High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

Abstract

Background Contemporary biotechnology focuses on many problems related to the functioning of developed societies. Many of these problems are related to health, especially with the rapidly rising numbers of people suffering from civilization diseases, such as obesity or diabetes. One factor contributing to the development of these diseases is the high consumption of sucrose. A very promising substitute for this sugar has emerged: the polyhydroxy alcohols, characterized by low caloric value and sufficient sweetness to replace table sugar in food production. Results In the current study, yeast belonging to the Yarrowia clade were tested for erythritol, mannitol and arabitol production using crude glycerol from the biodiesel and soap industries as carbon sources. Out of the 13 tested species, Yarrowia divulgata and Candida oslonensis turned out to be particularly efficient polyol producers. Both species produced large amounts of these compounds from both soap-derived glycerol (59.8–62.7 g dm−3) and biodiesel-derived glycerol (76.8–79.5 g dm−3). However, it is equally important that the protein and lipid content of the biomass (around 30% protein and 12% lipid) obtained after the processes is high enough to use this yeast in the production of animal feed. Conclusions The use of waste glycerol for the production of polyols as well as utilization of the biomass obtained after the process for the production of feed are part of the development of modern waste-free technologies.

The microbial production of kynurenic acid using Yarrowia lipolytica yeast growing on crude glycerol and soybean molasses

“…Due to this fact, in the presented work, crude glycerol obtained from the biodiesel industry was chosen as a substrate for KYNA production using Y. lipolytica as a microbial factor for its biosynthesis. It is also worth to point out that the global increasing production of biodiesel delivers annually high amount of crude glycerol ( Johnson and Taconi, 2007 ; Rakicka-Pustułka et al, 2021 ). The possibility to use this feedstock without any expensive purification steps for KYNA biosynthesis is a huge advantage of the presented process.…”

“…The protein content was determined in the dry biomass using the Kjeldahl method ( Kjeldahl, 1883 ). Furthermore, the lipid concentration in the obtained biomass was determined using the methodology described previously by Rakicka-Pustułka et al (2021) .…”

Optimized conversion of wheat straw into single cell oils by Yarrowia lipolytica and Lipomyces tetrasporus and synthesis of advanced biofuels

“…Moderate amounts of mannitol in the range 1.3-5.8 gL -1 were also detected. Some authors described the production of citric acid by Y. lipolytica strain DSM 8218 [33], erythritol, mannitol, and arabitol using different Y. lipolytica strains [34], isocitrate, using the strain VKM Y-2373 [35], alpha-ketoglutarioc acid using the strain A8 [36], and acetic acid using 6 different Y. lipolytica strains [37]. Although the production of mannitol was already observed in several Y. lipolytica strains, no specific data were reported so far for the strain DSM 8218 grown on sugars and the results presented in this paper enlarge the knowledge base of this microorganism.…”

Innovations and developments in single cell protein: Bibliometric review and patents analysis

“…Thus, a practice that has been trialled is the use of by-products from food and beverage processing industries, as well as forestry and agricultural sources, for the production of SCP as an economic and environmental strategy to reduce costs and reuse waste. (Ritala et al, 2017;Rakicka-Pustułka et al, 2021). The use of agro-industrial residues is further of interest because these sources provide the carbon and nitrogen necessary for the growth of microorganisms (Anupamaa and Ravindra, 2000).…”