system (Fig.…”

Section: Resultsmentioning

confidence: 60%

“…PA computed tomography (PACT) is an embodiment of PAT that provides up to multiple centimeters' imaging depth in tissues with high spatial resolution [8][9][10][11]. Here, we present a new approach, called dichroism-sensitive PACT (DS-PACT), which we developed by upgrading a conventional PACT system [12] for dichroism measurement.…”

Section: Introductionmentioning

confidence: 99%

Dichroism-sensitive photoacoustic computed tomography

Qu¹,

Li²,

Shen³

et al. 2018

Optica

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“…It combines the rich optical contrast with high ultrasound resolution [23][24][25][26][27]. Therefore, a non-invasive, safe and better imaging alternative is required.…”

Section: Introductionmentioning

confidence: 99%

Hand‐held, clinical dual mode ultrasound ‐ photoacoustic imaging of rat urinary bladder and its applications

Sivasubramanian

Periyasamy

Dienzo

et al. 2018

Journal of Biophotonics

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Urinary bladder imaging is critical to diagnose urinary tract disorders, and bladder cancer. There is a great need for safe, non-invasive, and sensitive imaging technique which enables bladder imaging. Photoacoustic imaging is a rapidly growing imaging technique for various biological applications. It can be combined with clinical ultrasound imaging system for hand-held, dual modal ultrasound-photoacoustic real-time imaging. Structural (bladder wall) and functional (accretion of nanoparticles) bladder imaging is shown here with combined ultrasound and photoacoustic imaging in rats. Photoacoustic images of bladder wall is shown using black ink as the contrast agent. Chicken tissues were stacked on the abdomen of the animal to demonstrate the feasibility of photoacoustic imaging till a depth of 2 cm. Also, the feasibility of photoacoustic imaging for a common bladder disorder, vesicoureteral reflux is studied using urinary tract mimicking phantom. It is also shown that a clinical ultrasound system can be used for photoacoustic imaging of non-invasive clearance study of gold nanorods from circulation by monitoring the gradual accumulation of the gold nanorods in the bladder. The time taken for accumulation of nanorods in the bladder can be used as an indicator of the clearance rate of the nanoparticle circulation from the body.

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“…26,28,29 In linear-array-based PACT, a common choice for the Hilbert transformation direction is the array receiving direction (the depth direction) in the reconstructed images, taking the absolute value extracts the envelope information of images. 12,30 However, in FR-PACT, the acoustic signals are received from all directions, up to an angle of 2π in plane, which makes the determination of the transformation direction difficult. To address this issue, we explored multiple ways of implementing Hilbert transformation in FR-PACT (in the Appendix).…”

Section: Introductionmentioning

confidence: 99%

Multiview Hilbert transformation in full-ring transducer array-based photoacoustic computed tomography

Zhu

Shen

et al. 2017

J. Biomed. Opt

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transformation in full-ring transducer array-based photoacoustic computed tomography," J. Biomed. Opt. 22(7), 076017 (2017), doi: 10.1117/1.JBO.22.7.076017. Abstract. Based on the photoacoustic (PA) effect, PA tomography directly measures specific optical absorption, i.e., absorbed optical energy per unit volume. We recently developed a full-ring ultrasonic transducer arraybased photoacoustic computed tomography (PACT) system for small-animal whole-body imaging. The system has a full-view detection angle and high in-plane resolution (∼100 μm). However, due to the bandpass frequency response of the piezoelectric transducer elements and the limited elevational detection coverage of the full-ring transducer array, the reconstructed images present bipolar (i.e., both positive and negative) pixel values, which cause ambiguities in image interpretation for physicians and biologists. We propose a multiview Hilbert transformation method to recover the unipolar initial pressure for full-ring PACT. The effectiveness of the proposed algorithm was first validated by numerical simulations and then demonstrated with ex vivo mouse brain structural imaging and in vivo mouse whole-body imaging.

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High‐resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo

Cited by 108 publications

References 28 publications

Dichroism-sensitive photoacoustic computed tomography

Dichroism-sensitive photoacoustic computed tomography

Hand‐held, clinical dual mode ultrasound ‐ photoacoustic imaging of rat urinary bladder and its applications

Multiview Hilbert transformation in full-ring transducer array-based photoacoustic computed tomography

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