High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Method and Theory

Kuss, Sabine; Trinh, Dao; Danis, Laurence; Mauzeroll, Janine

doi:10.1021/acs.analchem.5b01268

Cited by 29 publications

(48 citation statements)

References 25 publications

(58 reference statements)

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“…The heterogeneous rate constant of this reaction at the microelectrode tip varies and is determined by numerical modeling, based on the previously established forced convection effect. 30 The recorded decrease of cellular metabolic activity confirms the beneficial effect of EGCg on cancer cells.…”

mentioning

confidence: 57%

“…HeLa cells (CCL-2, American Type Culture Collection, VA, USA) were cultured in Dulbecco's Modified Eagle's Medium (L-glutamine, 4500 mg/L glucose, and 110 mg/L sodium pyruvate), harvested, and seeded in Petri dishes following an established protocol as previously reported. 30 Cell Viability Measurements. HeLa cells were seeded in 15 × 60 mm Petri dishes (200 000 cells/dish) and incubated for 3 days at 37°C and 5% CO 2 .…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Application to Live Cell Imaging in Human Cancer

2015

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Scanning electrochemical microscopy (SECM) is increasingly applied to study and image live cells. Quantitative analyses of biological systems, however, still remain challenging. In the presented study, single human adenocarcinoma cervical cancer cells are electrochemically investigated by means of SECM. The target cell's electrochemical response is observed over time under the influence of green tea catechins (GTC), which are suggested to offer chemopreventive and therapeutic effects on cancer. The electrochemical response of living target cells is measured experimentally and quantified in an apparent heterogeneous rate constant by using a numerical model, based on forced convection during high speed SECM imaging. The beneficial effect of GTC on cancer cells could be confirmed by SECM, and the presented study shows an alternative approach toward unraveling the mechanisms involved during inhibition of carcinogenesis.

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confidence: 57%

Section: ■ Experimental Sectionmentioning

confidence: 99%

High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Application to Live Cell Imaging in Human Cancer

2015

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“…Identified through CV, a biased UME tip potential of 0.3 V for FcMeOH and 0.4 V for FcCOOH was used to obtain their steady state current. SECM analysis of all samples was performed at a maximum UME tip speed of 21.4 μm/s, to limit the effects of forced convection and maintain steady state current . SECM was performed on the desired cells using the depth scan imaging.…”

Section: Methodsmentioning

confidence: 99%

Tracking Live‐Cell Response to Hexavalent Chromium Toxicity by using Scanning Electrochemical Microscopy

Henderson

Filice

et al. 2017

ChemElectroChem

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The effects of exposure to toxic heavy metals, such as chromium, are of interest in scientific research, owing to its association with oxidative stress, cytotoxicity, and carcinogenicity. This study aims to explore the effects of Cr (VI) on live cell responses. Herein, scanning electrochemical microscopy (SECM) is employed by using depth scan imaging and feedback mode to monitor the membrane permeability of single live human bladder cancer (T24) cells following 1 h incubations with Cr (VI) stimuli. By using membrane‐permeable and impermeable redox mediators, ferrocenemethanol and ferrocenecarboxylic acid, respectively, SECM depth scans yield both electrochemical and topographic information. This provides insights into the relative changes in membrane homeostasis with increased exposure to Cr (VI). Here, SECM has shown great power in determining membrane response to Cr (VI) exposure. Dependent on the level of exposure, transition between three distinct trends was observed. At low incubation concentrations of Cr (VI), the cell membrane permeability coefficients were relatively unaffected. With moderate increases in Cr (VI) concentrations, membrane permeability coefficients of the incubated cells were observed to decrease. Finally, with the higher incubation concentrations, membrane permeability coefficients were found to increase toward values similar to control cells. The Cr (VI) toxicity was further investigated by means of a MTT cell viability study, which exhibited a similar decreasing trend to the cell membrane permeability. These findings further demonstrate the strength of SECM as a bioanalytical technique for monitoring cellular homeostasis.

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“…For SECM, the challenge of high-speed imaging remains the availability of models that take into account the effects of increased and forced convection, fluid flow and changes to diffusion, caused by the probe moving at increased velocities [120][121][122].…”

Section: Fast Scanning and Imaging Movies Obtained With Sepmmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby

White

2018

Chemosensors

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This review discusses a broad range of recent advances (2013)(2014)(2015)(2016)(2017) in chemical imaging using electrochemical methods, with a particular focus on techniques that have been applied to study cellular processes, or techniques that show promise for use in this field in the future. Non-scanning techniques such as microelectrode arrays (MEAs) offer high time-resolution (<10 ms) imaging; however, at reduced spatial resolution. In contrast, scanning electrochemical probe microscopies (SEPMs) offer higher spatial resolution (as low as a few nm per pixel) imaging, with images collected typically over many minutes. Recent significant research efforts to improve the spatial resolution of SEPMs using nanoscale probes and to improve the temporal resolution using fast scanning have resulted in movie (multiple frame) imaging with frame rates as low as a few seconds per image. Many SEPM techniques lack chemical specificity or have poor selectivity (defined by the choice of applied potential for redox-active species). This can be improved using multifunctional probes, ion-selective electrodes and tip-integrated biosensors, although additional effort may be required to preserve sensor performance after miniaturization of these probes. We discuss advances to the field of electrochemical imaging, and technological developments which are anticipated to extend the range of processes that can be studied. This includes imaging cellular processes with increased sensor selectivity and at much improved spatiotemporal resolution than has been previously customary.

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High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Method and Theory

Cited by 29 publications

References 25 publications

High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Application to Live Cell Imaging in Human Cancer

High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Application to Live Cell Imaging in Human Cancer

Tracking Live‐Cell Response to Hexavalent Chromium Toxicity by using Scanning Electrochemical Microscopy

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

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