High throughput microRNAs sequencing profile of serum exosomes in women with and without polycystic ovarian syndrome

Zhang, Feng; Li, Suping; Zhang, Tao; Zhang, Juan; Ding, Haigang; Ye, Feijun; Yuan, Hua; Ma, Yingying; Pan, Haitian; He, Yao

doi:10.7717/peerj.10998

Cited by 17 publications

(17 citation statements)

References 31 publications

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“…Samples were sieved using 0.22-μm-pore-size filters following a method describedpreviously [ 13 ]. After precipitation of exosomes with the ExoQuick Exosome Precipitation Solution (System Biosciences-SBI), they were analyzed using an electron microscope (FEI Quanta 250, Thermo Fisher Scientific, Eindhoven, the Netherlands), the Nano particle tracking analysis (NTA) (ZetaView PMX 110, Particle Metrix, Meerbusch, Germany), and Western blot analysis (for CD63) were used to characterize isolated exosomes.…”

Section: Methodsmentioning

confidence: 99%

Profiling of exosomal microRNAs expression in umbilical cord blood from normal and preeclampsia patients

Pan

Shi

Fang

et al. 2022

BMC Pregnancy Childbirth

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Background Epidemiological and experimental studies suggest that preeclampsia has a negative impact on maternity and offspring health. Previous studies report that dysregulation in utero-environment increases risk for elderly disease such as cardiovascular disease. However, the underlying mechanisms remain elusive. Specific microRNAs (miRNAs) are packaged in exosomes may regulate microvascular dysfunction in offspring of mothers with preeclampsia. The present study aimed to identify the differential expression profiles of microRNAs in the serum exosomes between patients with preeclampsia and normal pregnancies. Methods A comprehensive miRNA sequence-based approach was performed to compare exosomes carry miRNAs (Exo-miRNAs) expression levels in umbilical serum between normal and preeclampsia patients. Exosomes were isolated using the ExoQuick precipitation kit. Serum exosomes were then viewed under electron microscopy, and their characteristics determined by western blotting and nanoparticle-tracking analysis. Illumina platform was used to perform sequencing. Bioinformatics analysis was used to explore differentially expressed Exo-miRNAs in umbilical serum. Results Based on sequence similarity, 1733 known miRNAs were retrieved. Furthermore, 157 mature miRNAs in serum exosomes were significantly differential expressed between PE and those control groups (P<0.05, log2|FC| > 1). Out, of the 157 miRNAs, 96 were upregulated miRNAs whereas 61 miRNAs were downregulated. The 157 differentially expressed miRNAs targeted 51,424 differentially expressed genes. Functional analysis through KEGG pathway and Gene Ontology results uncovered that target genes of miRNAs with differential expression were significantly linked to several pathways and biological processes. Conclusion The findings of this study showed differential expression of umbilical serum Exo-miRNAs in normal compared with PE patients, implying that these Exo-miRNAs may associate with microvascular dysfunction in offspring of mothers with preeclampsia.

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Section: Methodsmentioning

confidence: 99%

Profiling of exosomal microRNAs expression in umbilical cord blood from normal and preeclampsia patients

Pan

Shi

Fang

et al. 2022

BMC Pregnancy Childbirth

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“…[74, [90][91][92][93][94] ultimately may lead to an early intervention. Recent studies have found that cargo from follicle-derived exosomes includes various PCOS-specific miRNAs such as miR-373, miR-640, and miR-654-5p, with newer biomarkers being hsa-miR-1299, hsa-miR-6818-5p hsa-miR-192-5p, and hsa-miR-145-5p [66,67]. These specific miRNA biomarkers have been found to be overexpressed in PCOS patients and may possibly be used as diagnostic tools [67].…”

Section: Pre-eclampsiamentioning

confidence: 99%

“…Recent studies have found that cargo from follicle-derived exosomes includes various PCOS-specific miRNAs such as miR-373, miR-640, and miR-654-5p, with newer biomarkers being hsa-miR-1299, hsa-miR-6818-5p hsa-miR-192-5p, and hsa-miR-145-5p [66,67]. These specific miRNA biomarkers have been found to be overexpressed in PCOS patients and may possibly be used as diagnostic tools [67]. Furthermore, another recent study found that serum exosomes from PCOS patients expressed highly elevated levels of miR-590-3p and miR-27a-5p [62].…”

Section: Pre-eclampsiamentioning

confidence: 99%

“…The dysregulation of such constituents can also point to the strategy of loading these particles into exosomes to act as therapeutic cargo for targeted delivery, where specific types of molecules such as miRNA or proteins could mediate dysregulated pathways. Thus, the miRNA exosomal markers may have the potential to provide a basis for the early diagnosis of PCOS and understanding precipitating factors which may also promote the development of endometrial cancer from PCOS [62,[66][67][68][69][70][71].…”

Section: Pre-eclampsiamentioning

confidence: 99%

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The exosome: a review of current therapeutic roles and capabilities in human reproduction

Dimik

Abeysinghe

Logan

et al. 2022

Drug Deliv. and Transl. Res.

