Highly efficient gene transfer with degradable poly(ester amine) based on poly(ethylene glycol) diacrylate and polyethylenimine in vitro and in vivo

Abstract

Our study demonstrates that poly(ester amine) may be applied as an useful gene carrier.

Nanomedicine in pulmonary delivery

“…100 To solve these problems, various modifications of PEI with liposomes/PEGs or conjugations of PEI with ligands such as transferrin have been investigated extensively. [101][102][103][104][105][106][107] Noninvasive pulmonary gene delivery using cationic polymers has been reviewed in detail elsewhere. 95 …”

Multi‐armed poly(L‐glutamic acid)‐graft‐oligoethylenimine copolymers as efficient nonviral gene delivery vectors

“…Although viral vectors can efficiently deliver therapeutic nucleotides into cells, the clinical application of these vectors is associated with considerable safety and immunogenicity risks 3, 4. More recently, cationic polymers have been increasingly investigated as safer alternatives for gene therapy 5–8 because of their potential advantages, such as the absence of a specific immune response, the large DNA loading capacity, and the flexibility to design a carrier with well‐defined structural and chemical properties on a large scale 9–12.…”

“…Usually, these PEI analogues are obtained by using a biodegradable crosslinker 6, 20, 23 or polymeric backbone 18, 21, 22. Although cationic polymer can be simply and quickly synthesized via a crosslinking reaction, it is difficult to control the synthetic process 23.…”

Medical Polymer-Based Gene Therapy

“…In addition, PAE-mediated gene expression in the lung and liver was higher than that of the conventional PEI carrier. Of particular interest, non-invasive aerosol delivery induced higher gene expression in all organs, compared with an intravenous method, in an in vivo mice study (Park, et al, 2008). The same group developed a new PAE based on hydrophobic polycaprolactone (PCL) and low molecular weight branched PEI following the Michael addition reaction .…”