Abstract:Exonucleases are essential enzymes that remove nucleotides
from
free DNA ends during DNA replication, DNA repair, and telomere maintenance.
Due to their essential role, they are potential targets for novel
anticancer and antimicrobial drugs but have so far been little exploited.
Here, we present a simple and versatile real-time exonuclease assay
based on 2-aminopurine, an intrinsically fluorescent nucleotide that
is quenched by neighboring bases when embedded in DNA. We show that
our assay is applicable to dif… Show more
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