High resolution voltammetric and field-effect transistor readout of carbon fiber microelectrode biosensors

Cho, Whirang; Rafi, Harmain; Cho, Seulki; Balijepalli, Arvind; Zestos, Alexander G.

doi:10.1039/d2sd00023g

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…Measurements of cortisol in urine are observed in prior studies and are a standard diagnostic for cortisol detection [ 42 ] and that of other molecules [ 43 ]. Urine was a convenient sample to measure as a biological sample because of its ease of accessibility and due to the ability to alter pH whose fluctuations can be measured at the electrode surface [ 44 ]. Urine contains uric acid, urea, and other components that make it slightly more acidic than buffer at a physiological pH.…”

Section: Resultsmentioning

confidence: 99%

Carbon Electrode Sensor for the Measurement of Cortisol with Fast-Scan Cyclic Voltammetry

Hadad,

Zestos

2023

Biosensors

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Cortisol is a vital steroid hormone that has been known as the “stress hormone”, which is elevated during times of high stress and anxiety and has a significant impact on neurochemistry and brain health. The improved detection of cortisol is critically important as it will help further our understanding of stress during several physiological states. Several methods exist to detect cortisol; however, they suffer from low biocompatibility and spatiotemporal resolution, and they are relatively slow. In this study, we developed an assay to measure cortisol with carbon fiber microelectrodes (CFMEs) and fast-scan cyclic voltammetry (FSCV). FSCV is typically utilized to measure small molecule neurotransmitters by producing a readout cyclic voltammogram (CV) for the specific detection of biomolecules on a fast, subsecond timescale with biocompatible CFMEs. It has seen enhanced utility in measuring peptides and other larger compounds. We developed a waveform that scanned from −0.5 to −1.2 V at 400 V/s to electro-reduce cortisol at the surface of CFMEs. The sensitivity of cortisol was found to be 0.87 ± 0.055 nA/μM (n = 5) and was found to be adsorption controlled on the surface of CFMEs and stable over several hours. Cortisol was co-detected with several other biomolecules such as dopamine, and the waveform was fouling resistant to repeated injections of cortisol on the surface of the CFMEs. Furthermore, we also measured exogenously applied cortisol into simulated urine to demonstrate biocompatibility and potential use in vivo. The specific and biocompatible detection of cortisol with high spatiotemporal resolution will help further elucidate its biological significance and further understand its physiological importance and impact on brain health.

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Section: Resultsmentioning

confidence: 99%

Carbon Electrode Sensor for the Measurement of Cortisol with Fast-Scan Cyclic Voltammetry

Hadad,

Zestos

2023

Biosensors

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“…6 -with drug usage [125] Carbon fiber (CF) FSCV Ex-vivo/rat brain slices -na-3 to 6 [126] Abbreviations: W-ME: Tungsten microelectrode, ISP/IrO 2 : integrated solid-state: CF-H + /ISE carbon fiber-based proton-selective electrodes. matic reactions progressed to produce ammonium proton consumption or hydroxide generation of ions (NH 4…”

Section: R E V I E W T H E C H E M I C a L R E C O R Dmentioning

confidence: 99%

Advances in pH Sensing: From Traditional Approaches to Next‐Generation Sensors in Biological Contexts

Hassan Akhtar,

Azhar Hayat Nawaz,

Abbas

et al. 2024

The Chemical Record

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AbstractpH has been considered one of the paramount factors in bodily functions because most cellular tasks exclusively rely on precise pH values. In this context, the current techniques for pH sensing provide us with the futuristic insight to further design therapeutic and diagnostic tools. Thus, pH‐sensing (electrochemically and optically) is rapidly evolving toward exciting new applications and expanding researchers’ interests in many chemical contexts, especially in biomedical applications. The adaptation of cutting‐edge technology is subsequently producing the modest form of these biosensors as wearable devices, which are providing us the opportunity to target the real‐time collection of vital parameters, including pH for improved healthcare systems. The motif of this review is to provide insight into trending tech‐based systems employed in real‐time or in‐vivo pH‐responsive monitoring. Herein, we briefly go through the pH regulation in the human body to help the beginners and scientific community with quick background knowledge, recent advances in the field, and pH detection in real‐time biological applications. In the end, we summarize our review by providing an outlook; challenges that need to be addressed, and prospective integration of various pH in vivo platforms with modern electronics that can open new avenues of cutting‐edge techniques for disease diagnostics and prevention.

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“…Electrochemical techniques have several advantages, including a fast response, high selectivity and sensitivity (comes from the electrochemical reaction depends on the applied potential, which can be regulated as required), relatively low preparation costs, simple operational requirements, and easy miniaturization. [17][18][19][20][21][22][23] These techniques can be used to overcome the aforementioned obstacles for facile on-site environmental analysis. They can also be used for high-fidelity antibiotic quantification.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical quantification of sulfamethoxazole antibiotic in environmental water using zeolitic imidazolate framework (ZIF)-derived single-atom cobalt catalyst in nitrogen-doped carbon nanostructures

Wang

Ding

et al. 2022

Sens. Diagn.

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High resolution voltammetric and field-effect transistor readout of carbon fiber microelectrode biosensors

Abstract: Rapid and sensitive pH measurements with increased spatiotemporal resolution are imperative to probe neurochemical signals and illuminate brain function. We interfaced carbon fiber microelectrode (CFME) sensors with both fast scan...

Cited by 3 publications

References 48 publications

Carbon Electrode Sensor for the Measurement of Cortisol with Fast-Scan Cyclic Voltammetry

Carbon Electrode Sensor for the Measurement of Cortisol with Fast-Scan Cyclic Voltammetry

Advances in pH Sensing: From Traditional Approaches to Next‐Generation Sensors in Biological Contexts

Electrochemical quantification of sulfamethoxazole antibiotic in environmental water using zeolitic imidazolate framework (ZIF)-derived single-atom cobalt catalyst in nitrogen-doped carbon nanostructures

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