High transfection efficiency promoted by tailor-made cationic tri-block copolymer-based nanoparticles

Cordeiro, Rosemeyre A.; Santo, Daniela; Farinha, Dina; Serra, Arménio C.; Faneca, Henrique; Coelho, Jorge F. J.

doi:10.1016/j.actbio.2016.10.015

Cited by 29 publications

(33 citation statements)

References 33 publications

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“…The vast availability of monomers, the ease of synthesis, and the possibility to control parameters such as the molecular weight of the polymer, allows the design of countless different polymeric structures with different architectures and properties. Therefore, it is possible to create tailor-made polymers that attend the desired characteristics, such as hydrophilicity or hydrophobicity, surface charge or even responsiveness to certain stimuli [ 63 , 64 ].…”

Section: Hybrid Silica Nanoparticlesmentioning

confidence: 99%

Section: Hybrid Silica Nanoparticlesmentioning

confidence: 99%

“…Among the existing cationic polymers, PEI is the most commonly used and is generally accepted as the gold standard for nonviral gene delivery [ 15 , 64 ]. This polymer has a high density of amine groups which, not only enhance the electrostatic interaction with genetic material and the cellular uptake but also help to avoid the endo/lysosomal pathway degradation due to the “proton sponge effect” [ 64 , 69 ]. This process is characterized by the protonation of amine groups upon acidification in the endosome, leading to increased osmotic pressure and eventual swelling and burst of the endosome, consequently releasing the nanosystem into the cytoplasm [ 79 , 80 ].…”

Section: Hybrid Silica Nanoparticlesmentioning

confidence: 99%

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Silica-Based Gene Delivery Systems: From Design to Therapeutic Applications

2020

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Advances in gene therapy have been foreshadowing its potential for the treatment of a vast range of diseases involving genetic malfunctioning. However, its therapeutic efficiency and successful outcome are highly dependent on the development of the ideal gene delivery system. On that matter, silica-based vectors have diverted some attention from viral and other types of non-viral vectors due to their increased safety, easily modifiable structure and surface, high stability, and cost-effectiveness. The versatility of silane chemistry and the combination of silica with other materials, such as polymers, lipids, or inorganic particles, has resulted in the development of carriers with great loading capacities, ability to effectively protect and bind genetic material, targeted delivery, and stimuli-responsive release of cargos. Promising results have been obtained both in vitro and in vivo using these nanosystems as multifunctional platforms in different potential therapeutic areas, such as cancer or brain therapies, sometimes combined with imaging functions. Herein, the current advances in silica-based systems designed for gene therapy are reviewed, including their main properties, fabrication methods, surface modifications, and potential therapeutic applications.

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Section: Hybrid Silica Nanoparticlesmentioning

confidence: 99%

Section: Hybrid Silica Nanoparticlesmentioning

confidence: 99%

Section: Hybrid Silica Nanoparticlesmentioning

confidence: 99%

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Silica-Based Gene Delivery Systems: From Design to Therapeutic Applications

2020

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“…Each one of these variables may be responsible for the difference in DNA transfer capability, as well as a combination of all. Molar mass is related to the size of the polyplex formed by combination with DNA, as well as toxicity (14,(35)(36)(37). Differential composition is related to the hydrophilic/hydrophobic balance, which can certainly be a major factor to the interaction of the polyplex with the bacteria membrane.…”

Section: Resultsmentioning

confidence: 99%

A novel method for DNA delivery into bacteria using cationic copolymers

Souza

Vitale

Florenzano

et al. 2021

Braz J Med Biol Res

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Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described. The reversible additionfragmentation chain transfer (RAFT) polymer synthesis technique and the reversible deactivation radical polymerization variants allow the design of polymers with well-controlled molar mass, morphology, and hydrophilicity/hydrophobicity ratios. RAFT was used to synthesize two amphiphilic copolymers containing different ratios of the amphiphilic poly [2-(dimethyl-amino) ethyl methacrylate] and the hydrophobic poly [methyl methacrylate]. These copolymers bound to pUC-19 DNA and successfully transfected non-competent Escherichia coli DH5a, with transformation efficiency in the range of 10 3 colony-forming units per mg of plasmid DNA. These results demonstrate prokaryote transformation using polymers with controlled amphiphilic/hydrophobic ratios.

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“…The former consists of lipid-based vectors, natural and synthetic polymers and peptide-based vectors [ 12 – 14 ]. There are a variety of types of nVVs that are commonly used with Lipofectamine 2000 [ 15 ] and polyethylenimine (PEI) [ 16 , 17 ] as the gold standard for sufficient transfection efficiency. Inorganic vectors mainly include calcium phosphates (CaPs) [ 18 ] and metal nanoparticles [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Non-viral gene delivery systems for tissue repair and regeneration

et al. 2018

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Critical tissue defects frequently result from trauma, burns, chronic wounds and/or surgery. The ideal treatment for such tissue loss is autografting, but donor sites are often limited. Tissue engineering (TE) is an inspiring alternative for tissue repair and regeneration (TRR). One of the current state-of-the-art methods for TRR is gene therapy. Non-viral gene delivery systems (nVGDS) have great potential for TE and have several advantages over viral delivery including lower immunogenicity and toxicity, better cell specificity, better modifiability, and higher productivity. However, there is no ideal nVGDS for TRR, hence, there is widespread research to improve their properties. This review introduces the basic principles and key aspects of commonly-used nVGDSs. We focus on recent advances in their applications, current challenges, and future directions.

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High transfection efficiency promoted by tailor-made cationic tri-block copolymer-based nanoparticles

Cited by 29 publications

References 33 publications

Silica-Based Gene Delivery Systems: From Design to Therapeutic Applications

Silica-Based Gene Delivery Systems: From Design to Therapeutic Applications

A novel method for DNA delivery into bacteria using cationic copolymers

Non-viral gene delivery systems for tissue repair and regeneration

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