High-sensitivity qualitative and quantitative analysis of human, bovine and goat milk glycosphingolipids using HILIC-MS/MS with internal standards

Li, Zhenhua; Wang, Xiaoqin; Deng, Xiaoli; Song, Jiansen; Yang, Yongzhen; Liao, Yujie; Gong, Guiping; Huang, Linjuan; Lu, Yu; Wang, Zhongfu

doi:10.1016/j.carbpol.2023.120795

Cited by 5 publications

(17 citation statements)

References 69 publications

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“…Due to the intrinsic anionic characteristics of milk GSL, online HILIC-MS/MS was operated in negative-ion mode. The glycosyl and ceramide composition of each GSL was determined according to the MS and MS/MS data, typical biosynthesis of human milk GSLs, , GlycoWorkbench software calculations, and previous studies. ,− The GSL species, mass-to-charge ratios ( m / z ), presumptive structure of the oligosaccharide moiety, ceramide composition, and other characteristics are listed in Table S1. A total of 101 different GSLs were detected, including 16 GB and 85 gangliosides with 1–3 sialic acid modifications in the glycan portion.…”

Section: Resultsmentioning

confidence: 99%

“…Currently, infant formula is primarily based on bovine milk, with others based on caprine milk and other milk sources. However, the composition and distribution of GSLs in bovine and caprine milk differ from those in human milk, with mature human milk being dominated by GM1, while goat and bovine milk are dominated by GD3 . Although many foods contain gangliosides, the only food source for infants aged 0–6 months is mothers’ milk or formula powder.…”

Section: Resultsmentioning

confidence: 99%

“…First, 3 mL of pooled Se+ milk and 3 mL of pooled Se– milk were individually mixed with 6 mL of anhydrous methanol in a 10 mL tube and incubated at 37 °C for 2 h. Next, the mixture was centrifuged at 3500 rpm for 40 min, after which the upper fraction containing GSLs was carefully collected and dried by vacuum suction. The analytes were dissolved in 1 mL of water, and GSLs were enriched using a Sep-Pak C18 column . The column was preactivated with a graded aqueous methanol solution and equilibrated with 12 mL of pure water.…”

Section: Methodsmentioning

confidence: 99%

“…Online HILIC-MS/MS analysis of human milk GSLs was conducted using electrospray ionization linear ion trap (ESI)-MS combined with an HPLC system (LTQ-XL; Thermo Fisher Scientific) equipped with a HILIC column (SHIMSEM Ankylo Amide, 4.6 mm × 250 mm, 5 μm; SHIMADZU, Kyoto, Japan) set to 30 °C. The flow rate was 800 μL/min, and the separation conditions were as described previously . For MS and MS/MS analyses, negative-mode MS was adopted with an electrospray voltage of 4 kV, a sheath gas flow rate of 38.0 arb, a capillary heating temperature of 370 °C, and a scan range of 200–2000 m / z .…”

Section: Methodsmentioning

confidence: 99%

“…For the quantitative analysis of human milk GSLs, the commercial GM1 standard was selectively oxidized and derivatized with AEAB . The resulting GM1-AEAB derivative showed similar polarity but different molecular masses compared to milk GSLs.…”

Section: Methodsmentioning

confidence: 99%

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Comparative Analysis of Human Milk Glycosphingolipids from Different Secretor Mothers Using HILIC-MS/MS

Wang,

Liu,

Rong

et al. 2023

J. Agric. Food Chem.

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Glycosphingolipids participate in brain development, intestinal tract maturation, and defense against gut pathogens. Here, we performed a qualitative and quantitative comparison of milk glycosphingolipids from secretors and nonsecretors. Hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry was employed, along with an internal standard, to resolve the complications presented by the fact that glycosphingolipids are structurally diverse, varying in glycan composition and ceramide. In total, 101 glycosphingolipids were detected, of which 76 were reported for the first time, including fucose-modified neutral glycosphingolipids. Seventy-eight glycosphingolipids differed significantly between secretor and nonsecretor milk (p < 0.05), resulting in higher levels of certain neutral species (p < 0.001) but lower levels of fucose-modified monosialylated and disialylated species in secretor mothers (p < 0.01). In both milk types, the most abundant glycosphingolipids were of the monosialylated type, followed by disialylated, neutral, and trisialylated ones. Notably, fucose-modified monosialylated glycosphingolipids accounted for the highest proportion.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Comparative Analysis of Human Milk Glycosphingolipids from Different Secretor Mothers Using HILIC-MS/MS

Wang,

Liu,

Rong

et al. 2023

J. Agric. Food Chem.

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Exploring the health benefits and functional properties of goat milk proteins

ALKaisy

Al-Saadi

Al-Rikabi

et al. 2023

Food Science & Nutrition

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Goat milk proteins are unique in their nutritional and functional properties and have become increasingly popular in recent years. A variety of methods have been studied for extracting and isolating these proteins, with coprecipitation being a particularly effective approach. Compared to cow milk proteins, goat milk proteins contain higher levels of certain amino acids such as tryptophan and cysteine, while maintaining similar nutritional properties. Additionally, they have superior functional properties, including better emulsifying and foaming properties, which make them an attractive option for developing new food products. Research has shown that goat milk proteins have several health benefits, including immunomodulatory effects, allergy management, anti‐inflammatory, and antioxidant effects, as well as antimicrobial and anticancer properties. They have the potential to be used as a treatment for autoimmune diseases, allergies, and other immune system disorders due to their ability to modulate the production of cytokines and other immune system components. Furthermore, their antimicrobial properties can help prevent the growth of harmful bacteria and reduce the risk of infection. Future research will focus on the potential of goat milk proteins as a functional food ingredient, their effects on gut health and microbiota, and their therapeutic potential for various health conditions. This research may lead to the development of new functional foods that promote health and prevent disease, and potentially pave the way for the use of goat milk proteins as a therapeutic agent for various health conditions.

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High sensitivity profiling of N-glycans from mouse serum using fluorescent imidazolium tags by HILIC electrospray ionisation spectrometry

Zhang,

et al. 2024

Carbohydrate Polymers

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High-sensitivity qualitative and quantitative analysis of human, bovine and goat milk glycosphingolipids using HILIC-MS/MS with internal standards

Cited by 5 publications

References 69 publications

Comparative Analysis of Human Milk Glycosphingolipids from Different Secretor Mothers Using HILIC-MS/MS

Comparative Analysis of Human Milk Glycosphingolipids from Different Secretor Mothers Using HILIC-MS/MS

Exploring the health benefits and functional properties of goat milk proteins

High sensitivity profiling of N-glycans from mouse serum using fluorescent imidazolium tags by HILIC electrospray ionisation spectrometry

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