High-resolution magic angle spinning 1H NMR spectroscopy of metabolic changes in rabbit lens after treatment with dexamethasone combined with UVB exposure

Abstract

HR-MAS (1)H NMR spectroscopy was found to be an efficient tool for analysis of intact lens tissues. High-resolution NMR spectra of intact lens tissue enabled metabolic changes to be quantified. Long-term treatment with dexamethasone combined with UVB exposure induced substantial metabolic changes, dominated by osmolytic regulation processes and loss of glutathione.

Acute irreversible corticol cataracts in prolonged topical corticosteriod overuse for chronic eczema

“…Sorbitol accumulation, inhibition of glucose-6-phosphate dehydrogenase and formation of disulfide-linked protein aggregates have also been implicated. 5 Patients should be cautioned against overuse of dermal corticosteroids, and warned of potential systemic absorption and possible ocular side effects. …”

The effect of single and repeated UVB radiation on rabbit lens

“…In the high-and middle-field region (0.80-4.75 ppm), 13 different metabolites were identified and subjected for detailed analysis. Assignment of peaks was based on one-and two-dimensional 1 H-NMR techniques in combination with previously reported data [13,15,23,26,27,29,32,33]. Representative HR-MAS 1 H NMR spectra of control and UVB irradiated rabbit lenses related to lens wet weights are shown in Fig.…”

“…The baseline disruptions caused by high level of lenticular proteins were successfully attenuated using the CPMG spin echo pulse sequence [22], and thus the relative quantification method established by Saether [29] could be performed with high precision. The main amount of the overall spectral intensity was formed by resonances from the metabolites found in the middle-and high-field region of the NMR spectra (0.80 -4.75 ppm).…”

“…As an internal shift reference, 0.25 mM of TSP (sodium-3′-trimethylsilyl-propionate-2,2,3,3-d 4 ) was used. To obtain one-and two-dimensional spectra, the spectrometer settings and acquisition parameters were adjusted as in previous studies [29]. Spectra analysis was conducted using special software for analysis of complex mixtures (AMIX, Bruker BioSpin).…”

“…Spectra analysis was conducted using special software for analysis of complex mixtures (AMIX, Bruker BioSpin). Peak areas were measured using absolute intensities and were related to the wet weight of the samples [29].…”