High-Resolution Proton Magnetic Resonance Studies of Gelatin Solutions and Gels

Rose, Philip I.; Gross, Stanley J.

doi:10.1080/00223638.1975.11737811

Cited by 11 publications

(4 citation statements)

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“…58,59 Due to the high demand for decreasing the duration of fracture healing and enhancing the quality of bone healing and fracture fusions, several strategies have been developed including employing electromagnetic stimuli. 58,61 For this purpose, the scaffolds are required to be conductive to locally deliver the electromagnetic stimuli to the site of the injury. Although the electromagnetic stimuli was not used in this paper, it is an important subject for the authors' future studies (both in vitro and in vivo) and it is hypothesized that this technique may make the tissue engineering methods applicable for large bone defects.…”

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confidence: 99%

3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

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confidence: 99%

3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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“…Signal assignment in the NMR spectra of gelatin ( Fig. 3b) corresponds to Rodin & Izmailova (1996); Rose & Gross (1975); Silverstein et al (2005) (Table 1). In the spectra of both biopolymers there is a high-field shift of the signals when the temperature is lowered.…”

Section: Resultsmentioning

confidence: 88%

“…The contribution of different intermolecular interactions to the general mechanism of complex formation was assessed through 1 H NMR spectroscopy. This method of investigation makes it possible not only to identify carrageenans and analyse their structure (De Araujo et al, 2013;Van de Velde, Knutsen, Usov, Rollema, & Cerezo, 2002) but also to make qualitative conclusions about the mobility of certain parts of the κ-carrageenan carrabiose unit (Sebaaly et al, 2012) and several amino-acid residues of gelatin (Izmailova, Derkach, Rodin, Yampol'skaya, & Tulovskaya, 2000;Rodin & Izmailova, 1996;Rose & Gross, 1975). Additional data were collected by UV absorption spectroscopy, light scattering dispersion and capillary viscosity.…”

Section: Introductionmentioning

confidence: 98%

Formation of κ-carrageenan–gelatin polyelectrolyte complexes studied by 1H NMR, UV spectroscopy and kinematic viscosity measurements

Voron’ko

Derkach

Vovk

et al. 2016

Carbohydrate Polymers

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“…Lines in the 1 H NMR spectra of the polysaccharide ( Figure 3 ) are assigned to the protons in the the carrabious block of κ-carrageenan according to the data in [ 95 , 96 , 97 ]. Lines in the 1 H NMR spectra of gelatin are assigned to specific types of protons included in the certain amino acid residue in accordance with [ 35 , 98 , 99 ]. Spectrum analysis shows that the ratio of the integrated intensities of the individual lines of the spectrum is consistent with the content of amino acid residues in the gelatin macromolecule [ 100 , 101 ].…”

Section: The Role Of Electrostatic Interactions Hydrogen Bonds and Hy...mentioning

confidence: 99%

Intermolecular Interactions in the Formation of Polysaccharide-Gelatin Complexes: A Spectroscopic Study

2022

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Gelatin, due to its gelling and stabilizing properties, is one of the widely used biopolymers in biotechnology, medicine, pharmaceuticals, and the food industry. One way to modify the characteristics of gelatin is molecular modification by forming non-covalent polyelectrolyte complexes with polysaccharides based on the self-organization of supramolecular structures. This review summarizes recent advances in the study of various types and the role of intermolecular interactions in the formation of polysaccharide-gelatin complexes, and conformational changes in gelatin, with the main focus on data obtained by spectroscopic methods: UV, FT-IR, and 1H NMR spectroscopy. In the discussion, the main focus is on the complexing polysaccharides of marine origin-sodium alginate, κ-carrageenan, and chitosan. The prospects for creating polysaccharide-gelatin complexes with desired physicochemical properties are outlined.

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High-Resolution Proton Magnetic Resonance Studies of Gelatin Solutions and Gels

Cited by 11 publications

References 0 publications

3D conductive nanocomposite scaffold for bone tissue engineering

3D conductive nanocomposite scaffold for bone tissue engineering

Formation of κ-carrageenan–gelatin polyelectrolyte complexes studied by 1H NMR, UV spectroscopy and kinematic viscosity measurements

Intermolecular Interactions in the Formation of Polysaccharide-Gelatin Complexes: A Spectroscopic Study

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