High Salt Diet Affects Renal Sodium Excretion and ERRα Expression

Abstract

Kidneys regulate the balance of water and sodium and therefore are related to blood pressure. It is unclear whether estrogen-related receptor α (ERRα), an orphan nuclear receptor and transcription factor highly expressed in kidneys, affects the reabsorption of water and sodium. The aim of this study was to determine whether changes in the expressions of ERRα, Na+/K+-ATPase and epithelial sodium channel (ENaC) proteins affected the reabsorption of water and sodium in kidneys of Dahl salt-sensitive (DS) rats. SS.13BN rats, 98% homologous to the DS rats, were used as a normotensive control group. The 24 h urinary sodium excretion of the DS and SS.13BN rats increased after the 6-week high salt diet intervention, while sodium excretion was increased in DS rats with daidzein (agonist of ERRα) treatment. ERRα expression was decreased, while β- and γ-ENaC mRNA expressions were increased upon high sodium diet treatment in the DS rats. In the chromatin immunoprecipitation (CHIP) assay, positive PCR signals were obtained in samples treated with anti-ERRα antibody. The transcriptional activity of ERRα was decreased upon high salt diet intervention. ERRα reduced the expressions of β- and γ-ENaC by binding to the ENaC promoter, thereby increased Na+ reabsorption. Therefore, ERRα might be one of the factors causing salt-sensitive hypertension.

The association of urinary uric acid excretion with ambulatory blood pressure values in patients with chronic kidney disease

“…Further, dysfunctional NKA caused by defected membrane of renal tubular cells result in diminished Na + reabsorption and urinary concentration ability [14]. In other words, incremental urinary sodium excretion leads to hypertension [15]. All above is expected to prove that the extent of urinary uric acid excretion, whether it takes the form of FEua or Cur, may be related with the value of blood pressure.…”

Mitochondrial tRNA Mutation and Regulation of the Adiponectin Pathway in Maternally Inherited Hypertension in Chinese Han

“…ERRα, an orphan nuclear receptor, is an important regulator of blood pressure, mainly by affecting the balance of sodium and potassium in the kidneys (Wang et al, 2016). Loss of ERRα expression, whether stimulated by high sodium, low potassium, or high renin, leads to hypotension instead of hypertension.…”

Measurement of cations, anions, and acetate in serum, urine, cerebrospinal fluid, and tissue by ion chromatography

“…Urine electrolyte concentrations typically have a large reported range which is dependent on several factors, but the most prominent is water/food and salt intake (Foss et al 2013;Gomes et al 2017;Osborn et al 2014;Wang et al 2016;Yu et al 2016). As such, most research groups measure 24 h sodium and water balance to help determine water and sodium intake, urine volume and urine sodium excretion (Foss et al 2013;Gomes et al 2017;Osborn et al 2014;Wang et al 2016;Yu et al 2016). Non-24 h urine analysis though can provide insight into progression of disease, including electrolyte imbalance and acid/base disorders (Gray et al 2013;Hamm et al 2015;Waldrop 2008).…”