Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA

Frohnmeyer, Esther; Frisch, Farina; Falke, Sven; Betzel, Christian; Fischer, Markus

doi:10.1016/j.jbiotec.2018.01.012

Cited by 34 publications

(16 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many efforts have been made to enhance the sensitivity and selectivity of biosensors based on aptamers [101], however, little attention have been focused on the improvement of the response rate of biosensor. The sensible engineering of a quickly responsive and highly sensitive aptamer-cDNA probe was realized by utilizing bivalent interaction of two short monovalent cDNA sequences, then the probe was hybridized simutaneously to two electrode-imobilized aptamer-based probes combined with eletrochemical aptamers, which could enhance the response rate from 30 min to 4 min [102,103]. The latest developed aptasensors are summarized in Figure 3.…”

Section: Recent Advances In Detecting Toxins By Aptasensorsmentioning

confidence: 99%

“…Although aptasensors have been widely used in different fields [102][103][104][105][106][107][108], great efforts still need to be made in the following respects: (1) the optimization of aptamer selection process. Although some aptamers for marine toxins have been selected, it is still not sufficient for a large amount of marine toxins with categories of more than 1000.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

View full text Add to dashboard Cite

Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.Key Contribution: This article reviews systematically the toxicity of marine toxins, the development of aptarmer-based biosensors, and progress in the marine toxin detection by aptasensors.Toxins 2020, 12, 1 2 of 22 selection technology and biosensor fabrication technology offer a new solution for the detection of marine toxins with high efficiency and sensitivity [13][14][15][16][17].Marine toxins are a class of small molecular compounds mainly produced by red tide algae. In recent years, with the aggravation of environmental pollution, human deaths due to accidental ingestion of toxic shellfish are also often reported [18][19][20][21]. At present, the main methods for the detection of marine toxins include mouse bioassay (MBA) [22], enzyme-linked immunosorbent assay (ELISA) [23][24][25], high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), etc. [26][27][28]. However, these traditional methods have some drawbacks including the requirement of a technician, poor repeatability, expensive equipment, and issues related to animal ethics [22]. As a new detection technology, the biosensor is a kind of analysis systems using cell molecules and other bio-materials as sensitive elements, combined with a secondary sensor to detect a variety of chemicals by cascade amplified signal [29]. Because of their advantages of simple operation, high speed, high sensitivity, miniaturization, and easy automation, biosensors have been widely used in different fields including cell physiology [30], drug screening [31,32], food safety detection [33], disease diagnosis [34], etc.Aptamer, meaning "to fit", is a kind of oligonucleotide which can bind to target molecules with high selectivity and affinity. On account of the existence of aptamers in nature and the po...

show abstract

Section: Recent Advances In Detecting Toxins By Aptasensorsmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

View full text Add to dashboard Cite

show abstract

“…OSs were developed with aptamers having Kd of 0.36 µmol/L, 50-85 nmol/L and 41 nmol/L for OTA, AFs, and ZON, respectively. As low Kd values in the nmol/L range were already described for other toxins, such as ricin [192], MCs [193], FUM B1 [194], cholera toxin [195] or GTX 1 and 4 [196], and OA [197], one can expect the development of new OSs for toxins in the near future.…”

Section: Oligosorbentsmentioning

confidence: 82%

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Delaunay

Combès

Pichon

2020

Toxins

View full text Add to dashboard Cite

The evolution of instrumentation in terms of separation and detection allowed a real improvement of the sensitivity and analysis time. However, the analysis of ultra-traces of toxins in complex samples requires often a step of purification and even preconcentration before their chromatographic analysis. Therefore, immunoaffinity sorbents based on specific antibodies thus providing a molecular recognition mechanism appear as powerful tools for the selective extraction of a target molecule and its structural analogs to obtain more reliable and sensitive quantitative analysis in environmental, food or biological matrices. This review focuses on immunosorbents that have proven their efficiency in selectively extracting various types of toxins of various sizes (from small mycotoxins to large proteins) and physicochemical properties. Immunosorbents are now commercially available, and their use has been validated for numerous applications. The wide variety of samples to be analyzed, as well as extraction conditions and their impact on extraction yields, is discussed. In addition, their potential for purification and thus suppression of matrix effects, responsible for quantification problems especially in mass spectrometry, is presented. Due to their similar properties, molecularly imprinted polymers and aptamer-based sorbents that appear to be an interesting alternative to antibodies are also briefly addressed by comparing their potential with that of immunosorbents.

show abstract

“…Moreover, magnetic beads can offer additional features of capturing analytes/molecules [18] or isolating targets [19] by bio-functionalizing the magnetic beads’ surface. For example, antigen-coatedmagnetic beads have been employed as part of an ultrasensitive platform for the detection of biomolecules and proteins [20], whereas magnetic beads have been functionalized to selectively capture aptamers against cholera toxin [21]. A 2.5-fold improvement in biomarker capture for medical diagnostics was noted [22] while the formation of magnetic bead chains results in an almost 2-fold signal enhancement over the measured concentration range of a biosensor [23].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Magnetic Mixing at Low Reynolds Numbers and in Stagnant Fluids

et al. 2019

View full text Add to dashboard Cite

Microfluidic mixing becomes a necessity when thorough sample homogenization is required in small volumes of fluid, such as in lab-on-a-chip devices. For example, efficient mixing is extraordinarily challenging in capillary-filling microfluidic devices and in microchambers with stagnant fluids. To address this issue, specifically designed geometrical features can enhance the effect of diffusion and provide efficient mixing by inducing chaotic fluid flow. This scheme is known as “passive” mixing. In addition, when rapid and global mixing is essential, “active” mixing can be applied by exploiting an external source. In particular, magnetic mixing (where a magnetic field acts to stimulate mixing) shows great potential for high mixing efficiency. This method generally involves magnetic beads and external (or integrated) magnets for the creation of chaotic motion in the device. However, there is still plenty of room for exploiting the potential of magnetic beads for mixing applications. Therefore, this review article focuses on the advantages of magnetic bead mixing along with recommendations on improving mixing in low Reynolds number flows (Re ≤ 1) and in stagnant fluids.

show abstract

Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA

Cited by 34 publications

References 38 publications

Marine Toxins Detection by Biosensors Based on Aptamers

Marine Toxins Detection by Biosensors Based on Aptamers

Immunoaffinity Extraction and Alternative Approaches for the Analysis of Toxins in Environmental, Food or Biological Matrices

Microfluidic Magnetic Mixing at Low Reynolds Numbers and in Stagnant Fluids

Contact Info

Product

Resources

About