High-speed counter-current chromatography coupled online to high performance liquid chromatography–diode array detector–mass spectrometry for purification, analysis and identification of target compounds from natural products

Rapid screening and identification of antioxidants in the leaves of Malus hupehensis using off‐line two‐dimensional HPLC–UV–MS/MS coupled with a 1,1′‐diphenyl‐2‐picrylhydrazyl assay

“…Thus, the structures of compound 9 and 10 were tentatively established as phloretin‐2'‐xyloglucoside and phloretin‐2'‐xylopyranoside , respectively. Following the similar principle, compound 1 , 15 , 21 , 22 , and 23 were plausibly identified as 3‐hydroxyphloretin , 4',6'‐dihydroxydihydrochalcone‐2'‐ O ‐β‐ d ‐glucoside , 3,5‐dihydroxy phloridzin, 3‐hydroxy phloridzin , and trilobatin , respectively.…”

Separation of polar antioxidants from Rhizoma Polygonatum Odorati by high-speed counter-current chromatography with a hydrophilic solvent system

“…Up to now, there are two kinds of solvent systems, namely organic-aqueous and aqueous-aqueous systems. The conventional used former one (e.g., n-hexane-ethyl acetatemethanol-water, ethyl acetate-n-butanol-water, and nbutanol-water solvent system with different volume ratios) could isolate compounds with hydrophobic to moderate polarity, [16][17][18][19][20][21] but often fails to provide suitable K values for highly polar compounds. [22] The latter one (e.g., polyethylene glycol-inorganic salt and polyethylene glycol-dextran solvent system) is successfully applied to separate polar compounds, such as proteins and nucleic acids, however, the polymers are not easy to be eliminated from target compounds.…”

“…[16][17][18][19][20][21] Selection of applicable solvent system is essential for the provision of suitable partition coefficient (K) for the target compounds. Up to now, there are two kinds of solvent systems, namely organic-aqueous and aqueous-aqueous systems.…”

“…[23][24] Notably, a series of highly polar solvent systems, composed of hydrophilic organic solvent (e.g., ethanol and methanol) and inorganic salt solution, have recently been developed to purify highly polar compounds (e.g., catecholamines, basic dyes, sulfonic acids, zwitter ions, nucleotides, and their derivatives). [22,[24][25][26][27] As part of our ongoing efforts on isolation and evaluation of bioactive compounds from medicinal herbs, [6,7,13,[16][17][18] a hydrophilic solvent system, composed of n-butanol-ethanol-2 M ammonium sulfate aqueous solution, is firstly developed to isolate highly polar antioxidants from n-butanol fraction of Rhizoma Polygonatum Odorati target-guided by DPPH-HPLC assay. Followed by further purification on Sephadex LH-20 column chromatography, we yield nine highly polar antioxidants with purities over 98.0%, which include four nucleosides, cytidine (1), uridine (4), guanosine (5), and adenosine (8); two nucleobases, guanine (3) and adenine (6); and three amino acids, tyrosine (2), phenylalanine (7), and tryptophan (9).…”

Isolation of Herbicidal Substance, α-terthienyl, from the Root of Flaveria bidentis using HSCCC and HPLC

“…This method has been widely applied in the separation and preparation of natural products (Assimopoulou et al, 2009;Zhou et al, 2005;Liang et al, 2015). HSCCC by timecontrolled collection method has been successfully used in isolation and purification of the α-terthienyl from F. bidentis (L.) Kuntze.…”