High-sensitivity lateral flow immunoassay with a fluorescent lanthanide nanoparticle label

Salminen, Teppo; Juntunen, Etvi; Talha, Sheikh M.; Pettersson, Kim

doi:10.1016/j.jim.2018.12.001

Cited by 31 publications

(15 citation statements)

References 48 publications

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“…For this reason, there is a growing need to develop quantitative and reader instrument-based LFIAs. Generally, fluorescent reporters provide superior sensitivity to colloidal gold and allow quantification of analytes 24 , 25 . Among fluorescent reporters, upconverting nanoparticles (UCNPs; Upcon ® nanoparticles, Kaivogen Oy, Finland) are crystalline lanthanide-doped nanometersized particles.…”

Section: Introductionmentioning

confidence: 99%

Glycovariant-based lateral flow immunoassay to detect ovarian cancer–associated serum CA125

et al. 2020

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Cancer antigen 125 (CA125) is a widely used biomarker in monitoring of epithelial ovarian cancer (EOC). Due to insufficient cancer specificity of CA125, its diagnostic use is severely compromised. Abnormal glycosylation of CA125 is a unique feature of ovarian cancer cells and could improve differential diagnosis of the disease. Here we describe the development of a quantitative lateral flow immunoassay (LFIA) of aberrantly glycosylated CA125 which is widely superior to the conventional CA125 immunoassay (CA125IA). With a 30 min read-out time, the LFIA showed 72% sensitivity, at 98% specificity using diagnostically challenging samples with marginally elevated CA125 (35–200 U/mL), in comparison to 16% sensitivity with the CA125IA. We envision the clinical use of the developed LFIA to be based on the substantially enhanced disease specificity against the many benign conditions confounding the diagnostic evaluation and against other cancers.

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Section: Introductionmentioning

confidence: 99%

Glycovariant-based lateral flow immunoassay to detect ovarian cancer–associated serum CA125

et al. 2020

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“…Unfortunately, none of them could match the reliability of lab-based thorough antibody tests [ 147 ]. Some recent reports suggest that nanoparticle-based lateral immunoassays would be considerably more accurate [ 148 ]. Nevertheless, there needs to be further rigorous research on the strip-based tests for at least one or a few of them to be authorized for unrestricted universal usage.…”

Section: Challenges and Pitfallsmentioning

confidence: 99%

Rapid Antibody-Based COVID-19 Mass Surveillance: Relevance, Challenges, and Prospects in a Pandemic and Post-Pandemic World

Augustine

Das

Hasan

et al. 2020

JCM

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The aggressive outbreak of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) as COVID-19 (coronavirus disease-2019) pandemic demands rapid and simplified testing tools for its effective management. Increased mass testing and surveillance are crucial for controlling the disease spread, obtaining better pandemic statistics, and developing realistic epidemiological models. Despite the advantages of nucleic acid- and antigen-based tests such as accuracy, specificity, and non-invasive approaches of sample collection, they can only detect active infections. Antibodies (immunoglobulins) are produced by the host immune system within a few days after infection and persist in the blood for at least several weeks after infection resolution. Antibody-based tests have provided a substitute and effective method of ultra-rapid detection for multiple contagious disease outbreaks in the past, including viral diseases such as SARS (severe acute respiratory syndrome) and MERS (Middle East respiratory syndrome). Thus, although not highly suitable for early diagnosis, antibody-based methods can be utilized to detect past infections hidden in the population, including asymptomatic ones. In an active community spread scenario of a disease that can provide a bigger window for mass detections and a practical approach for continuous surveillance. These factors encouraged researchers to investigate means of improving antibody-based rapid tests and employ them as reliable, reproducible, sensitive, specific, and economic tools for COVID-19 mass testing and surveillance. The development and integration of such immunoglobulin-based tests can transform the pandemic diagnosis by moving the same out of the clinics and laboratories into community testing sites and homes. This review discusses the principle, technology, and strategies being used in antibody-based testing at present. It also underlines the immense prospect of immunoglobulin-based testing and the efficacy of repeated planned deployment in pandemic management and post-pandemic sustainable screenings globally.

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“…Three‐order lower sensitivity has been recorded in comparison to AuNPs‐based LFA. Salminen, Juntunen, Talha, and Pettersson (2019) have used fluorescent europium(III) chelate‐doped NP reporter label and a higher sensitivity (100‐fold lower than AuNPs) of LFA detection has been achieved. The coating of AuNPs, silver enhancement, and gold enlargement have also been proposed for improving the sensitivity of LFA devices (Lin, Uzunoglu, & Stanciu, 2018; Linares, Kubota, Michaelis, & Thalhammer, 2012; Rodríguez, Covián, García, & Blanco‐López, 2016; Rohrman, Leautaud, Molyneux, & Richards‐Kortum, 2012).…”

Section: Poc Testing Devicesmentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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The authentication of animal species is an important issue due to an increasing trend of adulteration and mislabeling of animal species in processed meat products. Polymerase chain reaction is the most sensitive and specific technique for nucleic acid‐based animal species detection. However, it is a time‐consuming technique that requires costly thermocyclers and sophisticated labs. In recent times, there is a need of on‐site detection by point‐of‐care (POC) testing methods and devices under low‐resource settings. These POC devices must be affordable, sensitive, specific, user‐friendly, rapid and robust, equipment free, and delivered to the end users. POC devices should also confirm the concept of micro total analysis system. This review discusses POC testing methods and devices that have been developed for meat species identification. Recent developments in lateral flow assay‐based devices for the identification of animal species in meat products are also reviewed. Advancements in increasing the efficiency of lateral flow detection are also discussed.

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High-sensitivity lateral flow immunoassay with a fluorescent lanthanide nanoparticle label

Cited by 31 publications

References 48 publications

Glycovariant-based lateral flow immunoassay to detect ovarian cancer–associated serum CA125

Glycovariant-based lateral flow immunoassay to detect ovarian cancer–associated serum CA125

Rapid Antibody-Based COVID-19 Mass Surveillance: Relevance, Challenges, and Prospects in a Pandemic and Post-Pandemic World

Rapid point‐of‐care testing methods/devices for meat species identification: A review

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