High resolution RBA-banding comparison between early prometaphase chromosomes of cattle (<i>Bos taurus</i> L.) and goat (<i>Capra hircus</i> L.) at 700 band level

Abstract

High resolution RBA-banded early prometaphase chromosomes of cattle (Bos taurus L.) and goat (Capra hircus L.), from thymidine synchronized lymphocyte cultures, are compared at a level of 700 bands per haploid genome, with the purpose of detecting the extent of banding homologies between the two species and improving the resolution level of the ISCNDA (1989) standardized RBA-banded karyotypes. The results demonstrate that, at this level of resolution, at least 10 autosomes can be fully homologized between the two species, namely chromosomes 11, 16, 17, 18, 20, 21, 22, 23, 24 and 26, whereas the remaining autosomes show variations in some regions which require further investigations on more elongated chromosomes. Such investigations should also involve the use of GTG, GBG and RBG-banding techniques. These variations concern basically the relative distance of the bands from the centromeres or telomeres, appearance of subbands, and clustering of some positive bands.

Localization by FISH of the 31 Texas nomenclature type I markers to both Q- and R-banded bovine chromosomes

“…Because of the close similarity among bovine, caprine and ovine karyotypes (Evans et al, 1973;Schnedl and Czaker, 1974;Hayes et al, 1991;Kaftanovskaya and Serov, 1994;Iannuzzi and Di Meo, 1995;Di Berardino and Burguete, 1998), the data accumulated for cattle can serve as a basis to define the International System for Chromosome Nomenclature of Domestic Bovids. This will contribute essential material for all cytogeneticists interested in gene mapping and comparative mapping, analysing chromosome rearrangements or studying the chromosome phylogeny of bovidae species.…”

International System for Chromosome Nomenclature of Domestic Bovids (ISCNDB 2000)

“…For this reason, sheep ideograms have been modified as compared to those reported by Ansari et al (1999) so that band numbering, band intensities and landmarks are as close as possible to those of bovine ideograms. Since cattle and goat banded chromosomes (Hayes et al, 1991;Iannuzzi and Di Meo, 1995;Di Berardino and Burghete, 1998) are very similar, most cattle G-and R-banded ideograms can be applied to goat chromosomes. However, due to small differences in the band pattern of a few chromosomes, sheep ideograms of chromosomes 8, 9, X and Y should be used for goat chromosomes 9, 14, X and Y.…”

Comparative FISH mapping in river buffalo and sheep chromosomes: assignment of forty autosomal type I loci from sixteen human chromosomes

“…Comparative chromosome banding studies have demonstrated extensive chromosome (arm) homoeology among bovids, especially in the autosomes (Buckland and Evans, 1978;Hayes et al, 1991;Gallagher and Womack, 1992;Kaftanovskaya and Serov, 1994;Iannuzzi and Di Meo, 1995;Di Berardino and Burghete, 1998). These homoeologies have been confirmed by both comparative FISH mapping (Hayes et al, 1993;Iannuzzi et al, 1993Iannuzzi et al, , 1999aIannuzzi et al, , 2000aLopez-Corrales et al, 1998) and linkage mapping studies (Crawford et al, 1995;de Gortari et al, 1998).…”