Higher anti-heparan sulphate reactivity during systemic lupus erythematosus (SLE) disease exacerbations with renal manifestations; a long term prospective analysis

Kramers, C.; Termaat, R. M.; Borg, E. J. Ter; Bruggen, M.C.J. van; Kallenberg, Cees G. M.; Berden, Jo H. M.

doi:10.1111/j.1365-2249.1993.tb06493.x

Cited by 29 publications

(5 citation statements)

References 27 publications

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“…Concerning other autoantibodies tested such as antiphospholipid antibodies, no association with LN was found thus confirming previous reports as well [21]. Other studies have found that LN patients had significantly higher levels of anti-dsDNA antibodies than patients with no renal disease [23,24]. Anti-dsDNA antibodies may be present in the sera of SLE patients much earlier than previously suspected.…”

Section: Discussionsupporting

confidence: 88%

Lupus Nephritis in Colombians: Contrasts and Comparisons with Other Populations

Anaya

Cañas

Mantilla

et al. 2011

Clinic Rev Allerg Immunol

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Lupus nephritis (LN) is one of the most serious complications of systemic lupus erythematosus (SLE) since it is the major predictor of poor prognosis. The purpose of this study was to examine the clinical and immunological characteristics associated with LN development during the course of SLE in Colombians. Therefore, patients with SLE followed at five different referral centers in Medellin, Bogota, and Cali were included in this cross-sectional and multicenter study. Factors influencing LN were assessed by conditional logistic regression analysis, adjusting by gender, age at onset, duration of disease, and city of origin. The entire sample population included 467 patients, of whom 51% presented with LN. The presence of anti-dsDNA antibodies (adjusted odds ratio (AOR), 2.06; 95% confidence interval (CI), 1.16-3.65), pleuritis (AOR, 3.82; 95% CI, 1.38-10.54), and hypertension (AOR, 2.63; 95% CI, 1.23-5.62) were positively associated with LN, whereas the presence of anti-La antibodies was a protective factor against LN development (AOR, 0.4; 95% CI, 0.19-0.85). A review of literature on LN in different populations is made. The identified clinical- and laboratory-associated factors would assist earlier diagnosis and guide decisions on therapeutic interventions on this critical and frequent complication of SLE.

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Section: Discussionsupporting

confidence: 88%

Lupus Nephritis in Colombians: Contrasts and Comparisons with Other Populations

Anaya

Cañas

Mantilla

et al. 2011

Clinic Rev Allerg Immunol

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“…41 Cross-sectional and long term prospective studies reported exclusive correlation of the anti-HS reactivity mediated by anti-DNA antibodies with exacerbation of lupus presenting nephritis. 41,42 However, these studies were limited to SLE patients according to the presence or absence of nephritis.…”

Section: Discussionmentioning

confidence: 99%

Anti-heparan sulfate antibody and functional loss of glomerular heparan sulfate proteoglycans in lupus nephritis

Kim

Hong

Kim

et al. 2016

Lupus

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Background The purpose of this study was to evaluate the features of heparan sulfate proteoglycans (HSPGs) as agrins of the glomerular basement membrane (GBM) and circulating anti-heparan sulfate (HS) antibodies in lupus nephritis, comparing titers among the following groups: lupus nephritis (LN), non-renal lupus, non-lupus nephritis, and healthy controls. Methods The stage of nephritis was determined based on the kidney biopsy. Alcian blue staining and immunohistochemical (IHC) staining for agrin were performed for histological evaluation of GBM HSPGs in normal glomeruli, non-lupus membranous glomerulonephritis (MGN), and lupus MGN. The results were used for measurement of the serum anti-HS antibody titers using an enzyme-linked immunosorbent assay (ELISA) in the following groups: 38 healthy controls, 38 non-lupus nephritis, 37 non-renal lupus, and 38 LN. Results Glomerulus HSPGs were stained bluish-green along the GBM with Alcian blue. However, IHC staining against agrin was almost completely negative in the lupus MGN group compared with the normal and non-lupus MGN groups, which showed brown staining of GBM. A higher level of anti-HS IgG was detected in LN compared with other groups, respectively. Higher titers were associated with the presence of SLE and nephritis. A higher degree of proteinuria normalized to glomerular filtration rate (eGFR) was observed in association with higher anti-HS antibody titers in LN. Conclusion This study demonstrated a functional loss of GBM HSPGs and higher levels of circulating anti-HS antibodies as a characteristic feature of lupus nephritis, suggesting their involvement in the pathogenesis of lupus nephritis and proteinuria.

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“…506 [7 9], In some instances, this is attributable to the binding of anti-DNA antibodies to DNA thai is associated with the apparently cross-reactive antigens, leading to in situ immune complex formation [10]. Such indirect reactivity of anti-DNA antibodies towards heparan sulphate has been demonstrated to correlate with exacerbations of lupus nephritis [11].…”

Section: Introductionmentioning

confidence: 99%

Endothelial cell binding by human polyclonal anti-DNA antibodies: relationship to disease activity and endothelial functional alterations

Chan

Tsang

et al. 1995

Clinical and Experimental Immunology

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SUMMARY Polyclonal anti‐dsDNA and anti‐ssDNA antibodies (PoAb) that showed significant binding to human umbilical vein endothelial cells (HUVEC) were isolated from eight patients with systemic lupus erythematosus (SLE). Anti‐dsDNA PoAbs from five patients and anti‐ssDNA PoAbs from seven patients demonstrated enhanced binding to HUVEC during active disease, compared with PoAbs obtained from corresponding patients during remission. Reduction of the DNA content in the PoAb preparations by DNase treatment was associated with enhanced binding to HUVEC in 20 of 32 PoAbs tested, which included 75%‘active disease’ PoAbs, and with reduced binding to HUVEC in three of 32 PoAbs tested, all obtained during remission. Such altered endothelial cell binding was reversed with DNA reconstitution. Binding of the remaining nine PoAbs to HUVEC was not altered by variations in their DNA content. Induced plasma membrane expression of E‐selectin, but reduced expression of vascular cell adhesion molecule‐1 (VCAM‐1) by HUVEC, was observed following incubation of HUVEC with ‘active disease’ PoAbs from three and two of the eight patients, respectively. PoAbs and serum samples from two of the eight patients during active disease induced von Willebrand factor release from HUVEC, which was not observed during remission. We conclude that anti‐DNA antibodies from selected patients with SLE can bind to endothelial cells. Correlation between cellular binding and disease activity suggests that such binding of anti‐DNA antibodies to endothelial cells could be of pathogenic significance. Preliminary data also suggest that the expression of adhesion molecules and haemostatic factor(s) by endothelial cells may be modified following their binding by anti‐DNA antibodies.

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Higher anti-heparan sulphate reactivity during systemic lupus erythematosus (SLE) disease exacerbations with renal manifestations; a long term prospective analysis

Cited by 29 publications

References 27 publications

Lupus Nephritis in Colombians: Contrasts and Comparisons with Other Populations

Lupus Nephritis in Colombians: Contrasts and Comparisons with Other Populations

Anti-heparan sulfate antibody and functional loss of glomerular heparan sulfate proteoglycans in lupus nephritis

Endothelial cell binding by human polyclonal anti-DNA antibodies: relationship to disease activity and endothelial functional alterations

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