High-sensitivity fluorescence detection for lung cancer CYFRA21-1 DNA based on accumulative hybridization of quantum dots

Yang, Ling; Ma, Ping’an; Chen, Xiaorui; Cheng, Ziyong; Lin, Jun

doi:10.1039/d1tb02557k

Cited by 14 publications

(6 citation statements)

References 38 publications

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“…Consequently, the SCC-MAG method proves to be a robust, replicable, and highly sensitive approach for analyzing mutation status in liquid biopsies, facilitating the diagnosis of multiple targets through the reuse of multiple probes. Ling Yang and colleagues [ 120 ] synthesized hydrophilic AgInS2@ZnS core-shell quantum dots and magnetic Fe 3 O 4 nanoparticles ( Figure 6 C). They employed a hybridization chain reaction triggered by ctDNA to detect lung-cancer-related CYFRA21-1 DNA [ 121 ].…”

Section: Applications Of Fluorescent Microspheres In Multiplex Nuclei...mentioning

confidence: 99%

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Chen,

Luo,

Ren

et al. 2024

Biosensors

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Traditional single nucleic acid assays can only detect one target while multiple nucleic acid assays can detect multiple targets simultaneously, providing comprehensive and accurate information. Fluorescent microspheres in multiplexed nucleic acid detection offer high sensitivity, specificity, multiplexing, flexibility, and scalability advantages, enabling precise, real-time results and supporting clinical diagnosis and research. However, multiplexed assays face challenges like complexity, costs, and sample handling issues. The review explores the recent advancements and applications of fluorescent microspheres in multiple nucleic acid detection. It discusses the versatility of fluorescent microspheres in various fields, such as disease diagnosis, drug screening, and personalized medicine. The review highlights the possibility of adjusting the performance of fluorescent microspheres by modifying concentrations and carrier forms, allowing for tailored applications. It emphasizes the potential of fluorescent microsphere technology in revolutionizing nucleic acid detection and advancing health, disease treatment, and medical research.

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Section: Applications Of Fluorescent Microspheres In Multiplex Nuclei...mentioning

confidence: 99%

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Chen,

Luo,

Ren

et al. 2024

Biosensors

View full text Add to dashboard Cite

show abstract

“…AgInS 2 QDs, which have been involved in significant research progress in recent years, have not only the excellent properties of QDs but also the advantages of low toxicity and environmental friendliness. Yang et al designed a sensor for the accumulation of AgInS 2 /ZnS QDs via ctDNA-triggered HCR, which triggers a significant change in the fluorescence signal 97 . As shown in Fig.…”

Section: Fluorescence Sensingmentioning

confidence: 99%

Section: Fluorescence Sensingmentioning

confidence: 99%

Current research status of tumor cell biomarker detection

Jiang,

Lin,

Chen

et al. 2023

Microsyst Nanoeng

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With the annual increases in the morbidity and mortality rates of tumors, the use of biomarkers for early diagnosis and real-time monitoring of tumor cells is of great importance. Biomarkers used for tumor cell detection in body fluids include circulating tumor cells, nucleic acids, protein markers, and extracellular vesicles. Among them, circulating tumor cells, circulating tumor DNA, and exosomes have high potential for the prediction, diagnosis, and prognosis of tumor diseases due to the large amount of valuable information on tumor characteristics and evolution; in addition, in situ monitoring of telomerase and miRNA in living cells has been the topic of extensive research to understand tumor development in real time. Various techniques, such as enzyme-linked immunosorbent assays, immunoblotting, and mass spectrometry, have been widely used for the detection of these markers. Among them, the detection of tumor cell markers in body fluids based on electrochemical biosensors and fluorescence signal analysis is highly preferred because of its high sensitivity, rapid detection and portable operation. Herein, we summarize recent research progress in the detection of tumor cell biomarkers in body fluids using electrochemical and fluorescence biosensors, outline the current research status of in situ fluorescence monitoring and the analysis of tumor markers in living cells, and discuss the technical challenges for their practical clinical application to provide a reference for the development of new tumor marker detection methods.

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“…Abnormal concentrations of enzymes, antibodies, , and other proteins secreted by the body are often known to predict the development of certain inflammatory, tumor, or infectious diseases in modern medicine, such as Alzheimer’s disease, asthma, lung cancer, and COVID-19. Numerous methods have been reported for the detection of these markers, such as the fluorescence method, , ELISA (enzyme-linked immunosorbent assay), electrochemiluminescence, and electrochemical detection methods. , However, there are many impediments to the use of these methods for clinical diagnosis, such as being time-consuming and costly . In practice, the difference in marker concentrations between healthy and diseased states can be very small, which poses a great challenge to the accuracy of these methods in clinical diagnosis. − …”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Protein Concentration Monitoring Using Natural Wood Evaporation for Power Generation

Sheng,

Li,

Jia

et al. 2024

Anal. Chem.

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A high-sensitivity, low-cost, self-powered biomass electrochemical biosensor based on the "evaporating potential" theory is developed for protein detection. The feasibility of experimental evaluation methods was verified with a probe protein of bovine serum albumin. The sensor was then used to detect lung cancer marker CYFRA21-1, and the potential of our sensor for clinical diagnosis was demonstrated by serum analysis. This work innovatively exploits the osmotic power generation capability of natural wood to construct a promising electrochemical biosensor that was driven by kinetics during testing. The detection methods used for this sensor, chronoamperometry and AC impedance, showed potential for quantitative analysis and specific detection, respectively. Furthermore, the sensor could facilitate new insights into the development of high-sensitivity, low-cost, and easy-to-use electrochemical biosensors.

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High-sensitivity fluorescence detection for lung cancer CYFRA21-1 DNA based on accumulative hybridization of quantum dots

Abstract: Sensitive detection of circulating tumor DNA (ctDNA) in vitro has attracted growing attention owing to its potential application in diagnostics of cancer. In this study, we synthesized hydrophilic AgInS2@ZnS core-shell...

Cited by 14 publications

References 38 publications

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Recent Advances in and Application of Fluorescent Microspheres for Multiple Nucleic Acid Detection

Current research status of tumor cell biomarker detection

Electrochemical Biosensor for Protein Concentration Monitoring Using Natural Wood Evaporation for Power Generation

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