Highlighting anatomical sub-structures in rat brain tissue using lipid imaging

Delvolvé, Alice; Colsch, Benoît; Woods, Amina S.

doi:10.1039/c1ay05107e

Cited by 22 publications

(31 citation statements)

References 54 publications

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“…Delvolve et al also revealed SM (d18:1/18:0) to be distributed in the GM, and SM (d18:1/24:1) to be distributed in the WM, in rat brain sections using MALDI-LTQ-XL [38]. Even though we used a different method and animal species, our results were in good agreement with their data.…”

Section: Maldi-fticr-imssupporting

confidence: 82%

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Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains

Sugimoto

Shimizu

Yoshioka

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Sphingomyelin (SM) is synthesized by SM synthase (SMS) from ceramide (Cer). SM regulates signaling pathways and maintains organ structure. SM comprises a sphingoid base and differing lengths of acyl-chains, but the importance of its various forms and regulatory synthases is not known. It has been reported that Cer synthase (CerS) has restricted substrate specificity, whereas SMS has no specificity for different lengths of acyl-chains.We hypothesized that the distribution of each SM molecular species was regulated by expression of the CerS family. Thus, we compared the distribution of SM species and CerS mRNA expression using molecular imaging. Spatial distribution of each SM molecular species was investigated using ultra-high-resolution imaging mass spectrometry (IMS).IMS revealed that distribution of SM molecular species varied according to the lengths of acyl-chains found in each brain section. Furthermore, a combination study using in situ hybridization and IMS revealed the spatial expression of CerS1 to be associated with the localization of SM (d18:1/18:0) in cell body-rich gray matter, and CerS2 to be associated with SM (d18:1/24:1) in myelin-rich white matter. Our study is the first comparison of spatial distribution between SM molecular species and CerS isoforms, and revealed their distinct association in the brain. These observations were demonstrated by suppression of CerS2 using siRNA in HepG2 cells; that is, siRNA for CerS2 specifically decreased C22 very long-chain fatty acid (VLCFA)-and C24 VLCFA-containing SMs. Thus, histological analyses of SM species by IMS could be a useful approach to consider their molecular function and regulative mechanism.

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Section: Maldi-fticr-imssupporting

confidence: 82%

“…[29][30][31][32][33]. Several reports have shown that MALDI-IMS can be used for imaging of the sites of endogenous metabolites, especially glycerophospholipids and sphingolipids [34][35][36][37][38]. Furthermore, use of MS/MS allows the structures of imaged molecules to be identified [39,40].…”

Section: Introductionmentioning

confidence: 99%

Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains

Sugimoto

Shimizu

Yoshioka

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…One productive approach has been to use matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) imaging in rat brain tissue sections to identify lipid species (Jackson et al, 2005a, 2005b). Subsequent studies have described discrete localization of lipids throughout both the rat (Delvolve et al, 2011; Mikawa et al, 2009) and the human brain (Veloso et al, 2011a, 2011b). These observations suggest that heterogeneity in the relative abundance of lipid species both within and between brain regions may have functional relevance.…”

Section: Introductionmentioning

confidence: 99%

Differential effects of cocaine exposure on the abundance of phospholipid species in rat brain and blood

Cummings

Pati

Şahin

et al. 2015

Drug and Alcohol Dependence

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Background Lipid profiles in the blood are altered in human cocaine users, suggesting that cocaine-exposure can induce lipid remodeling. Methods Cocaine-induced locomotor sensitization in rats was followed by shotgun lipidomics using electrospray ionization-mass spectrometry (ESI-MS) and determined changes in brain tissues. To determine if any lipidomic changes were also reflected in the blood, we performed principal component analysis (PCA) of lipidomic spectra isolated from cocaine-treated animals. Alterations in the abundance of phospholipid species were correlated with behavioral changes in the magnitude of either the initial response to drug or locomotor sensitization. Results Behavioral sensitization altered the relative abundance of several phospholipid species in the hippocampus and cerebellum, measured one week following the final exposure to cocaine. In contrast, relatively few effects on phospholipids in either the dorsal or the ventral striatum were observed. PCA analysis demonstrated that cocaine altered the relative abundance of several glycerophospholipid species as compared to saline-injected controls. Subsequent MS/MS analysis identified some of these lipids as phosphatidylethanolamines, phosphatidylserines and phosphatidylcholines. The relative abundance of some of these phospholipid species were well correlated (R2 of 0.7 or higher) with either the initial response to cocaine or locomotor sensitization. Conclusion Taken together, these data demonstrate that a cocaine-conditioning experience results in the remodeling of specific phospholipids in rat brain tissue in a region-specific manner and also alters the intensities and types of phospholipid species in rat blood. These results further suggest that such changes may serve as biomarkers to assess the neuroadaptations occurring following repeated exposure to cocaine.

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“…The unique advantages of IMS include the following: (i) simultaneous detection of multiple lipids in the same specimen, which has shown that slight structural differences in lipid molecules produce a heterogeneous distribution pattern in biological tissues [13,14,[18][19][20]; (ii) application to a wide range of analytes [21], ranging from small and simple to large and more complex lipids such as glycolipids with complex oligosaccharide moieties [22,23]; (iii) a simple and fast protocol [17]. In fact, using SCI model rats, a recent study by Cooks' group found that specific lipid species accumulated in the lesion epicenter and adjacent areas after SCI [13].…”

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confidence: 99%

Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury

et al. 2012

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We determined quantitative and qualitative alterations in lipids during the occurrence and progression of spinal cord injury (SCI) in rats to identify potential clinical indicators of SCI pathology. Imaging mass spectrometry (IMS) was used to visualize twelve molecular species of phosphatidylcholine (PC) on thin slices of spinal cord with SCI. In addition, twelve species of phospholipids and five species of prostaglandins (PGs) were quantified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) of lipid extracts from control/injured spinal cords. Unique distribution patterns were observed for phospholipids with different fatty acid compositions, and distinct dynamic changes were seen in both their amounts and their distributions in tissue as tissue damage resulting from SCI progressed. In particular, PCs containing docosahexaenoic acid localized to the large nucleus in the anterior horn region at one day post-SCI and rapidly decreased thereafter. In contrast, PCs containing arachidonic acid (AA-PCs) were normally found in the posterior horn region and were intensely and temporarily elevated one week after SCI. Lysophosphatidylcholines (LPCs) also increased at the same SCI stage and in regions with elevated AA-PCs, indicating the release of AA and the production of PGs. Moreover, LC-ESI-MS/MS analysis of lipid extracts from the spinal cord tissue at the impact site demonstrated a peak in PGE2 that reflected the elevation/reduction pattern of AA-PCs and LPC. Although further investigation is required, we suggest that invasive immune cells that penetrated from the impaired blood-brain barrier at 1-2 weeks post-SCI may have produced LPCs, released AA from AA-PCs, and produced PGs in SCI tissue at sites enriched in AA-PCs/LPC.

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Highlighting anatomical sub-structures in rat brain tissue using lipid imaging

Cited by 22 publications

References 54 publications

Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains

Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains

Differential effects of cocaine exposure on the abundance of phospholipid species in rat brain and blood

Spatiotemporal alteration of phospholipids and prostaglandins in a rat model of spinal cord injury

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