High-Throughput Injection System for Zebrafish Fertilized Eggs

Abstract

The zebrafish have been successfully become a popular vertebrate model for studying gene function by largescale genetic screens due to the see-through embryos and their accessibility to various experimental techniques throughout development. Furthermore, whole genomic sequence of the zebrafish genome has completely finished and its relationship to the human genomic data became clearer. The request of technological development in experiment using zebrafish must be accelerated with the increasing demand of zebrafish for understanding of the roles of specific genes involving human diseases. Here, we introduced a high-throughput injection system for zebrafish fertilized eggs to overcome the problems caused by manual injection and proposed a unique utilization of zebrafish as a host vector system of protein expression, not as a vertebrate models.