High-resolution temporally resolved chemiluminescence based on double-layered 3D microfluidic paper-based device for multiplexed analysis

Li, Fang; Liu, Jiachang; Guo, Lei; Wang, Jihang; Zhang, Kaiqi; He, Jianbo; Cui, Hua

doi:10.1016/j.bios.2019.111472

Cited by 62 publications

(41 citation statements)

References 28 publications

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“…A challenge with temporally resolved CL is that it requires a slower flow rate, which lengthens the assay time. Li et al [ 33 ] addressed this issue by creating a μPAD with larger detection regions connected in series by smaller channels, which allowed them to detect CL peaks with a single sensor and maintain a continuous flow rate. They achieved LODs of 0.7–15 μM for small biomolecules such as glucose, but generating H 2 O 2 with biomarker-specific oxidases limits the method to biomolecules with available oxidases.…”

Section: Spectroscopic Detectionmentioning

confidence: 99%

Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices

Mitchell

Esene

2021

Anal Bioanal Chem

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Graphical abstract Microfluidic devices can provide a versatile, cost-effective platform for disease diagnostics and risk assessment by quantifying biomarkers. In particular, simultaneous testing of several biomarkers can be powerful. Here, we critically review work from the previous 4 years up to February 2021 on developing microfluidic devices for multiplexed detection of biomarkers from samples. We focus on two principal approaches: electrical and optical detection methods that can distinguish and quantify biomarkers. Both electrical and spectroscopic multiplexed detection strategies are being employed to reach limits of detection below clinical sample levels. Some of the most promising strategies for point-of-care assays involve inexpensive materials such as paper-based microfluidic devices, or portable and accessible detectors such as smartphones. This review does not comprehensively cover all multiplexed microfluidic biomarker studies, but rather provides a critical evaluation of key work and suggests promising prospects for future advancement in this field. Electrical and optical multiplexing are powerful approaches for microfluidic biomarker analysis.

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Section: Spectroscopic Detectionmentioning

confidence: 99%

Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices

Mitchell

Esene

2021

Anal Bioanal Chem

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“…For detection, this technique can reduce background noise without the need for an external light source, but a portable chemiluminescence reader must be operated in the dark, which makes device fabrication more complex. This detection method was used with μPADs for biomarker detection [ 145 ], immunoassay [ 146 ], biomolecule detection [ 147 ], environmental monitoring [ 148 ], and food safety control [ 149 ]. In recent years, chemiluminescence sensing has become popular for clinical biomarker detection and immunoassays due to its wide dynamic range and complete automation.…”

Section: Detection Techniquesmentioning

confidence: 99%

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

et al. 2020

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Microfluidic paper-based analytical devices (µPADs) have become promising tools offering various analytical applications for chemical and biological assays at the point-of-care (POC). Compared to traditional microfluidic devices, µPADs offer notable advantages; they are cost-effective, easily fabricated, disposable, and portable. Because of our better understanding and advanced engineering of µPADs, multistep assays, high detection sensitivity, and rapid result readout have become possible, and recently developed µPADs have gained extensive interest in parallel analyses to detect biomarkers of interest. In this review, we focus on recent developments in order to achieve µPADs with high-throughput capability. We discuss existing fabrication techniques and designs, and we introduce and discuss current detection methods and their applications to multiplexed detection assays in relation to clinical diagnosis, drug analysis and screening, environmental monitoring, and food and beverage quality control. A summary with future perspectives for µPADs is also presented.

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“…Plenty of reports are available on colorimetric biosensors integrated with microfluidics, e.g., [27,28,[96][97][98]; most of the reports highlighted the features of cost-effectiveness and miniaturization. Different from three other kinds of biosensors, the materials adopted for the integrated microfluidic structures are usually not PDMS, but paper for the majority and cloth in some researches.…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

Application of Microfluidics in Biosensors

Wang¹,

Ren²,

Zhang³

2020

Advances in Microfluidic Technologies for Energy and Environmental Applications

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High-resolution temporally resolved chemiluminescence based on double-layered 3D microfluidic paper-based device for multiplexed analysis

Cited by 62 publications

References 28 publications

Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices

Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

Application of Microfluidics in Biosensors

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