High-speed in vivo calcium recording using structured illumination with self-supervised denoising
Forest Speed,
Catherine A. Saladrigas,
Alec Teel
et al.
Abstract:High-speed widefield fluorescence imaging of neural activity in vivo is fundamentally limited by fluctuations in recorded signal due to background contamination and stochastic noise. In this study, we show background and shot noise-reduced imaging of the ultrafast genetically encoded Ca2+ indicator GCaMP8f in CA1 pyramidal neurons using periodic structured illumination (SI) with computational image reconstruction. We implement what we believe to be a novel reconstruction method for data acquired using periodic… Show more
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