High‐Throughput Evaluation of Antioxidant and Pro‐oxidant Activities of Polyphenols with Thymidine Protection Assays

Meunier, Stéphane; Hanedanian, Mikael; Murr, Marine Desage‐El; Nowaczyk, Stéphanie; Gall, Thierry Le; Pin, Serge; Renault, Jean Philippe; Boquet, Didier; Créminon, Christophe; Mioskowski, Charles; Taran, Frédéric

doi:10.1002/cbic.200400421

Cited by 32 publications

(38 citation statements)

References 36 publications

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“…27 Firstly, the protection of thymidine towards three types of oxidative conditions (c-rays, UV/H 2 O 2 , and Fenton oxidation) was determined using the previously described immuno-enzymatic assay. 10,13 Then, the radical scavenging properties of the compounds were studied using assays based on two oxidants, the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH Å ) and the radical cation of (2,2 0 -azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS Å+ ). Finally the evaluation of b-carotene protection in a lipidic emulsion under oxidative conditions by the compounds was realized.…”

Section: Antioxidant Assaysmentioning

confidence: 99%

“…However, this product was found to be inefficient towards another oxidative stress, the Fenton-type oxidation, which was instead rather typical of a pro-oxidant activity. 13 Further studies showed that a structurally related pigment, di-O-methylatromentic acid (2), efficiently protected thymidine towards the three stress conditions, and did not show any pro-oxidant behavior. Compound 2 belongs to a larger pigment family typical of boletes, and also found in lichens, pulvinic acids, the simpler of which is pulvinic acid (3).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and antioxidant properties of pulvinic acids analogues

Nadal

Thétiot-Laurent

et al. 2010

Bioorganic & Medicinal Chemistry

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Section: Antioxidant Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and antioxidant properties of pulvinic acids analogues

Nadal

Thétiot-Laurent

et al. 2010

Bioorganic & Medicinal Chemistry

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“…They have been demonstrated to function as antioxidants in vitro both in cell cultures and cell-free systems by scavenging superoxide anion, singlet oxygen, lipid peroxy-radicals, and/or stabilizing free radicals involved in oxidative proc-esses through hydrogenation or complexing with oxidizing species [3,18,19]. However, in vitro experiments reported that most of dietary compounds with therapeutical properties act as potent pro-oxidants molecules [20,21] at high concentrations or in presence of transition metals [22]. This pro-oxidant effect increases reactive oxygen species (ROS) production which, acting as second messengers of signaling networks, may induce growth arrest and apoptosis [23].…”

Section: Introductionmentioning

confidence: 96%

Quercetin‐induced apoptotic cascade in cancer cells: Antioxidant versus estrogen receptor α‐dependent mechanisms

Galluzzo

Martini

Bulzomi

et al. 2009

Molecular Nutrition Food Res

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The flavonol quercetin, especially abundant in apple, wine, and onions, is reported to have anti-proliferative effects in many cancer cell lines. Antioxidant or pro-oxidant activities and kinase inhibition have been proposed as molecular mechanisms for these effects. In addition, an estrogenic activity has been observed but, at the present, it is poorly understood whether this latter activity plays a role in the quercetin-induced anti-proliferative effects. Here, we studied the molecular mechanisms of quercetin committed to the generation of an apoptotic cascade in cancer cells devoid or containing transfected estrogen receptor alpha (ERalpha; i.e., human cervix epitheloid carcinoma HeLa cells). Although none of tested quercetin concentrations increase reactive oxygen species (ROS) generation in HeLa cells, quercetin stimulation prevents the H(2)O(2)-induced ROS production both in the presence and in the absence of ERalpha. However, this flavonoid induces the activation of p38/MAPK, leading to the pro-apoptotic caspase-3 activation and to the poly(ADP-ribose) polymerase cleavage only in the presence of ERalpha. Notably, no down-regulation of survival kinases (i.e., AKT and ERK) was reported. Taken together, these findings suggest that quercetin results in HeLa cell death through an ERalpha-dependent mechanism involving caspase- and p38 kinase activation. These findings indicate new potential chemopreventive actions of flavonoids on cancer growth.

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“…Previous studies have indicated that some nutraceuticals exhibit potent anti-tumor properties and can modulate apoptosis, differentiation and cell cycle, probably by virtue of their anti-oxidant functions. However, in vitro experiments reported that most of them behave as potent pro-oxidants molecules [6,9,40]. For example, organosulfur molecules from garlic, such as the oil-soluble diallyl disulfide [12,13], diallyl trisulfide [22], or the water-soluble S-allylmercaptocysteine [63] have been demonstrated to induce growth arrest and apoptosis dose-dependently by increasing ROS production.…”

Section: Introductionmentioning

confidence: 99%

trans-Resveratrol induces apoptosis in human breast cancer cells MCF-7 by the activation of MAP kinases pathways

et al. 2007

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Polyphenols represent a large class of plantderived molecules with a general chemical structure that act as potent free radical scavengers. They have long been recognized to possess several therapeutic activities ranging from anti-thrombotic to antioxidant. Moreover, the capability of polyphenols to act as reducing or oxidizing molecules depends on the presence of environmental metals and on the concentrations used. In this work we demonstrated that the stilbene trans-resveratrol was able to commit human breast cancer MCF-7 cells to apoptosis. Mainly, we evidenced a pivotal role of the mitochondria in this phenomenon as cytochrome c release into the cytosol was found after the treatment. We further showed that trans-resveratrol was able to affect cellular redox state. In particular, it induced an early production of ROS and lipid oxidation, and only later compromised the GSH/GSSG ratio. This mode of action was mirrored by a temporally different activation of JNK and p38 MAPK , with the former rapidly induced and the latter weakly activated at long intervals. The results obtained demonstrate a pro-apoptotic activity for trans-resveratrol, and suggest a preferential activation of different classes of MAP kinases in response to different oxidative stimuli (ROS versus GSH/GSSG alteration).

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High‐Throughput Evaluation of Antioxidant and Pro‐oxidant Activities of Polyphenols with Thymidine Protection Assays

Cited by 32 publications

References 36 publications

Synthesis and antioxidant properties of pulvinic acids analogues

Synthesis and antioxidant properties of pulvinic acids analogues

Quercetin‐induced apoptotic cascade in cancer cells: Antioxidant versus estrogen receptor α‐dependent mechanisms

trans-Resveratrol induces apoptosis in human breast cancer cells MCF-7 by the activation of MAP kinases pathways

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