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Exosomes are nano-vesicles (30–150 nm) which may be useful as therapeutic delivery vehicles and as diagnostic biomarkers. Exosomes are produced naturally within the human body and therefore are not prone to immunogenicity effects which would otherwise destroy unelicited foreign bodies. Clinically, they have been regarded as ideal candidates for applications relating to biomarker developments for the early detection of different diseases. Furthermore, exosomes may be of interest as potential drug delivery vehicles, which may improve factors such as bioavailability of loaded molecular cargo, side effect profiles, off-target effects, and pharmacokinetics of drug molecules. In this review, the therapeutic potential of exosomes and their use as clinical biomarkers for early diagnostics will be explored, alongside exosomes as therapeutic delivery vehicles. This review will evaluate techniques for cargo loading, and the capacity of loaded exosomes to improve various reproductive disease states. It becomes important, therefore, to consider factors such as loading efficiency, loading methods, cell viability, exosomal sources, exosome isolation, and the potential therapeutic benefits of exosomes. Issues related to targeted drug delivery will also be discussed. Finally, the variety of therapeutic cargo and the application of appropriate loading methods is explored, in the context of establishing clinical utility. Graphical abstract Exosomes have more recently been widely accpeted as potential tools for disease diagnostics and the targeted delivery of certain therapeutic molecules–and in due time exosomes will be utilised more commonly within the clinical setting. Specifically, exosomal biomarkers can be identified and related to various detrimental conditions which occur during pregnancy. Considering, this review will explore the potential future of exosomes as both diagnostic tools and therapeutic delivery vehicles to treat related conditions, including the challenges which exist towards incorporating exosomes within the clinical environment to benefit patients.

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“…Samples were sieved using 0.22-µm-pore-size lters following a method describedpreviously 14 . After precipitation of exosomes with the ExoQuick Exosome Precipitation Solution (System Biosciences-SBI), they were analyzed using an electron microscope (FEI Quanta 250, Thermo Fisher Scienti c, Eindhoven, the Netherlands), the Nano particle tracking analysis (NTA) (ZetaView PMX 110, Particle Metrix, Meerbusch, Germany), and Western blot analysis (for CD63) were used to characterize isolated exosomes.…”

Section: Exosomes Puri Cation and Characterizationmentioning

confidence: 99%

Profiling of Exosomal MicroRNAs Expression in Umbilical Cord Blood from Normal and Preeclampsia Patients

Pan

Shi

Fang

et al. 2021

Preprint

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Background: Epidemiological and experimental studies suggest that preeclampsia has a negative impact on maternity and offspring health. Previous studies report that dysregulation in utero-environment increases risk for elderly disease such as cardiovascular disease. However, the underlying mechanisms remain elusive. Specific microRNAs (miRNAs) are packaged in exosomes may regulate processes in offspring microvascular dysfunction.Methods: A comprehensive miRNA sequence-based approach to compare exosomes carry miRNAs (Exo-miRNAs) expression levels in umbilical serum between normal and preeclampsia patients was performed to explore mechanisms for development of cardiovascular dysfunction in offspring exposed to preeclampsia. by ExoQuick precipitations were used to isolate serum exosomes. Serum exosomes were then viewed under electron microscopy, and their characteristics determined by western blotting and nanoparticle-tracking analysis. Illumina platform was used to perform sequencing. Bioinformatics analysis was used to explore differentially expressed Exo-miRNAs in umbilical serum.Results: Based on sequence similarity, 1733 known miRNAs were retrieved. Furthermore, 157 mature miRNAs in serum exosomes were significantly differential expressed between PE and those control groups (P＜0.05, log2|FC|>1). Out, of the 157 miRNAs, 96 were upregulated miRNAs whereas 61 miRNAs were downregulated. The 157 differentially expressed miRNAs targeted 51424 differentially expressed genes. Functional analysis through KEGG pathway and Gene Ontology results uncovered that target genes of miRNAs with differential expression were significantly linked to several pathways and biological processes.Conclusion: The findings of this study showed differential expression of umbilical serum Exo-miRNAs in normal compared with PE patients, implying that these Exo-miRNAs play an important function in offspring microvascular dysfunction of preeclampsia.

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High throughput microRNAs sequencing profile of serum exosomes in women with and without polycystic ovarian syndrome

Cited by 17 publications

References 31 publications

Profiling of exosomal microRNAs expression in umbilical cord blood from normal and preeclampsia patients

Profiling of exosomal microRNAs expression in umbilical cord blood from normal and preeclampsia patients

The exosome: a review of current therapeutic roles and capabilities in human reproduction

Profiling of Exosomal MicroRNAs Expression in Umbilical Cord Blood from Normal and Preeclampsia Patients

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