Highly Diluted Acetylcholine Promotes Wound Repair in an <i>In Vivo</i> Model

Uberti, Francesca; Morsanuto, Vera; Ghirlanda, Sabrina; Ruga, Sara; Clemente, Nausicaa; Boieri, Cristina; Boldorini, Renzo; Molinari, Claudio

doi:10.1089/wound.2017.0766

Cited by 20 publications

(20 citation statements)

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“…Furthermore, donepezil may act differently on bone and skin cells [ 35 , 36 ]. Even though donepezil does not have a local direct effect on osteoprogenitor mesenchymal stem cell (MSCs) [ 37 ], acetylcholine could accelerate keratinocyte proliferation, migration, and viability [ 38 ]. This is mediated through the action of non-neuronal cholinergic receptors; muscarinic (M1 and M3) and a7 nicotinic receptors of keratinocytes [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Even though donepezil does not have a local direct effect on osteoprogenitor mesenchymal stem cell (MSCs) [ 37 ], acetylcholine could accelerate keratinocyte proliferation, migration, and viability [ 38 ]. This is mediated through the action of non-neuronal cholinergic receptors; muscarinic (M1 and M3) and a7 nicotinic receptors of keratinocytes [ 38 , 39 ]. Moreover, donepezil could further enhance skin wound healing through a feed-back loop, in which keratinocyte activation stimulates fibroblasts to release growth factors, which in turn stimulate keratinocyte proliferation [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

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Postoperative Administration of the Acetylcholinesterase Inhibitor, Donepezil, Interferes with Bone Healing and Implant Osseointegration in a Rat Model

et al. 2020

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Donepezil is an acetylcholinesterase inhibitor commonly used to treat mild to moderate Alzheimer’s disease. Its use has been associated with increased bone mass in humans and animals. However, the effect of postoperative administration of donepezil on bone healing remains unknown. Therefore, this study aimed to assess the impact of postoperative injection of donepezil on bone healing, titanium-implant osseointegration, and soft tissue healing. Twenty-two Sprague-Dawley rats were randomly assigned to receive a daily dose of either donepezil (0.6 mg/kg) or saline as a control. In each rat, a uni-cortical defect was created in the right tibia metaphysis and a custom-made titanium implant was placed in the left tibiae. After two weeks, rats were euthanized, and their bones were analysed by Micro-CT and histology. The healing of bone defect and implant osseointegration in the rats treated with donepezil were significantly reduced compared to the saline-treated rats. Histomorphometric analysis showed lower immune cell infiltration in bone defects treated with donepezil compared to the saline-treated defects. On the other hand, the healing time of soft tissue wounds was significantly shorter in donepezil-treated rats compared to the controls. In conclusion, short-term administration of donepezil hinders bone healing whereas enhancing soft tissue healing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Postoperative Administration of the Acetylcholinesterase Inhibitor, Donepezil, Interferes with Bone Healing and Implant Osseointegration in a Rat Model

et al. 2020

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“…2.9. MMP-9 Quantification Assay MMP-9 ELISA Kit (DBA, Italy, CN KA0398) was used to quantify MMP-9 on culture supernatant samples, following the manufacturer's instructions [56]. Briefly, samples in 96-well/plate were incubated at 37 • C for 90 min, and then biotinylated antibody and ABC working solution were added and the plate was incubated at 37 • C for 60 and 30 min, respectively.…”

Section: P53 Activitymentioning

confidence: 99%

Effects of a New Combination of Natural Extracts on Glaucoma-Related Retinal Degeneration

