High specific activity tritiation of the pyridazin-3-one histamine H3 receptor inverse agonist CEP-27088

Tritium NMR: A compilation of data and a practical guide

“…This was necessary to assist in vitro and in vivo biological studies. In this line, Andrews et al [ 64 ] performed a high specific activity tritiation of the pyridazin‐3‐one histamine H 3 receptor inverse agonist CEP‐27088. The proton decoupled tritium NMR spectrum of this compound contained sharp signals centered at 7.08 ppm providing the evidence that tritium had been regiospecifically inserted at the two o ‐positions, as shown in Figure 42.…”

“…Tritium proton decoupled NMR spectrum of tritiated pyridazin‐3‐one histamine H 3 receptor inverse agonist CEP‐27088 recorded in DMSO‐ d 6 at 320 MHz. Reproduced with minor editing privilege from Andrews et al [ 64 ] with the permission of Elsevier…”

“…In order to better understand the biology of the nightshade alkaloids, the authors performed tritium labeling and 3 H NMR monitoring of several selected F I G U R E 4 2 Tritium proton decoupled NMR spectrum of tritiated pyridazin-3-one histamine H 3 receptor inverse agonist CEP-27088 recorded in DMSO-d 6 at 320 MHz. Reproduced with minor editing privilege from Andrews et al [64] with the permission of Elsevier F I G U R E 4 3 Tritium proton decoupled NMR spectrum of the agonist (+)-SKF-10,047 recorded in CD 3 OD at 320 MHz. Reproduced with minor editing privilege from Ahern et al [65] with the permission of Springer Nature members of this family including [ 3 H] dihydrocapsaicin and [N-methyl-3 H] atropine, which are shown accordingly, on top and bottom of Figure 51.…”