High-resolution x-ray absorption spectroscopy studies of metal compounds in neurodegenerative brain tissue

Collingwood, Joanna F.; Mikhaylova, Albina; Davidson, Mark R.; Batich, Christopher D.; Streit, Wolfgang J.; Eskin, Thomas A.; Terry, Jeff; Barrea, Raúl A.; Underhill, Royale S.; Dobson, Jon

doi:10.1088/1742-6596/17/1/009

Cited by 20 publications

(20 citation statements)

References 22 publications

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“…Sample volume was about 1 cm 3 . Liver samples were obtained from two other female donors (donors 3 and 4) and two male donors (donors 5 and 6) aged 75-96 and spleen samples were removed from donors 5 and 6, with a volume ranging from 1 to 8 cm 3 . The tissue samples showed no signs of liver or spleen pathology.…”

Section: Samplesmentioning

confidence: 99%

Variable‐field relaxometry of iron‐containing human tissues: a preliminary study

Hocq

Brouette

Saussez

et al. 2009

Contrast Media & Molecular

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Excess iron is found in brain nuclei from neurodegenerative patients (with Parkinson's, Alzheimer's and Huntington's diseases) and also in the liver and spleen of cirrhosis, hemochromatosis and thalassaemia patients. Ferritin, the iron-storing protein of mammals, is known to darken T(2)-weighted MR images. Understanding NMR tissue behavior may make it possible to detect those diseases, to follow their evolution and finally to establish a protocol for non-invasive measurement of an organ's iron content using MRI methods. In this preliminary work, the MR relaxation properties of embalmed iron-containing tissues were studied as well as their potential correlation with the iron content of these tissues. Relaxometric measurements (T(1) and T(2)) of embalmed samples of brain nuclei (caudate nucleus, dentate nucleus, globus pallidus, putamen, red nucleus and substantia nigra), liver and spleen from six donors were made at different magnetic fields (0.00023-14 T). The influence of the inter-echo time on transverse relaxation was also studied. Moreover, iron content of tissues was determined by inductively coupled plasma atomic emission spectroscopy. In brain nuclei, 1/T(2) increases quadratically with the field and depends on the inter-echo time in CPMG sequences at high fields, both features compatible with an outer sphere relaxation theory. In liver and spleen, 1/T(2) increases linearly with the field and depends on the inter-echo time at all fields. In our study, a correlation between 1/T(2) and iron concentration is observed. Explaining the relaxation mechanism for these tissues is likely to require a combination of several models. The value of 1/T(2) at high field could be used to evaluate iron accumulation in vivo. In the future, confirmation of those features is expected to be achieved from measurements of fresh (not embalmed) human tissues.

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Section: Samplesmentioning

confidence: 99%

Variable‐field relaxometry of iron‐containing human tissues: a preliminary study

Hocq

Brouette

Saussez

et al. 2009

Contrast Media & Molecular

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“…According to Jackson et al (2006), the complexity of sample preparation and instrumental techniques, the expense of instrument, and the number of analyte ions that can be determined are the important criteria for selecting a surface analytical technique. Based on literatures reported previously, besides the abovementioned fluorescence microscopic techniques, proton-induced X-ray emission (PIXE) (Thong et al, 1999;Robertson et al, 2002;Augustyniak et al, 2006;Reinert et al, 2007), microprobe synchrotron X-ray fluorescence (ASXRF) (Ide-Ektessabi, Kawakami, & Watt, 2004;Flinn et al, 2005;Serpa et al, 2008), synchrotron X-ray absorption spectroscopy (SXAS) (Collingwood et al, 2005;Mikhaylova et al, 2005), secondary ion mass spectrometry (SIMS) (Burns, 1981;Clerc, Fourre, & Fragu, 1997;Aranyosiova et al, 2008), and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) (Feldmann, Kindness, & Ek, 2002;Becker et al, 2005aBecker et al, ,b, 2007aBecker et al, ,b, 2008aJackson et al, 2006;Dobrowolska et al, 2008;Sela et al, 2007;Zoriy et al, 2008) are the most widely applied surface analytical techniques for elemental imaging in brain tissues.…”

Section: Surface Analytical Techniquesmentioning

confidence: 99%

In vivomonitoring of the transfer kinetics of trace elements in animal brains with hyphenated inductively coupled plasma mass spectrometry techniques

Su¹,

Sun²,

Tzeng³

et al. 2009

Mass Spectrom. Rev.

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The roles of metal ions to sustain normal function and to cause dysfunction of neurological systems have been confirmed by various studies. However, because of the lack of adequate analytical method to monitor the transfer kinetics of metal ions in the brain of a living animal, research on the physiopathological roles of metal ions in the CNS remains in its early stages and more analytical efforts are still needed. To explicitly model the possible links between metal ions and physiopathological alterations, it is essential to develop in vivo monitoring techniques that can bridge the gap between metalloneurochemistry and neurophysiopathology. Although inductively coupled plasma mass spectrometry (ICP-MS) is a very powerful technique for multiple trace element analyses, when dealing with chemically complex microdialysis samples, the detection capability is largely limited by instrumental sensitivity, selectivity, and contamination that arise from the experimental procedure. As a result, in recent years several high efficient and clean on-line sample pretreatment systems have been developed and combined with microdialysis and ICP-MS for the continuous and in vivo determination of the concentration-time profiles of metal ions in the extracellular space of rat brain. This article reviews the research relevant to the development of analytical techniques for the in vivo determination of dynamic variation in the concentration levels of metal ions in a living animal.

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“…Later that same year, Collingwood et al [62,63] demonstrated the application of this technique to AD tissue sections. This work provided the first map of iron distribution in AD tissue in which specific iron anomalies (areas of high iron concentration) were not only located but characterized with 5 lm spatial resolution.…”

Section: Iron-containing Compounds Related To Alzheimer Diseasementioning

confidence: 99%

Iron: The Redox-active Center of Oxidative Stress in Alzheimer Disease

et al. 2007

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Although iron is essential in maintaining the function of the central nervous system, it is a potent source of reactive oxygen species. Excessive iron accumulation occurs in many neurodegenerative diseases including Alzheimer disease (AD), Parkinson's disease, and Creutzfeldt-Jakob disease, raising the possibility that oxidative stress is intimately involved in the neurodegenerative process. AD in particular is associated with accumulation of numerous markers of oxidative stress; moreover, oxidative stress has been shown to precede hallmark neuropathological lesions early in the disease process, and such lesions, once present, further accumulate iron, among other markers of oxidative stress. In this review, we discuss the role of iron in the progression of AD.

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High-resolution x-ray absorption spectroscopy studies of metal compounds in neurodegenerative brain tissue

Cited by 20 publications

References 22 publications

Variable‐field relaxometry of iron‐containing human tissues: a preliminary study

Variable‐field relaxometry of iron‐containing human tissues: a preliminary study

In vivomonitoring of the transfer kinetics of trace elements in animal brains with hyphenated inductively coupled plasma mass spectrometry techniques

Iron: The Redox-active Center of Oxidative Stress in Alzheimer Disease

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