High Throughput Screening of Glycopolymers: Balance between Cytotoxicity and Antibacterial Property

Abstract

To search for synthetic agents with low cytotoxicity and good antibacterial activity is essential for antimicrobial applications. Here we report a high throughput technique that carried out in multiwell plates via recyclable-catalyst-aided, opened-to-air, and sunlight-photolyzed RAFT (ROS-RAFT) polymerization. By using this method, three key monomers (MAG the sugar unit, DMAPMA the positively charged monomer, and DEMAA the hydrophobic monomer) can be polymerized in a controlled manner to afford glycopolymers. This simple high throughput technology is used to synthesize glycopolymers with variable compositions. The bacterial adhesion/killing ability and cytotoxicity of synthesized polymers have been evaluated, and glycopolymers with certain composition can achieve a balance of low cytotoxic and good antibacterial activity.

Aqueous broadband photopolymerization on microreactor arrays: from high throughput polymerization to fabricating artificial cells

“…The potential of the open-to-air sunlight polymerization in various applications is demonstrated. In the first example, we carried out high throughput polymerization to synthesize poly-mers with different monomer compositions for bacterial adhesion 33 (Fig. 5a).…”

New Light in Polymer Science: Photoinduced Reversible Addition-Fragmentation Chain Transfer Polymerization (PET-RAFT) as Innovative Strategy for the Synthesis of Advanced Materials

“…Gaojian Chen group [ 119 ] reported a recyclable-catalyst aided sunlight-photolyzed RAFT polymerization carried out in multiwell plates. High throughput technology afforded the synthesis of glycopolymers composed of hydrophobic, cationic, and sugar repeating units with variable compositions; their antibacterial activity and cytotoxicity were easily tested.…”

Plant Antimicrobial Peptides as Potential Tool for Topic Treatment of Hidradenitis Suppurativa

“…Although this methodology is quite robust, it can generate peptides without membrane specificity. Besides, a newly generated peptide may even present a different three-dimensional structure and higher cytotoxicity ( Zheng et al, 2019 ).…”

“…Although this methodology is quite robust, it can generate peptides without membrane specificity. Besides, a newly generated peptide may even present a different three-dimensional structure and higher cytotoxicity (Zheng et al, 2019). -Synthetic combinatory library: it is a powerful and widely used tool to obtain optimized classes of active peptides quickly.…”