Higher Hypochlorous Acid Scavenging Activity of Ethyl Pyruvate Compared to Its Sodium Salt

Olek, Robert A.; Ziółkowski, Wiesław; Kaczor, Jan Jacek; Wierzba, Tomasz H.; Antosiewicz, Jędrzej

doi:10.1271/bbb.100728

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…Moreover, reactive oxygen species production has been considered as one of several adverse cellular responses to nutrient excess in obesity (33). Since EtP has been demonstrated to be an effective antioxidant (34,35), the markers of oxidative stress have been determined. Decreased CAT activity as a consequence of HFD was noted, similarly to previous studies (33,36).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ethyl Pyruvate Supplementation on Rat Fatty Liver Induced by a High-Fat Diet

Olek

Ziółkowski

Flis

et al. 2013

J Nutr Sci Vitaminol

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Continuous positive energy imbalance leads to obesity, which increases the risk of developing non-alcoholic fatty liver disease. The hepatoprotective effect of ethyl pyruvate has been revealed in several studies. Therefore, we examined the effect of ethyl pyruvate supplementation on liver cell damage, metabolism, membrane fluidity, and oxidative stress markers in rats fed a high-fat diet. After 6-wk feeding of a control or high-fat diet, Wistar rats were divided into 4 groups: control diet, control diet and ethyl pyruvate, high-fat diet, and high-fat diet and ethyl pyruvate. Ethyl pyruvate was administered as a 0.3% solution in drinking water, for the following 6 wk. Ethyl pyruvate intake attenuated the increase in activities of plasma transaminases and liver TNF-α. However, the supplementation was without effect in the lipid profiles, membrane fluidity or oxidative metabolism in liver induced by the high-fat diet. Our data confirm the potency of ethyl pyruvate against cell liver damage. Nevertheless, prolonged intake did not affect the development of a fatty liver.

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Section: Discussionmentioning

confidence: 99%

The Effect of Ethyl Pyruvate Supplementation on Rat Fatty Liver Induced by a High-Fat Diet

Olek

Ziółkowski

Flis

et al. 2013

J Nutr Sci Vitaminol

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“…Moreover, the forms of pyruvate used as a supplement may play a pivotal role. It has been shown that properties varies between pyruvate esters and salts with the higher effectiveness of EtP than its salt [22,23,24,25,26,27]. Furthermore, anti-inflammatory properties of EtP have been related to a reduction of c-Jun NH 2 -terminal kinase phosphorylation [45,46], which is an important signaling protein involved in the skeletal muscle insulin resistance pathway [47].…”

Section: Discussionmentioning

confidence: 99%

“…By a nonenzymatic reaction it reduces hydrogen peroxide to water [20] and scavenges hydroxyl radical [21]. The effectiveness of ethyl pyruvate (EtP) has been proven in various stress conditions [22,23,24,25,26,27]. …”

Section: Introductionmentioning

confidence: 99%

Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

et al. 2013

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Impaired mitochondrial capacity may be implicated in the pathology of chronic metabolic diseases. To elucidate the effect of ethyl pyruvate supplementation on skeletal muscles metabolism we examined changes in activities of mitochondrial and antioxidant enzymes, as well as sulfhydryl groups oxidation (an indirect marker of oxidative stress) during the development of obesity. After 6 weeks feeding of control or high fat diet, Wistar rats were divided into four groups: control diet, control diet and ethyl pyruvate, high fat diet, and high fat diet and ethyl pyruvate. Ethyl pyruvate was administered as 0.3% solution in drinking water, for the following 6 weeks. High fat diet feeding induced the increase of activities 3-hydroxyacylCoA dehydrogenase, citrate synthase, and fumarase. Moreover, higher catalase and superoxide dismutase activities, as well as sulfhydryl groups oxidation, were noted. Ethyl pyruvate supplementation did not affect the mitochondrial enzymes’ activities, but induced superoxide dismutase activity and sulfhydryl groups oxidation. All of the changes were observed in soleus muscle, but not in extensor digitorum longus muscle. Additionally, positive correlations between fasting blood insulin concentration and activities of catalase (p = 0.04), and superoxide dismutase (p = 0.01) in soleus muscle were noticed. Prolonged ethyl pyruvate consumption elevated insulin concentration, which may cause modifications in oxidative type skeletal muscles.

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“…The reactions of pyruvate ion have extensively been studied in recent years due to the specic role of this species in various biological redox processes. [17][18][19][20] Furthermore, the simplicity of the molecule allows modelling the oxidation of organic substrates in the presence of various oxidants. [21][22][23][24][25][26] Pyruvic acid is a simple substrate with a carbonyl group next to a carboxylic group.…”

Section: Introductionmentioning

confidence: 99%

The kinetics and mechanism of the oxidation of pyruvate ion by hypochlorous acid

et al. 2015

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The oxidation of pyruvic acid by hypochlorous acid was studied in the pH 1.0 -11.0 range. The main path in the redox process is the reaction between the pyruvate ion and HOCl. It was shown that the reaction is second order in both reactants and kHOCl = 2.14 ± 0.02M −1 s −1 (I = 1.0 M (NaClO4), T = 25.0 °C) with ∆HHOCl ≠ = 34.6 ± 1.2 kJmol −1 and ∆SHOCl ≠ = −120.3 ± 3.6 Jmol −1 K −1 for this reaction step. The relatively large negative entropy of activation is consistent with an O atom-transfer mechanism. DFT calculations support this conclusion by predicting a concerted rearrangement of the activated complex which includes the reactants and the solvent water molecule. It was also confirmed that the hydration of pyruvic acid has significant contribution to the observed kinetic features under acidic condition. The hydration reaction is a proton catalyzed process and the pseudo-first order rate constant can be interpreted by considering theprotolytic and hydration equilibria of pyruvic acid. The rate constants were determined for the non-catalytic and the proton catalyzed path: kh0 = 0.24 ± 0.01 s −1 and kh1 = 5.5 ± 0.2 M −1 s −1 .

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Higher Hypochlorous Acid Scavenging Activity of Ethyl Pyruvate Compared to Its Sodium Salt

Cited by 5 publications

References 47 publications

The Effect of Ethyl Pyruvate Supplementation on Rat Fatty Liver Induced by a High-Fat Diet

The Effect of Ethyl Pyruvate Supplementation on Rat Fatty Liver Induced by a High-Fat Diet

Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

The kinetics and mechanism of the oxidation of pyruvate ion by hypochlorous acid

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