Highly efficient detection of salbutamol in environmental water samples by an enzyme immunoassay

Liu, Zhenjiang; Zhang, Bo; Javed, Qaiser; Yi, Yinhui; Li, Ming; Du, Daolin; Zhu, Fang; Luan, Jiawen

doi:10.1016/j.scitotenv.2017.08.324

Cited by 22 publications

(14 citation statements)

References 22 publications

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“…In the overall development and application of immunoassays for OTA, immunochromatography has received extensive attention due to its advantages in simplicity, readability, and portability 19–22 . Shu et al .…”

Section: Introductionmentioning

confidence: 99%

“…18 In the overall development and application of immunoassays for OTA, immunochromatography has received extensive attention due to its advantages in simplicity, readability, and portability. [19][20][21][22] Shu et al used IC to measure OTA levels in food and animal feed with a sensitivity of 5 ng mL −1 and a detection time of only 10 min. 23 Li et al produced a multi-component chromatographic test strip for the simultaneous detection of AFB 1 , OTA, and ZEN, in which the LOD for OTA was 0.5 ng mL −1 .…”

Section: Introductionmentioning

confidence: 99%

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Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

et al. 2020

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BACKGROUND The toxicity and health risks of mycotoxins have encouraged increased awareness and strict monitoring of these contaminants in agricultural by‐products. In this paper, we developed and tested a sensitive, selective, and self‐assembling immunochromatographic (IC) strip for on‐site detection of ochratoxin A (OTA). We were able to demonstrate semi‐quantitative and quantitative detection of OTA in agricultural by‐product samples. RESULTS The optimized IC strip had a limit of detection (LOD) of 0.5 ng mL−1 OTA using the naked eye for semi‐quantitative detection. When a digitized strip reader was used to achieve quantitative results, the LOD for OTA was reduced to 0.1 ng mL−1 with a linear detection range of 0.1–10 ng mL−1. We also evaluated the specificity, stability, accuracy, and precision of the IC strip and demonstrated high performance in all of these areas. We then confirmed the ability of the IC strip to visually detect OTA contamination in authentic agricultural by‐product samples from the markets in China. Furthermore, quantitative detection of OTA using the IC strip showed that the concentration of OTA ranged from 1.7 to 101.3 ng g−1 in the positive agricultural by‐product samples, correlating well with the measurements obtained via liquid chromatography with tandem mass spectrometry. CONCLUSION The results indicated that this proposed IC strip was capable of sensitive, semi‐quantitative, quantitative, and on‐site detection of OTA contamination in agricultural by‐product samples. © 2020 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

et al. 2020

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“…The standard curve was established by fitting the inhibitory concentration value versus the concentration of aflatoxins using four parameter logistic regression. The LOD, sensitivity, and linear range were calculated according to previous research [30].…”

Section: Lod and Linear Rangementioning

confidence: 99%

A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

Zhang

Cai

et al. 2020

Toxins

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Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized Au@PtNPs and Au@PtNPs conjugated with a second antibody (Au@PtNPs-IgG). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. Au@PtNPs-IgG was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%–112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety.

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“…As a fundamental immunoassay, ELISA has been widely developed for detecting DON, given its simplicity, specificity, low cost, and ability to perform high-throughput screening [35,36,37]. Furthermore, rapid-detection technologies such as ELISA have received more attention; it is an alternative or complementary detection means that has been accepted by consumers and quarantine officers [38,39,40]. Due to the prevalence of mycotoxin contamination and the large number of samples that need to be analyzed, ELISA kits have been considered a suitable screening tool for the determination of mycotoxins, and their development and application has grown rapidly in recent years.…”

Section: Introductionmentioning

confidence: 99%

Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit

Sun

Xia

et al. 2018

Toxins

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A total of 328 agricultural product samples highly suspected to be contaminated, from flour companies, feed companies, and livestock farms throughout China, were surveyed for deoxynivalenol (DON) contamination using a self-assembly enzyme-linked immunosorbent assay (ELISA) kit. An ELISA kit for DON was developed with a 4.9 ng mL−1 limit of detection (LOD) in working buffer and a 200 ng g−1 LOD in authentic samples. The DON contamination detection rate was 88.7%, concentrations ranged from 200.9 to 6480.6 ng g−1, and the highest DON contamination was found in distillers’ dried grains with solubles with an average of 3204.5 ng g−1. Wheat bran and wheat were found to be the most commonly contaminated samples, and the corn meal samples had the lowest average DON level. This ELISA kit is a powerful alternative method for the rapid, sensitive, specific, accurate, and high-throughput determination of DON and can meet the maximum requirement levels. This survey suggests that DON contamination in the Chinese market is serious, and the contamination risk deserves attention. Essential preventive measures should be implemented to ensure food safety and human health.

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Highly efficient detection of salbutamol in environmental water samples by an enzyme immunoassay

Cited by 22 publications

References 22 publications

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

Semi‐quantitative and quantitative detection of ochratoxin A in agricultural by‐products using a self‐assembling immunochromatographic strip

A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

Survey of Deoxynivalenol Contamination in Agricultural Products in the Chinese Market Using An ELISA Kit

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