Higher amplitudes in steady-state visual evoked potentials driven by square-wave versus sine-wave contrast modulation – a dual-laboratory study.

Abstract

Steady-state visual evoked potentials (ssVEPs) are an established tool for assessing visuocortical responses in visual perception and attention. They have the same temporal frequency characteristics as a periodically modulated stimulus (e.g., in contrast or luminance) that drives them. It has been hypothesized that the amplitude of a given ssVEP may depend on the shape of the stimulus modulation function, but the size and robustness of these effects is not well established. The current study systematically compared the effect of the two most common functions in the ssVEP literature, square-wave and sine-wave functions. Across two laboratories, we presented mid-complex color patterns to 30 participants with square-wave or sine-wave contrast modulation and at different driving frequencies (6 Hz, 8.57 Hz, 15 Hz). When ssVEPs were analyzed independently for the samples, with each laboratory’s standard processing pipeline, ssVEP amplitudes in both samples decreased at higher driving frequencies and square-wave modulation evoked higher amplitudes at lower frequencies (i.e., 6 Hz, 8.57 Hz) compared to sine-wave modulation. These effects were replicated when samples were aggregated and analyzed with the same processing pipeline. In addition, when using signal-to-noise ratios as outcome measures, this joint analysis indicated a somewhat weaker effect of increased ssVEP amplitudes to square-wave modulation at 15 Hz. The present study suggests that square-wave modulation should be used in ssVEP research when the goal is to maximize signal amplitude or signal-to-noise ratio. Given effects of modulation function across laboratories, and data processing pipelines, the findings appear robust to differences in data collection and analysis.