Molinari

Ruga

Farghali

et al. 2021

Foods

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Background: Glaucoma is currently the leading cause of irreversible blindness; it is a neuropathy characterized by structural alterations of the optic nerve, leading to visual impairments. The aim of this work is to develop a new oral formulation able to counteract the early changes connected to glaucomatous degeneration. The composition is based on gastrodin and vitamin D3 combined with vitamin C, blackcurrant, and lycopene. Methods: Cells and tissues of the retina were used to study biological mechanisms involved in glaucoma, to slow down the progression of the disease. Experiments mimicking the conditions of glaucoma were carried out to examine the etiology of retinal degeneration. Results: Our results show a significant ability to restore glaucoma-induced damage, by counteracting ROS production and promoting cell survival by inhibiting apoptosis. These effects were confirmed by the intracellular mechanism that was activated following administration of the compound, either before or after the glaucoma induction. In particular, the main results were obtained as a preventive action of glaucoma, showing a beneficial action on all selected markers, both on cells and on eyecup preparations. It is therefore possible to hypothesize both the preventive and therapeutic use of this formulation, in the presence of risk factors, and due to its ability to inhibit the apoptotic cycle and to stimulate cell survival mechanisms, respectively. Conclusion: This formulation has exhibited an active role in the prevention or restoration of glaucoma damage for the first time.

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“…In this paper, the developed method was used for the study of water protein solutions (interferon-gamma (IFNγ), antibodies to interferon-gamma (Ab to IFNγ)) and glycine buffer (GB) at low concentration. The issue of sensitivity when dealing with low concentrations is of utmost importance, in particular, given the fact that some studies [2][3][4][5][6][7] have revealed the effects observed in protein solutions at low concentrations that can hardly be explained directly by the properties of protein and the solvent, i.e., water. Furthermore, the present study analyzed the properties of the protein (IFNγ) dissolved in the above-mentioned solutions at low concentration.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Emission Infrared Spectra of Protein Solutions in Low Concentrations

Penkov

Pen’kova

2020

Front. Phys.

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In this work, based on the method of infrared emission spectroscopy, the study of emission spectra of interferon-gamma (IFNγ) solution in a mixture or surrounded by three low-concentration solutions (IFNγ, antibodies to IFNγ, glycine buffer) or water control was performed. First of all, the solutions of low concentrations themselves were studied. It was shown that low-concentration solutions of IFNγ and antibodies to IFNγ had lower emission intensity in three spectral bands near 800, 1,300 and 2000 cm−1 compared to water control. Glycine buffer solution had a radiation level indistinguishable from that of the control. In this work, the effect of adding these low-concentration solutions to IFNγ (1 mg/ml) was compared to the effect of adding water control to IFNγ. All solutions or water were added in 10% (v/v). It was found that adding each of the three test solutions induced an increase in the radiation intensity of the IFNγ solution in the spectral range of 400–1700 cm−1 (compared to the IFNγ solution with control spike). It was also tested whether the radiation of the studied low-concentration solutions surrounding the IFNγ solution (1 mg/ml) affected the IFNγ radiation. The measurement results were compared to the data obtained for IFNγ surrounded by water control. All three solutions were found to exert a distant effect on the IFNγ solution (1 mg/ml), which was manifested in a decrease in the intensity of its radiation near 1,000 and 1,500 cm−1 compared to the control solution of IFNγ. Thus, the emission spectra of low-concentration aqueous solutions were measured for the first time, and differences in the emission spectra of the IFNγ solution depending on low-concentration additives and the environment were shown. The paper interprets the observed differences and discusses possible mechanisms underlying the observed phenomena.

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Highly Diluted Acetylcholine Promotes Wound Repair in an In Vivo Model

Cited by 20 publications

References 39 publications

Postoperative Administration of the Acetylcholinesterase Inhibitor, Donepezil, Interferes with Bone Healing and Implant Osseointegration in a Rat Model

Postoperative Administration of the Acetylcholinesterase Inhibitor, Donepezil, Interferes with Bone Healing and Implant Osseointegration in a Rat Model

Effects of a New Combination of Natural Extracts on Glaucoma-Related Retinal Degeneration

Analysis of Emission Infrared Spectra of Protein Solutions in Low Concentrations

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