High Throughput SNP Detection System Based on Magnetic Nanoparticles Separation

Abstract

Single-nucleotide polymorphism (SNP) was one-base variations in DNA sequence that can often be helpful to find genes associations for hereditary disease, communicable disease and so on. We developed a high throughput SNP detection system based on magnetic nanoparticles (MNPs) separation and dual-color hybridization or single base extension. This system includes a magnetic separation unit for sample separation, three high precision robot arms for pipetting and microtiter plate transferring respectively, an accurate temperature control unit for PCR and DNA hybridization and a high accurate and sensitive optical signal detection unit for fluorescence detection. The cyclooxygenase-2 gene promoter region −765G > C polymorphism locus SNP genotyping experiment for 48 samples from the northern Jiangsu area has been done to verify that if this system can simplify manual operation of the researchers, save time and improve efficiency in SNP genotyping experiments. It can realize sample preparation, target sequence amplification, signal detection and data analysis automatically and can be used in clinical molecule diagnosis and high throughput fluorescence immunological detection and so on.

Chelerythrine and Fe3O4 Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

“…Functionalization of the CNTs with magnetic Fe 3 O 4 nanoparticles (NPs) has been suggested to be very important in developing the targeting ability of the oxidized-CNTs. The multifunctional properties of the Fe 3 O 4 NPs, such as small size, high magnetic responsivity and low toxicity, [34][35][36][37][38][39][40][41][42][43] can be used to increase the accumulation of drugs in pathological sites, thereby reducing the non-specific toxicity and some other adverse side-effects. We therefore decorated the MWNTs with Fe 3 O 4 NPs to ensure a strong magnetic responsiveness and provide the targeted property for various applications.…”

Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

“…[14][15][16] Our group, in 2013, had presented a high-throughput SNP (Single Nucleotide Polymorphism) detection system to improve the efficiency in SNP genotyping. 17 Furthermore, a permanent magnet array was introduced into this system to act as the main executive supporter for magnetic nucleic acid separation. Generally, most of the automated workstations are mainly equipped with a robotic pipetting arm, a microtiter plate transferring grasper, a permanent magnet array module, some functional modules Copyright: American Scientific Publishers (e.g., shaker, heater and cooler, incubator, washer), and various types of labware adapters.…”

Development and Validation of an Automated Liquid Handling System for Sample Preparation Based on Multichannel Air Displacement Pipetting Technology

“…[7][8][9][10][11][12] High-throughput liquid handling technology is highly needed in life science laboratories because of its ability to process samples rapidly and tirelessly, especially in those integrated devices or instruments for high-throughput bioassays with the help of nanotechnology. [13][14][15][16][17][18][19][20][21][22][23][24][25][26] With advances in nanotechnology, [27][28][29][30][31][32][33][34][35][36][37] particularly in magnetic * Authors to whom correspondence should be addressed. nanotechnology, [38][39][40][41][42][43][44][45][46][47][48][49] high-throughput liquid handling technology can greatly increase the throughput of a laboratory and save precious time for scientists.…”