Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen

Hashimoto, Tomoko; Kojima, Katsura; Tamada, Yasushi

doi:10.3390/molecules25081939

Cited by 23 publications

(15 citation statements)

References 28 publications

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“…On day 14, we found ECM-related GO terms, such as “extracellular matrix organization” and “collagen catabolic process,” and GO terms related to cell migration and proliferation, such as “positive regulation of cell migration” and “positive regulation of cell proliferation” ( Figure 2 C, Table S3 ). Thus, gene expression changes in collagen discs are similar to those occurring in scar tissues in vivo [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model

et al. 2022

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Scars are composed of stiff collagen fibers, which contract strongly owing to the action of myofibroblasts. To explore the substances that modulate scar contracture, the fibroblast-populated collagen lattice (FPCL) model has been used. However, the molecular signature of the patient-derived FPCL model has not been verified. Here, we examined whether the patient-derived keloid FPCL model reflects scar contraction, analyzing detailed gene expression changes using comprehensive RNA sequencing and histological morphology, and revealed that these models are consistent with the changes during human scar contracture. Moreover, we examined whether conditioned media derived from adipose stem cells (ASC-CM) suppress the scar contracture of the collagen disc. Detailed time-series measurements of changes in disc area showed that the addition of ASC-CM significantly inhibited the shrinkage of collagen discs. In addition, a deep sequencing data analysis revealed that ASC-CM suppressed inflammation-related gene expression in the early phase of contraction; in the later phase, this suppression was gradually replaced by extracellular matrix (ECM)-related gene expression. These lines of data suggested the effectiveness of ASC-CM in suppressing scar contractures. Therefore, the molecular analysis of the ASC-CM actions found in this study will contribute to solving medical problems regarding pathological scarring in wound prognosis.

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Section: Resultsmentioning

confidence: 99%

Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model

et al. 2022

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“…The main stages of cell growth on the biomaterial surface include cell adhesion, proliferation, differentiation and ECM formation, and these stages are regulated by different tissue repair-related genes ( Hashimoto et al, 2020 ). COL-I is the main structural component of dermal tissue, and is higher in the dermis of normal skin, and COL-1 content can affects skin structure, function, and texture characteristics.…”

Section: Resultsmentioning

confidence: 99%

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Hou

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Skin wound healing is an important clinical challenge, and the main treatment points are accelerating epidermal regeneration and preventing infection. Therefore, it is necessary to develop a wound dressing that can simultaneously cure bacterial infections and accelerate wound healing. Here, we report a multifunctional composite wound dressing loaded with chitosan (CS)-binding bFGF (CSBD-bFGF) and antimicrobial peptides (P5S9K). First, CS was used as the dressing matrix material, and P5S9K was encapsulated in CS. Then, CSBD-bFGF was designed by combining recombinant DNA technology and tyrosinase treatment and modified on the dressing material surface. The results show that the binding ability of CSBD-bFGF and CS was significantly improved compared with that of commercial bFGF, and CSBD-bFGF could be controllably released from the CS dressing. More importantly, the prepared dressing material showed excellent antibacterial activity in vivo and in vitro and could effectively inhibit the growth of E. coli and S. aureus. Using NIH3T3 cells as cellular models, the results showed that the CSBD-bFGF@CS/P5S9K composite dressing was a friendly material for cell growth. After cells were seeded on the composite dressing surface, collagen-1 (COL-1) and vascular endothelial growth factor (VEGF) genes expression in cells were significantly upregulated. Finally, the full-thickness wound of the rat dorsal model was applied to analyse the tissue repair ability of the composite dressing. The results showed that the composite dressing containing CSBD-bFGF and P5S9K had the strongest ability to repair skin wounds. Therefore, the CSBD-bFGF@CS/P5S9K composite dressing has good antibacterial and accelerated wound healing abilities and has good application prospects in the treatment of skin wounds.

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“…In this context, Scheibel et al established a silk protein based on the consensus sequence of A. diadematus Fibroin 4 (eADF4(C16); MW 48 kDa) (Huemmerich et al 2004, Petzold et al 2017, Saric and Scheibel 2019, Salehi et al 2020. In previous studies, we and others, have demonstrated that silk-based scaffolds are biocompatible without provoking immunological reaction (Zeplin et al 2014, Ju et al 2016, Zhang et al 2017, Steiner et al 2019, Hashimoto et al 2020, Kramer et al 2020. In the present study we were able to show macrophages at the interface between the newly formed tissue and eADF4(C16) hydrogels.…”

Section: Discussionmentioning

confidence: 99%

Enhanced vascularization and de novo tissue formation in hydrogels made of engineered RGD-tagged spider silk proteins in the arteriovenous loop model

et al. 2021

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Due to its low immunogenic potential and the possibility to fine-tune their properties, materials made of recombinant engineered spider silks are promising candidates for tissue engineering applications. However, vascularization of silk-based scaffolds is one critical step for the generation of bioartificial tissues and consequently for clinical application. To circumvent insufficient vascularization, the surgically induced angiogenesis by means of arteriovenous loops (AVL) represents a highly effective methodology. Here, previously established hydrogels consisting of nano-fibrillary recombinant eADF4(C16) were transferred into Teflon isolation chambers and vascularized in the rat AVL model over 4 weeks. To improve vascularization, also RGD-tagged eADF4(C16) hydrogels were implanted in the AVL model over 2 and 4 weeks. Thereafter, the specimen were explanted and analyzed using histology and microcomputed tomography. We were able to confirm biocompatibility and tissue formation over time. Functionalizing eADF4(C16) with RGD-motifs improved hydrogel stability and enhanced vascularization even outperforming other hydrogels, such as fibrin. This study demonstrates that the scaffold ultrastructure as well as biofunctionalization with RGD-motifs are powerful tools to optimize silk-based biomaterials for tissue engineering applications.

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Higher Gene Expression Related to Wound Healing by Fibroblasts on Silk Fibroin Biomaterial than on Collagen

Cited by 23 publications

References 28 publications

Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model

Therapeutic Potential of Adipose Stem Cell-Derived Conditioned Medium on Scar Contraction Model

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Enhanced vascularization and de novo tissue formation in hydrogels made of engineered RGD-tagged spider silk proteins in the arteriovenous loop model

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