High-throughput gene-expression quantification of grapevine defense responses in the field using microfluidic dynamic arrays

Dufour, Marie‐Cécile; Magnin, Noël; Dumas, Bernard; Vergnes, Sophie; Corio‐Costet, Marie‐France

doi:10.1186/s12864-016-3304-z

Cited by 34 publications

(40 citation statements)

References 62 publications

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“…PE changed the expression of all studied defense-related genes, which is in common with other documented elicitors ( Aziz et al, 2007 ; Lachhab et al, 2014 ; Dufour et al, 2016 ). Interestingly, using cell suspensions, we showed that PE simultaneously upregulated the expression of 9-LOX and repressed 13-LOX one.…”

Section: Discussionsupporting

confidence: 70%

A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

et al. 2018

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Protecting vineyards from cryptogamic diseases such as downy mildew, caused by Plasmopara viticola, generally requires a massive use of phytochemicals. However, the issues on unintentional secondary effects on environment and human health, and the occurrence of P. viticola resistant strains, are leading to the development of alternative strategies, such as the use of biocontrol products. In this paper, we evidenced the ability of a plant extract to protect grapevine from P. viticola. Further experiments carried out both on cell suspensions and on plants revealed that plant extract activates typical defense-related responses such as the production of H2O2, the up-regulation of genes encoding pathogenesis-related proteins and stilbene synthase, as well as the accumulation of resveratrol or its derivative piceid. We also brought to light a strong direct effect of PE on the release and motility of P. viticola zoospores. Furthermore, we found out that PE application left dried residues on leaf surface, impairing zoospores to reach stomata. Altogether, our results highlight the different modes of action of a new biocontrol product able to protect grapevine against downy mildew.

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Section: Discussionsupporting

confidence: 70%

A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

et al. 2018

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“…Several defense reactions of the plant were observed and measured after PP treatment, for example, papilla formation, lignification, ethylene biosynthesis, phenylalanine ammonia lyase activation, or accumulation of phytoalexins [ 17 ], but have never been analyzed in berries in detail. However, grapevines treated with the phosphonate fosetyl-aluminum showed only a very weak induction of defense-related genes [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Together for the Better: Improvement of a Model Based Strategy for Grapevine Downy Mildew Control by Addition of Potassium Phosphonates

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et al. 2020

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Grapevine downy mildew is one of the major diseases in viticulture. To control this disease, a more effective strategy has been developed and established based on growth and model data as well as on a combination of fungicides. For this purpose, the systemic plant protection product potassium phosphonate (PP) was combined with two contact fungicides. Treatments were carried out according to the different experimental conditions after the growth of 400 cm2, 600 cm2, and 800 cm2 leaf area per primary shoot. PP increased the effectiveness of the preventive fungicides whenever high infection pressure was the case. The experiments also show that it is possible to extend the treatment intervals from 400 cm2 to 600 cm2 new leaf area when PP was added. However, none of the tested treatments were sufficient for the extension to intervals of 800 cm2. These data show that PP can be a key factor in the reduction of the application of synthetic or copper-based fungicides.

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“…Grapevines have very dynamic gene expression responses to pathogens [70,71]. The top 150 DEGs for BOD berries were highly enriched with biotic stress genes.…”

Section: Transcript Abundance Of Biotic Stress Genesmentioning

confidence: 99%

A sense of place: transcriptomics identifies environmental signatures in Cabernet Sauvignon berry skins in the late stages of ripening

et al. 2020

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Background: Grape berry ripening is influenced by climate, the main component of the "terroir" of a place. Light and temperature are major factors in the vineyard that affect berry development and fruit metabolite composition. Results: To better understand the effect of "place" on transcript abundance during the late stages of berry ripening, Cabernet Sauvignon berries grown in Bordeaux and Reno were compared at similar sugar levels (19 to 26°Brix (total soluble solids)). Day temperatures were warmer and night temperatures were cooler in Reno.°Brix was lower in Bordeaux berries compared to Reno at maturity levels considered optimum for harvest. RNA-Seq analysis identified 5528 differentially expressed genes between Bordeaux and Reno grape skins at 22°Brix. Weighted Gene Coexpression Network Analysis for all expressed transcripts for all four°Brix levels measured indicated that the majority (75%) of transcript expression differed significantly between the two locations. Top gene ontology categories for the common transcript sets were translation, photosynthesis, DNA metabolism and catabolism. Top gene ontology categories for the differentially expressed genes at 22°Brix involved response to stimulus, biosynthesis and response to stress. Some differentially expressed genes encoded terpene synthases, cell wall enzymes, kinases, transporters, transcription factors and photoreceptors. Most circadian clock genes had higher transcript abundance in Bordeaux. Bordeaux berries had higher transcript abundance with differentially expressed genes associated with seed dormancy, light, auxin, ethylene signaling, powdery mildew infection, phenylpropanoid, carotenoid and terpenoid metabolism, whereas Reno berries were enriched with differentially expressed genes involved in water deprivation, cold response, ABA signaling and iron homeostasis. Conclusions: Transcript abundance profiles in the berry skins at maturity were highly dynamic. RNA-Seq analysis identified a smaller (25% of total) common core set of ripening genes that appear not to depend on rootstock, vineyard management, plant age, soil and climatic conditions. Much of the gene expression differed between the two locations and could be associated with multiple differences in environmental conditions that may have affected the berries in the two locations; some of these genes may be potentially controlled in different ways by the vinegrower to adjust final berry composition and reach a desired result.

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High-throughput gene-expression quantification of grapevine defense responses in the field using microfluidic dynamic arrays

Cited by 34 publications

References 62 publications

A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

Together for the Better: Improvement of a Model Based Strategy for Grapevine Downy Mildew Control by Addition of Potassium Phosphonates

A sense of place: transcriptomics identifies environmental signatures in Cabernet Sauvignon berry skins in the late stages of ripening

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