High-throughput comprehensive analysis of d- and l-amino acids using ultra-high performance liquid chromatography with a circular dichroism (CD) detector and its application to food samples

Eto, Sachise; Yamaguchi, Mai; Bounoshita, Masao; Mizukoshi, Toshimi; Miyano, Hiroshi

doi:10.1016/j.jchromb.2011.07.025

Cited by 38 publications

(28 citation statements)

References 37 publications

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“…A new d-and l-AA analysis method was developed using a CD detector. A comprehensive analysis of d-and l-AAs carried out by ultra-HPLC circular dichroism (CD) detector after pre-column derivatization with NBD-F and its application to food samples was explored [145].…”

Section: -Dimensional Hplcmentioning

confidence: 99%

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Tanwar

Bhushan

2015

Chromatographia

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Section: -Dimensional Hplcmentioning

confidence: 99%

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Tanwar

Bhushan

2015

Chromatographia

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“…Eto et al . () used NBD‐F for the analysis of d,l ‐amino acids in food samples, where the NBD–amino acids were separated on a reversed‐phase column and detected by circular dichroism at 235 nm. Proteinogenic amino acid enantiomers were analyzed within 5 min where the concomitant use of a reversed‐phase column and a chiral column (two‐dimensional HPLC) was suitable for the sensitive determination of NBD–amino acid enantiomers.…”

Section: Development and Application Of Benzofurazan Derivatization Rmentioning

confidence: 99%

Recent advances in development and application of derivatization reagents having a benzofurazan structure: a brief overview

Santa

2014

Biomedical Chromatography

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Chemical derivatization is often used to improve the separation efficiency and to enhance the detectability of the target compounds in high-performance liquid chromatography and capillary electrophoresis. The derivatization reagents having a benzofurazan (2,1,3-benzoxadiazole) structure are one of the most often used reagent for this purpose. In this paper, the recent advances in the development and the application of benzofurazan derivatization reagents are reviewed.

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“…For instance, an achiral HPLC‐CD system can be used for ee measurements without chiral separation and thus shortens the time required for analysis. Additionally, an HPLC‐CD system makes it possible to simultaneously determine the ee values for many chiral compounds in a complex sample …”

Section: Introductionmentioning

confidence: 99%

Deviations from Beer's law in electronic absorption and circular dichroism: Detection for enantiomeric excess analysis

et al. 2019

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The electronic absorption (UV) to circular dichroism (CD) signal ratio can be used for enantiomeric excess (ee) analysis within linear range. However, CD detection often requires a high sample concentration where deviations from Beer's law may occur. Individual enantiomers of four chiral compounds were separated from commercial racemates by semipreparative high‐performance liquid chromatography (HPLC) with chiral columns. They were used to trace possible deviations in both UV and CD detection on achiral HPLC with a photodiode array detector and a CD detector. The CD/UV ratios for samples with the same ee value decreased by up to 7.8 to 52% when the injection volume increased, indicating that the linear standard curve of ee versus CD/UV is only valid within a narrow range. To extend the sample amount to a wider range, a data‐processing method was developed based on two second‐order polynomial functions, which were constructed to fit the relationship between the intensities of the UV and CD signals for two enantiomers. Moreover, a more simplified method based on a third‐order polynomial function was established to calculate the ee values. The variations between the predicted and experimental ee values were within ±0.08 for both methods. To our knowledge, this is the first study that the deviations from Beer's law are considered in both UV and CD detection for ee analysis.

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High-throughput comprehensive analysis of d- and l-amino acids using ultra-high performance liquid chromatography with a circular dichroism (CD) detector and its application to food samples

Cited by 38 publications

References 37 publications

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Recent advances in development and application of derivatization reagents having a benzofurazan structure: a brief overview

Deviations from Beer's law in electronic absorption and circular dichroism: Detection for enantiomeric excess analysis

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