2023
DOI: 10.1021/acsomega.2c06577
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High-Throughput Exonuclease Assay Based on the Fluorescent Base Analogue 2-Aminopurine

Abstract: Exonucleases are essential enzymes that remove nucleotides from free DNA ends during DNA replication, DNA repair, and telomere maintenance. Due to their essential role, they are potential targets for novel anticancer and antimicrobial drugs but have so far been little exploited. Here, we present a simple and versatile real-time exonuclease assay based on 2-aminopurine, an intrinsically fluorescent nucleotide that is quenched by neighboring bases when embedded in DNA. We show that our assay is applicable to dif… Show more

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