High throughput circRNAs sequencing profile of follicle fluid exosomes of polycystic ovary syndrome patients

Abstract

Polycystic ovary syndrome (PCOS) is one of the most prevalent reproductive disorders in women worldwide. Despite rigorous research, the exact molecular mechanism that governs PCOS pathogenesis remains unclear. To investigate the potential roles of circular RNAs (circRNAs), this study sequenced ribosomal RNA‐depleted total RNA from exosomes of follicle fluids obtained from PCOS patients using non‐PCOS samples as controls. Bioinformatic analysis identified 167 upregulated and 245 downregulated circRNAs from a total of 16,771 detected candidates. Functional analysis suggests that pathways related to bacterial infection, associated chronic inflammation, and oxidative stress could be targeted by the differential circRNAs in PCOS patients. The obtained sequencing results were further validated by quantitative reverse‐transcription polymerase chain reaction and a circRNA–microRNA interaction network was constructed. The obtained results provide a valuable addition to the published studies on the mechanism of PCOS pathogenesis by revealing a wide variety of new circRNAs, miRNA, and gene targets that merit further investigation.

Exosomal circular RNA sorting mechanisms and their function in promoting or inhibiting cancer (Review)

“…Exosomal circRNAs promote cancer progression. circRNAs in exosomes derived from patients with multiple tumours are distinct from those of healthy individuals (88), indicating that they may have great clinical significances and research value. A number of studies have investigated the mechanism by which exosomal circRNAs function in cancer development.…”

Exosomes as a new approach into cell-to-cell communication within the mammalian ovary

“…folliculosome) dla określenia egzosomów pochodzących z płynu pęcherzykowego, co podkreśla specyficzne pochodzenie wyizolowanych struktur. Odkrycie egzosomów u zwierząt skłoniło naukowców także do badań nad ludzkimi pęcherzykami jajnikowymi [42,43,47]. W Tabeli 1 przedstawiono chronologicznie przykłady modeli doświadczalnych, u których izolowano egzosomy z płynu pęcherzykowego z uwzględnieniem pełnionych przez nie funkcji oraz metody izolacji.…”

“…infekcje bakteryjne, stres oksydacyjny czy autofagia. Badania te są podwaliną do dalszych analiz dotyczących roli tych cząsteczek w patogenezie PCOS [47]. Najnowsze prace odnoszą się także do roli egzosomów jako potencjalnych biomarkerów nowotworu jajnika.…”

Biogenesis and functions of circular RNAs and their role in diseases of the female reproductive system

“…The sequencing results were further verified by constructing the circRNA-miRNA network. Moreover, circRNA_103827 predicted the pregnancy outcome after assisted reproductive technology, indicating its potential as a new biomarker for predicting the outcome of in vitro fertilization embryo transfer [ 80 ].…”