Highly Efficient Capture and Electrochemical Release of Circulating Tumor Cells by Using Aptamers Modified Gold Nanowire Arrays

Zhai, Tingting; Ye, Dekai; Zhang, Qianwen; Wu, Zeng-Qiang; Xia, Xing‐Hua

doi:10.1021/acsami.7b11107

Cited by 70 publications

(55 citation statements)

References 61 publications

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“…For example, comparable Au NWs arrays fabricated by template-assisted electrodeposition have been described as highly efficient platforms for the capture and electrochemical release of circulating human leukemic lymphoblasts (tumor cells) when functionalized with aptamers. [53] By using electrochemical deposition on anodic aluminum oxide as a template, the arrays fabricated were able to capture target cells with much higher yield and to release them with little damage through an electrochemical desorption process. Silicon NWs (150 nm in diameter and 2.6 µm in length) decorated with Au are being also explored as highly efficient near-infrared (NIR) hyperthermia and photothermal agents for cancer treatment.…”

Section: Nanostructured Electrodes Enable Electrical Stimulation Of Bmentioning

confidence: 99%

Interfacing Neurons with Nanostructured Electrodes Modulates Synaptic Circuit Features

et al. 2020

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Understanding neural physiopathology requires advances in nanotechnology‐based interfaces, engineered to monitor the functional state of mammalian nervous cells. Such interfaces typically contain nanometer‐size features for stimulation and recording as in cell‐non‐invasive extracellular microelectrode arrays. In such devices, it turns crucial to understand specific interactions of neural cells with physicochemical features of electrodes, which could be designed to optimize performance. Herein, versatile flexible nanostructured electrodes covered by arrays of metallic nanowires are fabricated and used to investigate the role of chemical composition and nanotopography on rat brain cells in vitro. By using Au and Ni as exemplary materials, nanostructure and chemical composition are demonstrated to play major roles in the interaction of neural cells with electrodes. Nanostructured devices are interfaced to rat embryonic cortical cells and postnatal hippocampal neurons forming synaptic circuits. It is shown that Au‐based electrodes behave similarly to controls. Contrarily, Ni‐based nanostructured electrodes increase cell survival, boost neuronal differentiation, and reduce glial cells with respect to flat counterparts. Nonetheless, Au‐based electrodes perform superiorly compared to Ni‐based ones. Under electrical stimulation, Au‐based nanostructured substrates evoke intracellular calcium dynamics compatible with neural networks activation. These studies highlight the opportunity for these electrodes to excite a silent neural network by direct neuronal membranes depolarization.

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Section: Nanostructured Electrodes Enable Electrical Stimulation Of Bmentioning

confidence: 99%

Interfacing Neurons with Nanostructured Electrodes Modulates Synaptic Circuit Features

et al. 2020

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“…Compared with thiol‐containing linkers, thiol‐terminal aptamers exhibit more merits for the combined recognition capability of aptamers with convenient modification and desorption of thiol to Au substrate. Xia and co‐workers constructed cell capture‐and‐release platform based on aptamer modified gold nanowire arrays (AuNWs) . With a potential of −1.2 V to treat AuNWs for 30 s, 96.2% of the captured tumor cells were released with excellent cell viability of ≈90% ( Figure a).…”

Section: Ctc Releasementioning

confidence: 99%

“…a) Gold nanowire array platform modified with thiol‐terminal aptamers for CTC release via electrochemical breakage of AuS bond. Reproduced with permission . Copyright 2017, American Chemical Society.…”

Section: Ctc Releasementioning

confidence: 99%

“…Xia and co-workers constructed cell capture-and-release platform based on aptamer modified gold nanowire arrays (AuNWs). [127] With a potential of −1.2 V to treat AuNWs for 30 s, 96.2% of the captured tumor cells were released with excellent cell viability of ≈90% (Figure 7a). Conducting polymers, such as polypyrrole (Ppy), poly(3,4-ethylenedioxythiophene) (PEDOT)], can be alternative conductive substrates for electrochemical cell release due to their predominant electrical transport and electrochemical charge-discharge properties, good biocompatibility, and high manufacturing flexibility.…”

Section: Electrochemical Releasementioning

confidence: 99%

Section: Electrochemical Releasementioning

confidence: 99%

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Beyond Capture: Circulating Tumor Cell Release and Single‐Cell Analysis

Sharma

et al. 2019

Small Methods

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As the most promising “liquid biopsy”, analysis of circulating tumor cells (CTCs) provides noninvasive access to obtain biological information of solid tumors. It not only advances the understanding of the mechanisms underlying tumor metastasis, relapse, and chemoresistance, but also promotes the development of precision medicine. Over the past decade, the rapid advances in micro/nanomaterials and microfluidics have overcome the technical challenges of capturing CTCs, allowing efficient enrichment and sensitive detection. Beyond the capture of CTCs, major challenges of in‐depth CTC analysis are the release of CTCs from capture platforms and the inherent heterogeneity in CTCs. A variety of technologies have now emerged for release and single‐cell analysis of CTCs. This review focuses on recent progress along this direction. First, different release strategies are outlined and discussed, including their design principles and characteristics (merits and limitations). Then, existing methods for single CTC analysis at the genomic, transcriptomic, proteomic, and functional level are summarized. Finally, some perspectives are provided on current development trends, future research directions, and challenges of CTC studies.

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Hierarchical Spiky Microstraws‐Integrated Microfluidic Device for Efficient Capture and In Situ Manipulation of Cancer Cells

He¹,

Yang²,

Feng³

et al. 2019

Adv Funct Materials

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Techniques for capturing circulating tumor cells (CTCs) play an important role in cancer diagnosis. Recently, various 3D micro/nanostructures have been applied for effective CTC detection, yet in situ manipulation of the captured cancer cells on micro/nano‐structural substrates is rarely achieved. In this work, a hierarchical spiky microstraw array (HS‐MSA)‐integrated microfluidic device is demonstrated that possessed dual functions of cancer cell capture and in situ chemical manipulations of the captured cells. The 3D micro/nanostructure of HS‐MSA could capture cancer cells with high efficiency (≈84%) and strong specificity. Based on the HS‐MSA‐integrated microfluidic device, extracellular drug delivery to the captured cancer cells is achieved in situ with excellent spatial, dose, and temporal controls. In addition, a drug‐screening assay on the captured cancer cells is implemented to investigate the cell apoptosis behavior under the microstraw‐mediated delivery of staurosporine (STS). This microfluidic system not only presents tremendous potential for CTCs detection technology, but also opens up new opportunities for high‐throughput drug screening on cancer cells and understanding the cellular activity.

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Highly Efficient Capture and Electrochemical Release of Circulating Tumor Cells by Using Aptamers Modified Gold Nanowire Arrays

Cited by 70 publications

References 61 publications

Interfacing Neurons with Nanostructured Electrodes Modulates Synaptic Circuit Features

Interfacing Neurons with Nanostructured Electrodes Modulates Synaptic Circuit Features

Beyond Capture: Circulating Tumor Cell Release and Single‐Cell Analysis

Hierarchical Spiky Microstraws‐Integrated Microfluidic Device for Efficient Capture and In Situ Manipulation of Cancer Cells

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