High-throughput system for screening of Monascus purpureus high-yield strain in pigment production

Tan, Jun; Chu, Ju; Wang, Yonghong; Zhuang, Yingping; Zhang, Siliang

doi:10.1186/s40643-014-0016-6

Cited by 13 publications

(6 citation statements)

References 20 publications

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“…The development of high-throughput screening technologies leads to wide applications in strain screening. Tan et al (2014) used a novel and effective mixture culture method, and performed a high-throughput screening of realized the whole mutant library of Monascus purpureus. An integrated strategy for high-throughput screening of high l-lactic acid-productivity strains in deep-well microtiter plates (MTPs) was developed by Lv et al (2016).…”

Section: Discussionmentioning

confidence: 99%

“…High-throughput screening technologies playa key role in reaching for improved strains, which is large scale, low cost, and high specificity (Ottenheim et al 2018;Zhang et al 2018). Highthroughput screening technologies consist in generating a mutant library in high-throughput miniaturized cultivation platforms, such as microplates (Tan et al 2013), shake flasks, micro-bioreactors (Tian et al 2018), and using a high-throughput detection platform (Tan et al 2014;Lv et al 2016). Unlike previous reported studies, which only focused on single target of product titer or productivity, the present study characterized two indexes for the production of high SLs contents by coutilizing glucose and glycerol capacity.…”

Section: Introductionmentioning

confidence: 99%

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Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity

Lin

Yang

et al. 2019

Bioresour. Bioprocess.

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Background: A rational and high-performance high-throughput screening system effectively improves the efficiency of strain screening. Results: A rapid and efficient method was developed to detect carbon sources. And then by using glucose analog as screening pressure and two indexes for sophorolipids (SLs) production and glycerol consumption, a mutant of Candida bombicola L1.3 was successfully selected among 3000 mutants. It produces high SLs titer and co-utilizes glucose and glycerol simultaneously. Conclusions: Compared to the wild-type strain, L1.3 exhibited 15.06% higher SLs titer and 35.69% higher glycerol consumption capacity in a 5-L bioreactor. We believe that L1.3 can potentially be used for the efficient industrial production of SLs with simple downstream processing for separating SLs and glycerol.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity

Lin

Yang

et al. 2019

Bioresour. Bioprocess.

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“…The special characteristic of Monascus is its ability to produce pigments so that when grown on agar slant, the mycelia that are initially white will turn yellow, orange, and then red. A high throughput screening system to determine a high pigments-producing strain of Monascus purpureus has been developed (Tan et al 2014 ). Research on the genetic and genomic aspects of the Monascus has long since been carried out.…”

Section: Monascus Fungimentioning

confidence: 99%

Utilization of agro-industrial by-products in Monascus fermentation: a review

Srianta

Kusdiyantini

Zubaidah

et al. 2021

Bioresour. Bioprocess.

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The Monascus fermentation industry has gained global attention. Its key products, i.e., pigments, functional food ingredients, food supplements, and medicinal use, are growing in the world’s market. Efforts to find the cost-effective substrate for Monascus fermentation have remained the target. This paper aimed to appraise the utilization of agro-industrial by-products (cereal, starchy tuber and root, legume, fruit, and coffee processing) as a cost-effective substrate for Monascus fermentation. The specific objective was to review the by-products pre-treatment, the fermentation process, product yield, and the bioactivity of the fermented products. Among all the by-products that could be used as the fermentation substrate, cereal brans do not need pre-treatment, but others need a suitable pre-treatment step, e.g., cassava peel, okara, and jackfruit seed to list a few, that need to be powdered beforehand. Other substrates, such as corn cob and durian seed, need soaking and size reduction through the pre-treatment step. During fermentation, Monascus produce many pigments, monacolin K, associated with rise in phenolic and flavonoid contents. These products possess antioxidant, antihypercholesterol, antidiabetes, and antiatherosclerosis activities which underpin their health significance. In conclusion, we report in this review the agro-industrial by-products which have potential prospects for pigments, functional food ingredients, food supplements, and therapeutic usages produced from Monascus fermentation.

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“…Microbial mutation breeding by altering the genomes shows great potential for biotechnology research and applications (Kumar et al 2015;Tan et al 2014). Microbial breeding mutagenesis can be achieved using various approaches, mainly physical, chemical and biological methods (Guo 2018).…”

Section: Introductionmentioning

confidence: 99%

Enhancing wuyiencin productivity of Streptomyces albulus (CK15) by mutagenesis breeding with atmospheric and room temperature plasma

Thein

Shi

Liu

et al. 2023

World J Microbiol Biotechnol

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Streptomyces species are commonly used for e cient secondary metabolite production, including many antibiotics. Wuyiencin is a secondary metabolite produced by Streptomyces albulus CK15. This antibiotic is used in agriculture for controlling various fungal diseases of vegetables and eld crops. In the present study, we used atmospheric and room temperature plasma (ARTP) mutagenesis to generate mutant S. albulus strains with the capacity for improved fermentation production of wuyiencin. The wild-type S. albulus CK15 strain was mutagenized by ARTP for one time and screened for two rounds of antimicrobial activity, three genetically stable mutants (M19, M26, and M28) were identi ed, which showed increased wuyiencin production by 17.41%, 13.57% and 18.46% compared to the CK15 strain in ask culture, respectively. The M28 mutant exhibited a maximum wuyiencin activity of 1442.95±134.61 U/mL in ask culture and 1673.84±127.42 U/mL in a 5 L fermenter. Therefore, our results provide evidence that ARTP is an e cient tool for microbial mutation breeding and the enhancement of wuyiencin production.

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High-throughput system for screening of Monascus purpureus high-yield strain in pigment production

Cited by 13 publications

References 20 publications

Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity

Rational high-throughput screening system for high sophorolipids production in Candida bombicola by co-utilizing glycerol and glucose capacity

Utilization of agro-industrial by-products in Monascus fermentation: a review

Enhancing wuyiencin productivity of Streptomyces albulus (CK15) by mutagenesis breeding with atmospheric and room temperature plasma

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